公开(公告)号：WO2012039996A1

公开(公告)日：2012-03-29

申请号：PCT/US2011/051339

申请日：2011-09-13

Applicant: CORNING INCORPORATED ,  BENNETT, Scott Mathew ,  KENNEY, David A ,  MARTIN, Gregory R ,  SANTMAN, Jeffrey J. ,  TANNER, Allison J

Inventor： BENNETT, Scott Mathew ,  KENNEY, David A ,  MARTIN, Gregory R ,  SANTMAN, Jeffrey J. ,  TANNER, Allison J

IPC: C12M1/14

CPC classification number: C12M25/06 ,  C12M23/22 ,  C12M23/24 ,  C12M25/02 ,  C12M41/36

Abstract: The present invention relates generally to a multi-layer system for containing cells in culture, and a system for visualizing cells cultured in the multi-layered system. More specifically, the present invention relates to a multi-layer cell culture device having a specialized bottom plate and a microscope adaptor which can accommodate a microscope to allow microscopic visualization of cells cultured in the device.

Abstract translation: 本发明一般涉及用于在培养物中含有细胞的多层系统，以及用于在多层系统中培养的细胞的可视化系统。 更具体地说，本发明涉及一种具有专门的底板和显微镜适配器的多层细胞培养装置，其可以容纳显微镜以允许在装置中培养的细胞的显微镜观察。

公开(公告)号：WO2009128892A2

公开(公告)日：2009-10-22

申请号：PCT/US2009002302

申请日：2009-04-14

Applicant: CORNING INC ,  CATTADORIS HENRY J ,  MARTIN GREGORY R ,  TANNER ALLISON J

Inventor： CATTADORIS HENRY J ,  MARTIN GREGORY R ,  TANNER ALLISON J

IPC: C12M1/24

CPC classification number: C12M37/04 ,  C12M23/08 ,  C12M23/10 ,  C12M23/24 ,  C12M23/26 ,  C12M23/38 ,  C12M37/02

Abstract: An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir. The slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold. The filter is disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter.

Abstract translation: 用于培养细胞的组件包括限定用于容纳细胞培养基的储存器和与储存器流体连通的开口的壳体。 细胞培养组件还包括被配置为密封地接合开口的过滤阀组件。 过滤阀组件包括柔性膜，邻接侧壁，端壁和微生物过滤器。 端壁具有开口并且延伸穿过侧壁的一端以在侧壁和端壁内形成主腔。 柔性膜具有横跨膜的狭缝，并且在主空腔内是一次性的，使得膜将主腔密封地划分为（i）形成在端壁和膜之间的腔室和（ii）由膜形成的小腔 和侧壁的一部分。 小空腔与储存器流体连通。 狭缝被偏压在关闭位置，以防止流体从小空腔进入腔室，并被构造成打开，以允许气体在薄膜上的压差高于阈值时从小腔进入腔室。 过滤器设置在膜和开口之间，使得流过端壁中的开口进入室的空气通过过滤器。

公开(公告)号：WO2008106012A1

公开(公告)日：2008-09-04

申请号：PCT/US2008/001885

申请日：2008-02-13

Applicant: CORNING INCORPORATED ,  KENNEY, David, A. ,  MARTIN, Gregory, R. ,  TANNER, Allison, J. ,  WALL, Joseph, C.

Inventor： KENNEY, David, A. ,  MARTIN, Gregory, R. ,  TANNER, Allison, J. ,  WALL, Joseph, C.

IPC: C12M1/24 ,  C12M3/04 ,  B01L3/08

CPC classification number: B01L3/08 ,  C12M23/08 ,  C12M23/34 ,  C12M23/40 ,  C12M41/02

Abstract: A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.

Abstract translation: 公开了一种用于使细胞培养物中气泡或泡沫形成最小化的装置。 该装置具有将细胞和细胞培养基的流入引导到细胞培养容器中的歧管，以允许置换的空气或气体从细胞培养容器排出，而不与进入的细胞培养基混合，从而防止混合 空气和细胞培养基，并使细胞培养容器内泡沫或泡沫的形成最小化。

公开(公告)号：WO2008005244A2

公开(公告)日：2008-01-10

申请号：PCT/US2007/014852

申请日：2007-06-27

Applicant: CORNING INCORPORATED ,  MARTIN, Gregory R ,  TANNER, Allison J

Inventor： MARTIN, Gregory R ,  TANNER, Allison J

CPC classification number: C12M25/04 ,  B01L3/50255 ,  B01L3/50857 ,  B01L2300/0681 ,  B01L2300/0829 ,  C12M23/24 ,  C12M23/38

