公开(公告)号：WO2008143956A1

公开(公告)日：2008-11-27

申请号：PCT/US2008/006263

申请日：2008-05-15

Applicant: CODEXIS, INC. ,  GLIEDER, Anton ,  SCHITTMAYER, Matthias ,  KAMBOURAKIS, Spiros ,  ZACH, Simone

Inventor： GLIEDER, Anton ,  SCHITTMAYER, Matthias ,  KAMBOURAKIS, Spiros ,  ZACH, Simone

IPC: C12P1/00

CPC classification number: C12P33/06 ,  C12P7/26

Abstract: A method of selective biooxidation to non activated carbon-hydrogen bonds of substances using a Geobacillus kaustophilus 'Old Yellow Enzyme1 is provided". It is shown that OYEs can be used to facilitate the biooxydation of substances, such as testosterone. It is also shown that OYE can introduce double bonds to form alpha, betaalpha, beta desaturated ketones. Furthermore, it is also shown that the use of OYEs allows for the production of oxidized substances in one step reactions, which are otherwise not accessible or only accessible after complex and inefficient multi-step reactions. In addition, the OYE used shows high stability (e.g. at high temperature, or in long lasting bioconversions). An exemplary embodiment is provided showing the use of an OYE to convert testosterone to 6α- hydroxytestosterone.

Abstract translation: 提供了一种利用土壤杆菌“老黄酶1”对物质的非活性碳氢键进行选择性生物氧化的方法“，表明OYE可用于促进物质如睾酮的生物氧化，还显示 OYE可以引入双键形成α，βalpha，β去饱和酮，此外，还表明，使用OYE允许在一步反应中生产氧化物质，否则它们不可接近或仅在复杂和低效后才能进入 此外，所用的OYE显示出高稳定性（例如在高温或长期生物转化中）。提供了示例性实施方案，其显示使用OYE将睾酮转化为6a-羟基睾酮。