公开(公告)号：WO0121760A9

公开(公告)日：2002-10-03

申请号：PCT/US0026122

申请日：2000-09-22

Applicant: CELL SCIENCE THERAPEUTICS INC ,  UPTON TODD M ,  FLICKINGER JOHN

Inventor： UPTON TODD M ,  FLICKINGER JOHN

IPC: C12M3/04 ,  C12M3/00

CPC classification number: C12M23/08 ,  C12M25/14 ,  C12M27/02

Abstract: The present invention relates to culturing devices that are compact, utilize small amounts of cell culture media to establish and maintain cell cultures, and produce a large number of cells in a short period of time when compared to other cell culturing devices and techniques. Such devices are useful in the culture of all cell types, but are particularly useful in the culture of cells that are known in the art to be difficult to culture, including cells that lose one or more of their particular attributes/characteristics (e.g., pluripotentiality), or cells that are difficult to establish cultures of (e.g. primary cells), during culturing in traditional cell culture devices.

Abstract translation: 本发明涉及紧凑的培养装置，利用少量的细胞培养基来建立和维持细胞培养物，并且与其它细胞培养装置和技术相比在短时间内产生大量的细胞。 这样的装置在所有细胞类型的培养中是有用的，但是在本领域已知的难以培养的细胞的培养中特别有用，包​​括丧失一个或多个其特定属性/特征的细胞（例如，多能性 ）或在传统细胞培养装置培养期间难以建立培养物（例如原代细胞）的细胞。

公开(公告)号：WO2012027687A8

公开(公告)日：2012-03-01

申请号：PCT/US2011/049379

申请日：2011-08-26

Applicant: CORNING INCORPORATED ,  DEROSA, Michael E ,  UPTON, Todd M ,  ZHANG, Ying

Inventor： DEROSA, Michael E ,  UPTON, Todd M ,  ZHANG, Ying

IPC: C08J7/02 ,  C12N5/00

Abstract: Thermoplastic polymeric sheets are rendered microporous and remain substantially flat by contacting the sheet with a first fluid composition that contains or more solvents for the polymeric sheet to render the sheet microporous and then contacting the microporous sheet with a second fluid composition that is substantially free of solvents for the polymer and that contains a non-solvent that is miscible with the one or more solvents of the first composition. Contacting the microporous sheet with the second fluid composition preferably occurs prior to substantial evaporation of the first fluid compositions, or solvents thereof, from the microporous sheet.

公开(公告)号：WO2008069902A3

公开(公告)日：2008-08-07

申请号：PCT/US2007023923

申请日：2007-11-14

Applicant: CORNING INC ,  MARTIN GREGORY R ,  TANNER ALLISON J ,  UPTON TODD M

Inventor： MARTIN GREGORY R ,  TANNER ALLISON J ,  UPTON TODD M

IPC: C12M1/00

CPC classification number: C12M23/34 ,  C12M23/14 ,  C12M23/24 ,  C12M29/04

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of flexible cell culture compartments in combination with a spacer material maintaining air space(s). The expandable compartments of the cell culture apparatus have imparted therein gas permeable membranes in combination with air spaces that will allow the free flow of gases between the cell culture compartments and the external environment. Furthermore an interconnecting passageway is provided between each compartment and between the cell culturing vessel and the external environment. The expandable vessel promotes the growth of large numbers of adherent or suspension cells by providing the volumes of nutrient medium and gaseous exchange.

Abstract translation: 公开了一种培养细胞的多层细胞培养装置。 细胞培养装置被定义为具有多个柔性细胞培养室与保持空气间隔的间隔物组合的整体结构。 细胞培养装置的可扩张隔室在其中赋予气体可渗透的膜与空气空间的组合，这将允许细胞培养室和外部环境之间的气体自由流动。 此外，在每个隔室之间和细胞培养容器与外部环境之间提供互连通道。 可膨胀的血管通过提供体积的营养培养基和气体交换来促进大量粘附或悬浮细胞的生长。

公开(公告)号：WO2003100010A3

公开(公告)日：2003-12-04

申请号：PCT/US2003/016419

申请日：2003-05-23

Applicant: CYTOMATRIX, LLC ,  PYKETT, Mark, J. ,  ROSENZWEIG, Michael ,  UPTON, Todd, M.