Abstract: A class of designs is provided for a permeable support where the design principle includes a permeable support layer, such as a membrane, bonded midway between two arrays of wells or multiwell plates, attached at their bottom portions, forming a double-sided multiwell plate. Thus, opposite facing wells on either side of the permeable support layer are accessible by inversion of the double-sided multiwell plate. Well fluid is held in place in the novel multiwell plate by capillary forces in the case of aligned upper and lower well arrays or by surface tension on patterned well regions on a permeable membrane layer. No additional components are necessary to form compartments for fluid retention. These new plate designs allow the surface area of the permeable support layer to be maximized, eliminate potential wicking and cross contamination issues that may arise from multiple component sidewalls, and take advantage of the small well diameters to retain fluid by utilizing the effects of surface tension or capillary forces.

Abstract translation: 提供了一类用于可渗透支撑的设计，其中设计原理包括可渗透的支撑层，例如膜，粘合在两个孔阵列或多孔板之间的中间，连接在其底部，形成双面多孔板。 因此，可渗透支撑层的任一侧上的相对面对的孔可通过双面多孔板的倒置进入。 在新颖的多孔板中，在对准的上下阵列的情况下通过毛细作用力将液体保持在适当的位置，或者通过可渗透膜层上的图案化阱区域上的表面张力来保持良好的流体。 不需要额外的部件来形成用于流体保持的隔室。 这些新的板设计允许可渗透支撑层的表面积最大化，消除可能由多个部件侧壁产生的潜在芯吸和交叉污染问题，并利用小井直径利用表面张力的作用来保持流体 或毛细管力。

公开(公告)号：WO2009094125A3

公开(公告)日：2010-08-26

申请号：PCT/US2009000303

申请日：2009-01-16

Applicant: CORNING INC ,  MARTIN GREGORY R ,  TANNER ALLISON J

Inventor： MARTIN GREGORY R ,  TANNER ALLISON J

IPC: C12M1/14

CPC classification number: C12M23/34 ,  C12M23/04 ,  C12M23/08 ,  C12M23/38 ,  C12M23/40

Abstract: The present invention provides a multi-layer cell culture device having a rectangular footprint and having multiple cell culture chambers separated by tracheal air spaces, each cell culture chamber having a port and a port cover or an external manifold structured and arranged to allow for transfer of fluid into and out of the cell culture device with reduced risk of contamination, and methods of using the device.

Abstract translation: 本发明提供了一种具有矩形足迹并具有由气管空气间隔开的多个细胞培养室的多层细胞培养装置，每个细胞培养室具有端口和端口盖或外部歧管，其构造和布置成允许 流入和流出细胞培养装置的污染风险降低，以及使用该装置的方法。

公开(公告)号：WO2010024887A1

公开(公告)日：2010-03-04

申请号：PCT/US2009/004843

申请日：2009-08-26

Applicant: CORNING INCORPORATED ,  MARTIN, Gregory, R ,  TANNER, Allison J

Inventor： MARTIN, Gregory, R ,  TANNER, Allison J

IPC: C12M1/00

CPC classification number: C12M23/24 ,  C12M23/14 ,  C12M23/34

Abstract: Cell culture apparatuses and methods for culturing cells include bags for culturing cells. The bags are folded and unfolded to control cell culture conditions. As the bags are folded, segmented sealed chambers for culturing cells are formed. As the bags are unfolded, the segments are unsealed allowing for exchange of fluid between the regions of the subchambers.

Abstract translation: 培养细胞的细胞培养装置和方法包括培养细胞的袋。 将袋折叠并展开以控制细胞培养条件。 当袋子被折叠时，形成用于培养细胞的分段密封室。 当袋子展开时，这些片段是开封的，允许在子房间的区域之间交换流体。

公开(公告)号：WO2009148504A1

公开(公告)日：2009-12-10

申请号：PCT/US2009/003108

申请日：2009-05-20

Applicant: CORNING INCORPORATED ,  CATTADORIS, Henry J. ,  POPOLOSKI, Martin J. ,  TANNER, Allison J.

Inventor： CATTADORIS, Henry J. ,  POPOLOSKI, Martin J. ,  TANNER, Allison J.

