公开(公告)号：WO2008092081A3

公开(公告)日：2009-02-12

申请号：PCT/US2008052054

申请日：2008-01-25

Applicant: IMMUNE DISEASE INST INC ,  SHANKAR PREMLATA ,  LEE SANG-KYUNG ,  SWAMY MANJU ,  KUMAR PRITI ,  WU HAOQUAN ,  BAN HONG-SEOK

Inventor： SHANKAR PREMLATA ,  LEE SANG-KYUNG ,  SWAMY MANJU ,  KUMAR PRITI ,  WU HAOQUAN ,  BAN HONG-SEOK

IPC: C12N15/11 ,  C12N15/113 ,  C12N15/115

CPC classification number: C12N15/111 ,  A61K48/00 ,  C12N15/1135 ,  C12N15/1136 ,  C12N15/1138 ,  C12N15/115 ,  C12N2310/14 ,  C12N2310/3513 ,  C12N2320/32

Abstract: The present invention provides a method of delivering RNA interference molecules to a cell or a cell in a subject, which comprises contacting the cell with a protein-double stranded RNA complex, the complex comprising the double stranded RNA segment containing a double stranded RNA of interest and a protein, the protein comprising (1) a targeting moiety, which will specifically bind to a site on a target cell, and (2) a binding moiety linked thereto, which will bind to the double stranded RNA, wherein the double stranded RNA segment is delivered to a cell and effects RNA interference of the target RNA in the cell.

Abstract translation: 本发明提供了将RNA干扰分子递送至受试者中的细胞或细胞的方法，其包括使细胞与蛋白质 - 双链RNA复合物接触，所述复合物包含含有感兴趣的双链RNA的双链RNA节段 和蛋白质，所述蛋白质包含（1）将与靶细胞上的位点特异性结合的靶向部分，和（2）与其连接的结合部分，其将结合双链RNA，其中双链RNA 片段被递送至细胞并影响细胞中靶RNA的RNA干扰。

公开(公告)号：WO2008092081A2

公开(公告)日：2008-07-31

申请号：PCT/US2008/052054

申请日：2008-01-25

Applicant: IMMUNE DISEASE INSTITUTE, INC. ,  SHANKAR, Premlata ,  LEE, Sang-kyung ,  SWAMY, Manju ,  KUMAR, Priti ,  WU, Haoquan ,  BAN, Hong-Seok

Inventor： SHANKAR, Premlata ,  LEE, Sang-kyung ,  SWAMY, Manju ,  KUMAR, Priti ,  WU, Haoquan ,  BAN, Hong-Seok

IPC: A61K48/00

CPC classification number: C12N15/111 ,  A61K48/00 ,  C12N15/1135 ,  C12N15/1136 ,  C12N15/1138 ,  C12N15/115 ,  C12N2310/14 ,  C12N2310/3513 ,  C12N2320/32

Abstract: The present invention provides a method of delivering RNA interference molecules to a cell or a cell in a subject, which comprises contacting the cell with a protein-double stranded RNA complex, the complex comprising the double stranded RNA segment containing a double stranded RNA of interest and a protein, the protein comprising (1) a targeting moiety, which will specifically bind to a site on a target cell, and (2) a binding moiety linked thereto, which will bind to the double stranded RNA, wherein the double stranded RNA segment is delivered to a cell and effects RNA interference of the target RNA in the cell.

Abstract translation: 本发明提供了将RNA干扰分子递送至受试者的细胞或细胞的方法，该方法包括使细胞与蛋白质双链RNA复合物接触，所述复合物包含含有目的双链RNA的双链RNA片段 和蛋白质，所述蛋白质包含（1）将特异性结合靶细胞上的位点的靶向部分，和（2）与所述双链RNA结合的与其连接的结合部分，其中所述双链RNA 片段被递送到细胞并且影响目标RNA在细胞中的RNA干扰。

公开(公告)号：WO2008092081A8

公开(公告)日：2009-07-16

申请号：PCT/US2008052054

申请日：2008-01-25

Applicant: IMMUNE DISEASE INST INC ,  UNIV SOGANG IND UNIV COOP FOUN ,  SHANKAR PREMLATA ,  LEE SANG-KYUNG ,  SWAMY MANJU ,  KUMAR PRITI ,  WU HAOQUAN ,  BAN HONG-SEOK

Inventor： SHANKAR PREMLATA ,  LEE SANG-KYUNG ,  SWAMY MANJU ,  KUMAR PRITI ,  WU HAOQUAN ,  BAN HONG-SEOK

IPC: C12N15/11 ,  C12N15/113 ,  C12N15/115

CPC classification number: C12N15/111 ,  A61K48/00 ,  C12N15/1135 ,  C12N15/1136 ,  C12N15/1138 ,  C12N15/115 ,  C12N2310/14 ,  C12N2310/3513 ,  C12N2320/32

Abstract: The present invention provides a method of delivering RNA interference molecules to a cell or a cell in a subject, which comprises contacting the cell with a protein-double stranded RNA complex, the complex comprising the double stranded RNA segment containing a double stranded RNA of interest and a protein, the protein comprising (1) a targeting moiety, which will specifically bind to a site on a target cell, and (2) a binding moiety linked thereto, which will bind to the double stranded RNA, wherein the double stranded RNA segment is delivered to a cell and effects RNA interference of the target RNA in the cell.

