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    NORMALIZATION OF NGS LIBRARY CONCENTRATION
    1.
    发明申请
    NORMALIZATION OF NGS LIBRARY CONCENTRATION 审中-公开
    NGS图书馆集中的规范化

    公开(公告)号：WO2018048957A2

    公开(公告)日：2018-03-15

    申请号：PCT/US2017/050354

    申请日：2017-09-06

    Applicant: SWIFT BIOSCIENCES, INC.

    Inventor： MAKAROV, Vladimir ,  CHUPRETA, Sergey

    IPC: C12Q1/68 ,  C07H21/04

    Abstract: A bottleneck in the Next Generation Sequencing (NGS) workflow is the quantification of libraries for accurate pooling and loading of the sequencing instrument flow cell or chip. Disclosed herein are methods that improve performance and reduce time compared to existing methods.

    Abstract translation: 新一代测序（NGS）工作流程中的一个瓶颈是对文库进行量化，以便精确合并和加载测序仪器流通池或芯片。 本文公开了与现有方法相比改进性能和减少时间的方法。

    IMPROVED METHODS FOR PROCESSING DNA SUBSTRATES
    2.
    发明申请
    IMPROVED METHODS FOR PROCESSING DNA SUBSTRATES 审中-公开
    改进DNA底物加工方法

    公开(公告)号：WO2015117040A1

    公开(公告)日：2015-08-06

    申请号：PCT/US2015/013994

    申请日：2015-01-30

    Applicant: SWIFT BIOSCIENCES, INC.

    Inventor： MAKAROV, Vladimir ,  LALIBERTE, Julie

    IPC: C12N15/11

    CPC classification number: C12Q1/686 ,  C12N15/66 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2521/501 ,  C12Q2521/525 ,  C12Q2521/531 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2535/122

    Abstract: The present disclosure describes a method of adapter ligation to the ends of fragmented double-stranded DNA molecules. This novel method overcomes the necessity to add a phosphate group to the 5' ends of DNA fragments. Instead, the 5' terminal bases that are damaged as a result of physical fragmentation of the DNA, are removed. By removal of the damaged base, a ligation compatible base with a 5' phosphate is exposed and adapter ligation efficiency is restored, leading to a significant increase in library yield and the ability to construct libraries from reduced input DNA quantities.

    Abstract translation: 本公开内容描述了将适配器连接到片段化双链DNA分子的末端的方法。 这种新方法克服了在DNA片段的5'末端添加磷酸基团的必要性。 相反，由于DNA的物理碎裂而损坏的5'末端碱基被去除。 通过去除损伤的碱，暴露具有5'磷酸盐的连接兼容碱基并恢复衔接子连接效率，导致文库产量显着增加，以及从减少的输入DNA量构建文库的能力。

    TEMPERATURE CONTROLLED DNA POLYMERASE INHIBITORS
    4.
    发明申请
    TEMPERATURE CONTROLLED DNA POLYMERASE INHIBITORS 审中-公开

    公开(公告)号：WO2020010124A3

    公开(公告)日：2020-01-09

    申请号：PCT/US2019/040367

    申请日：2019-07-02

    Applicant: SWIFT BIOSCIENCES, INC.

    Inventor： MAKAROV, Vladimir ,  ROSEFIGURA, Jordan ,  WOOD, Ashley, M.

    IPC: C12Q1/68 ,  C12P19/34 ,  G01N33/53

    Abstract: The present disclosure provides polynucleotide-based inhibitors for reversible activation of DNA polymerases. Use of lower Tm polynucleotide-based inhibitors allow PCR reaction assembly at room temperature while activating polymerase at higher PCR primer annealing temperatures, where the reversible nature of the inhibition additionally improves priming specificity during each PCR cycle. Additionally, temperature controlled inactivation of polymerase activity after PCR or other polymerase based enzymatic incubation eliminates a purification step when needed for compatibility with subsequent enzymatic incubations. For this application, the Tm of the polynucleotide-based inhibitor is higher than the desired reaction conditions of the subsequent enzymatic incubation.

    CLEAVABLE COMPETITOR POLYNUCLEOTIDES
    6.
    发明申请
    CLEAVABLE COMPETITOR POLYNUCLEOTIDES 审中-公开
    可清晰竞争的多边形竞争对手

    公开(公告)号：WO2015085183A3

    公开(公告)日：2015-09-24

    申请号：PCT/US2014068821

    申请日：2014-12-05

    Applicant: SWIFT BIOSCIENCES INC

    Inventor： MAKAROV VLADIMIR

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2525/137

    Abstract: The invention relates to polynucleotide combinations and their use in allele-specific enrichment, amplification, and detection. The disclosure also provides methods to multiplex various target DNA molecules in a single tube with high sensitivity and specificity. The disclosure provides a polynucleotide competitor that comprises a sequence that is fully complementary to a first target DNA polynucleotide region (T1 ) such that the competitor polynucleotide will hybridize to the first target DNA polynucleotide region under appropriate conditions. In another aspect, the polynucleotide competitor comprises a mismatch to a non-target DNA polynucleotide that is a sequence variant of the first target DNA polynucleotide region (T1*).

    Abstract translation: 本发明涉及多核苷酸组合及其在等位基因特异性富集，扩增和检测中的用途。 本公开还提供了以高灵敏度和特异性在单个管中复用多种靶DNA分子的方法。 本公开提供了多核苷酸竞争剂，其包含与第一靶DNA多核苷酸区域（T1）完全互补的序列，使得竞争性多核苷酸将在适当条件下与第一靶DNA多核苷酸区域杂交。 另一方面，多核苷酸竞争剂包含与作为第一靶DNA多核苷酸区域（T1 *）的序列变体的非靶DNA多核苷酸的错配。

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