IPC: C12M1/00 ,  B01F11/00

CPC classification number: C12M23/14 ,  B01F11/0065 ,  C12M23/34 ,  C12M35/04

Abstract: A cell culture apparatus includes a housing(10), a bag(50a-e) and an inflatable bladder (40). The bag is disposed in the housing and has an interior surface defining a chamber for culturing cells. The bladder is disposed in the housing, external to the bag, and is sufficiently inflatable and expandable to exert pressure on the bag when the chamber is substantially free of fluid. The bladder may be inflated and expanded to reduce movement of the bag within the housing. Alternatively, or in addition, the bladder may be inflated and expanded to apply a mechanical stress on cells cultured within the chamber of the bag.

Abstract translation: 细胞培养装置包括壳体（10），袋（50a-e）和可膨胀囊（40）。 袋子设置在壳体中并具有限定用于培养细胞的室的内表面。 囊被布置在壳体的外部，并且当腔室基本上没有流体时，其充分膨胀和膨胀以对袋施加压力。 囊可以膨胀和膨胀以减少袋在壳体内的运动。 或者或另外，膀胱可以膨胀和膨胀，以将机械应力施加到在袋的腔室内培养的细胞上。

公开(公告)号：WO2009128892A3

公开(公告)日：2009-10-22

申请号：PCT/US2009/002302

申请日：2009-04-14

Applicant: CORNING INCORPORATED ,  CATTADORIS, Henry, J. ,  MARTIN, Gregory, R. ,  TANNER, Allison, J.

Inventor： CATTADORIS, Henry, J. ,  MARTIN, Gregory, R. ,  TANNER, Allison, J.

IPC: C12M1/12 ,  C12M1/22 ,  C12M1/24

Abstract: An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir. The slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold. The filter is disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter.

公开(公告)号：WO2009094125A2

公开(公告)日：2009-07-30

申请号：PCT/US2009/000303

申请日：2009-01-16

Applicant: CORNING INCORPORATED ,  MARTIN, Gregory, R. ,  TANNER, Allison, J.

Inventor： MARTIN, Gregory, R. ,  TANNER, Allison, J.

IPC: C12M1/18 ,  C12M3/04

CPC classification number: C12M23/34 ,  C12M23/04 ,  C12M23/08 ,  C12M23/38 ,  C12M23/40

Abstract: The present invention provides a multi-layer cell culture device having a rectangular footprint and having multiple cell culture chambers separated by tracheal air spaces, each cell culture chamber having a port and a port cover or an external manifold structured and arranged to allow for transfer of fluid into and out of the cell culture device with reduced risk of contamination, and methods of using the device.

Abstract translation: 本发明提供了一种具有矩形足迹并具有由气管空气间隔开的多个细胞培养室的多层细胞培养装置，每个细胞培养室具有端口和端口盖或外部歧管，其构造和布置成允许 流入和流出细胞培养装置的污染风险降低，以及使用该装置的方法。

公开(公告)号：WO2008153783A1

公开(公告)日：2008-12-18

申请号：PCT/US2008/006693

申请日：2008-05-27

Applicant: CORNING INCORPORATED ,  MARTIN, Gregory, R. ,  TANNER, Allison, J.

Inventor： MARTIN, Gregory, R. ,  TANNER, Allison, J.

IPC: C12M3/06

CPC classification number: C12M23/24 ,  C12M23/34 ,  C12M23/38 ,  C12M23/40 ,  C12M29/04 ,  C12M35/08

Abstract: A multilayered cell culture apparatus for the co-culturing of at least two cell types is disclosed. The cell co-culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between some cell culture chambers and the external environment. In addition, some cell culture chambers may have limited access to air creating relatively lower oxygen content cell culture chambers. The size of the apparatus, and location of an optional neck section, allows for its manipulation by standard automated assay equipment, further making the apparatus amenable to high throughput applications.

Abstract translation: 公开了用于共培养至少两种细胞类型的多层细胞培养装置。 细胞共培养装置被定义为具有与气管空间组合的多个细胞培养室的整体结构。 该装置的主体在其中赋予气体可渗透的膜与气管空间的组合，这将允许气体在一些细胞培养室和外部环境之间自由流动。 此外，一些细胞培养室可能有限制地进入空气，产生相对较低的含氧细胞培养室。 设备的尺寸和可选的颈部的位置允许其通过标准自动化测定设备的操作，进一步使得该设备适合于高通量应用。