Abstract translation: 本发明提供了将RNA干扰分子递送至受试者的细胞或细胞的方法，该方法包括使细胞与蛋白质双链RNA复合物接触，所述复合物包含含有目的双链RNA的双链RNA片段 和蛋白质，所述蛋白质包含（1）将特异性结合靶细胞上的位点的靶向部分，和（2）与所述双链RNA结合的与其连接的结合部分，其中所述双链RNA 片段被递送到细胞并且影响目标RNA在细胞中的RNA干扰。

公开(公告)号：WO2016061232A2

公开(公告)日：2016-04-21

申请号：PCT/US2015/055537

申请日：2015-10-14

Applicant: TEXAS TECH UNIVERSITY SYSTEM ,  WU, Haoquan ,  CHOI, Jang-gi

Inventor： WU, Haoquan ,  CHOI, Jang-gi

IPC: A61K48/00 ,  C07H21/02

CPC classification number: C12N15/1132 ,  A61K48/005 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/31 ,  C12N2310/3519 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/51 ,  C12N2740/15042 ,  C12N2750/14142

Abstract: Nucleic acid molecules such as shRNA clusters and artificial miRNA dusters are disclosed. Also disclosed are methods of use, compositions, cells, viral particles, and kits relating to the nucleic acid molecules disclosed herein.

Abstract translation: 公开了核酸分子如shRNA簇和人造miRNA除尘器。 还公开了与本文公开的核酸分子有关的使用方法，组合物，细胞，病毒颗粒和试剂盒。

公开(公告)号：WO2016061232A3

公开(公告)日：2016-08-11

申请号：PCT/US2015055537

申请日：2015-10-14

Applicant: UNIV TEXAS TECH SYSTEM ,  WU HAOQUAN ,  CHOI JANG-GI

Inventor： WU HAOQUAN ,  CHOI JANG-GI

IPC: A61K31/7105 ,  C12N15/11 ,  C12N15/113

CPC classification number: C12N15/1132 ,  A61K48/00 ,  A61K48/005 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/31 ,  C12N2310/3519 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/51

Abstract: Nucleic acid molecules such as shRNA clusters and artificial miRNA clusters are disclosed, Also disclosed are methods of use, compositions, cells, viral particles, and kits relating to the nucleic acid molecules disclosed herein. The disclosure provides, at least in part nucleic acid molecules such as shRNA clusters encoding shRNA-like molecules and artificial miRNA clusters encoding modified pri-miRNA-like molecules. The shRNA clusters and artificial miRNA clusters disclosed herein can be used, for example, to produce artificial RNA molecules, e.g., RNAi molecules. Cells, viral particles, compositions (e.g., pharmaceutical compositions), kits, and methods relating to the nucleic acid molecules, e.g., shRNA clusters and artificial miRNA clusters, are also disclosed. The nucleic acid molecules (e.g., shRNA clusters and artificial miRNA clusters), artificial RNA molecules (e.g., RNAi molecules), cells, viral particles, compositions (e.g., pharmaceutical compositions), and kits and methods disclosed herein can be used to treat or prevent a disease, e.g., HIV infection and/or AIDS.

Abstract translation: 公开了核酸分子，例如shRNA簇和人工miRNA簇。还公开了与本文公开的核酸分子有关的使用方法，组合物，细胞，病毒颗粒和试剂盒。 本公开提供至少部分核酸分子，例如编码shRNA样分子的shRNA簇和编码修饰的pri-miRNA样分子的人工miRNA簇。 本文公开的shRNA簇和人工miRNA簇可用于例如人造RNA分子，例如RNAi分子。 还公开了细胞，病毒颗粒，组合物（例如药物组合物），试剂盒和与核酸分子有关的方法，例如shRNA簇和人工miRNA簇。 本文公开的核酸分子（例如，shRNA簇和人工miRNA簇），人造RNA分子（例如RNAi分子），细胞，病毒颗粒，组合物（例如药物组合物）和试剂盒和方法可用于治疗或 预防艾滋病毒/艾滋病等疾病。