公开(公告)号：WO2012122017A3

公开(公告)日：2012-11-01

申请号：PCT/US2012027477

申请日：2012-03-02

Applicant: UNIV CORNELL ,  UNIV ROCKEFELLER ,  ELEMENTO OLIVIER ,  KAPOOR TARUN M ,  WACKER SARAH A

Inventor： ELEMENTO OLIVIER ,  WACKER SARAH A

IPC: C12Q1/68

CPC classification number: G06F19/16 ,  C12Q1/6874 ,  G01N33/5008 ,  G01N2800/44

Abstract: A method of identification of drug targets and drug resistance mechanisms in human cells of a drug comprising the steps of: generating at least one drug-resistant sample and at least one drug-sensitive sample; analyzing substantial portions of the genome and/or transcriptome of the least one drug-resistant sample and drug-sensitive sample to obtain sequencing data; detecting substantially all alterations in the at least drug-resistant sample; deriving a resistance signature; and performing analysis of the drug resistance signature of at least one recurrently altered gene using bioinformatic tools and cellular biology methods to determine if alteration of the at least one gene of the drug resistance signature is sufficient to confer at least partial resistance to cells or tissues against the drug.

Abstract translation: 一种在药物人体细胞中鉴定药物靶标和耐药机制的方法，包括以下步骤：产生至少一种耐药样品和至少一种药物敏感样品; 分析至少一种药物抗性样品和药物敏感样品的基因组和/或转录组的大部分，以获得测序数据; 检测至少耐药性样品中的所有改变; 导出电阻签名; 以及使用生物信息学工具和细胞生物学方法进行至少一个循环改变的基因的耐药性特征的分析，以确定抗药性特征的至少一个基因的改变是否足以赋予对细胞或组织的至少部分抗性以抵抗 毒品。

公开(公告)号：WO2012122017A2

公开(公告)日：2012-09-13

申请号：PCT/US2012/027477

申请日：2012-03-02

Applicant: CORNELL UNIVERSITY ,  THE ROCKEFELLER UNIVERSITY ,  ELEMENTO, Olivier ,  KAPOOR, Tarun M. ,  WACKER, Sarah A.

Inventor： ELEMENTO, Olivier ,  WACKER, Sarah A.

IPC: C12Q1/68

CPC classification number: G06F19/16 ,  C12Q1/6874 ,  G01N33/5008 ,  G01N2800/44

Abstract: A method of identification of drug targets and drug resistance mechanisms in human cells of a drug comprising the steps of: generating at least one drug-resistant sample and at least one drug-sensitive sample; analyzing substantial portions of the genome and/or transcriptome of the least one drug-resistant sample and drug-sensitive sample to obtain sequencing data; detecting substantially all alterations in the at least drug-resistant sample; deriving a resistance signature; and performing analysis of the drug resistance signature of at least one recurrently altered gene using bioinformatic tools and cellular biology methods to determine if alteration of the at least one gene of the drug resistance signature is sufficient to confer at least partial resistance to cells or tissues against the drug.

Abstract translation: 一种鉴定药物人类细胞中的药物靶标和药物抗性机制的方法，包括以下步骤：产生至少一种耐药样品和至少一种药物敏感样品; 分析至少一种耐药样品和药物敏感样品的基因组和/或转录组的大部分，以获得测序数据; 检测至少耐药样品中的基本上全部改变; 导出抵制签名; 以及使用生物信息学工具和细胞生物学方法对至少一种经常改变的基因的药物抗性标记进行分析，以确定所述药物抗性标记的至少一个基因的改变是否足以赋予针对细胞或组织的至少部分抗性 该药。