Inventor： PYKETT, Mark, J. ,  ROSENZWEIG, Michael ,  UPTON, Todd, M.

IPC: C12N5/00

Abstract: The invention pertains to methods and devices for the in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors. The hematopoietic progenitor cells are cultured in the absence of exogenously added hematopoietic growth factors without loss in cell progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency.

公开(公告)号：WO2012027687A1

公开(公告)日：2012-03-01

申请号：PCT/US2011049379

申请日：2011-08-26

Applicant: 4CORNING INC 1 ,  DEROSA MICHAEL E ,  UPTON TODD M ,  ZHANG YING

Inventor： DEROSA MICHAEL E ,  UPTON TODD M ,  ZHANG YING

IPC: C08J7/02 ,  C12N5/00

CPC classification number: C08J7/02 ,  C08J2325/06 ,  C08J2365/00

Abstract: Thermoplastic polymeric sheets are rendered microporous and remain substantially flat by contacting the sheet with a first fluid composition that contains or more solvents for the polymeric sheet to render the sheet microporous and then contacting the microporous sheet with a second fluid composition that is substantially free of solvents for the polymer and that contains a non-solvent that is miscible with the one or more solvents of the first composition. Contacting the microporous sheet with the second fluid composition preferably occurs prior to substantial evaporation of the first fluid compositions, or solvents thereof, from the microporous sheet.

Abstract translation: 将热塑性聚合物片材变成微孔并且通过使片材与含有或多于聚合物片材的溶剂的第一流体组合物接触而保持基本上平坦，以使片材微孔，然后使微孔片材与基本上不含溶剂的第二流体组合物接触 并且含有与第一组合物的一种或多种溶剂混溶的非溶剂。 在微孔片与第二流体组合物接触之前，优选在第一种流体组合物或其溶剂从微孔片大量蒸发之前进行。

公开(公告)号：WO2008069902A2

公开(公告)日：2008-06-12

申请号：PCT/US2007/023923

申请日：2007-11-14

Applicant: CORNING INCORPORATED ,  MARTIN, Gregory, R. ,  TANNER, Allison, J. ,  UPTON, Todd, M.

Inventor： MARTIN, Gregory, R. ,  TANNER, Allison, J. ,  UPTON, Todd, M.

IPC: C12M1/18

CPC classification number: C12M23/34 ,  C12M23/14 ,  C12M23/24 ,  C12M29/04

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of flexible cell culture compartments in combination with a spacer material maintaining air space(s). The expandable compartments of the cell culture apparatus have imparted therein gas permeable membranes in combination with air spaces that will allow the free flow of gases between the cell culture compartments and the external environment. Furthermore an interconnecting passageway is provided between each compartment and between the cell culturing vessel and the external environment. The expandable vessel promotes the growth of large numbers of adherent or suspension cells by providing the volumes of nutrient medium and gaseous exchange.

Abstract translation: 公开了用于培养细胞的多层细胞培养装置。 细胞培养设备被定义为具有多个柔性细胞培养隔室与保持空间的隔离材料相结合的整体结构。 细胞培养装置的可扩张隔室已经将气体渗透膜与允许气体在细胞培养隔室和外部环境之间自由流动的空间结合在一起。 此外，在每个隔室之间以及在细胞培养容器和外部环境之间提供互连通道。 可膨胀容器通过提供大量营养培养基和气体交换来促进大量粘附或悬浮细胞的生长。

公开(公告)号：WO2003100010A2

公开(公告)日：2003-12-04

申请号：PCT/US2003/016419

申请日：2003-05-23

Applicant: CYTOMATRIX, LLC ,  PYKETT, Mark, J. ,  ROSENZWEIG, Michael ,  UPTON, Todd, M.

Inventor： PYKETT, Mark, J. ,  ROSENZWEIG, Michael ,  UPTON, Todd, M.

IPC: C12N

CPC classification number: C12N5/0647

Abstract: The invention pertains to methods and devices for the in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors. The hematopoietic progenitor cells are cultured in the absence of exogenously added hematopoietic growth factors without loss in cell progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency.

Abstract translation: 本发明涉及在没有外源性添加的造血生长因子的情况下造血祖细胞的体外培养的方法和装置。 在没有外源性添加的造血生长因子的情况下培养造血祖细胞，而不损失细胞祖细胞数量和/或功能，同时保持祖细胞多能性。