公开(公告)号：WO2008137789A1

公开(公告)日：2008-11-13

申请号：PCT/US2008/062563

申请日：2008-05-02

Applicant: CODEXIS, INC. ,  GLIEDER, Anton ,  SCHITTMAYER, Matthias

Inventor： GLIEDER, Anton ,  SCHITTMAYER, Matthias

IPC: C12P7/06 ,  C12C11/00

CPC classification number: C12P33/00 ,  Y02E50/17

Abstract: A method for a chemoselective and regioselective enzyme mediated oxidation of carbon-hydrogen bonds of substrates using a Geobacillus kaustophilus 'Old Yellow Enzyme' is provided. It is shown that OYEs can be used to facilitate the bioxidation of substrates, such as testosterone. It is also shown that the use of OYEs allows for the production of oxidized substrates in one step reactions, which are otherwise not accessible or only accessible after complex and inefficient multi-step reactions. In addition, the OYE used shows high stability at high temperature. An exemplary embodiment is provided showing the use of an OYE to convert testosterone to 6α- hydroxytestosterone.

Abstract translation: 提供了一种化学选择性和区域选择性酶介导的氧化碳底物的碳氢键的方法，该方法是使用耐火土芽孢杆菌“老黄酶”。 显示OYE可用于促进底物的生物氧化，例如睾酮。 还显示，使用OYE允许在一步反应中生产氧化的底物，否则其不可接近或仅在复杂和无效的多步反应之后才能使用。 此外，所使用的OYE在高温下显示高稳定性。 提供了示例性实施方案，其显示使用OYE将睾酮转化为6a-羟基睾酮。

公开(公告)号：WO2008143956A1

公开(公告)日：2008-11-27

申请号：PCT/US2008/006263

申请日：2008-05-15

Applicant: CODEXIS, INC. ,  GLIEDER, Anton ,  SCHITTMAYER, Matthias ,  KAMBOURAKIS, Spiros ,  ZACH, Simone

Inventor： GLIEDER, Anton ,  SCHITTMAYER, Matthias ,  KAMBOURAKIS, Spiros ,  ZACH, Simone

IPC: C12P1/00

CPC classification number: C12P33/06 ,  C12P7/26

Abstract: A method of selective biooxidation to non activated carbon-hydrogen bonds of substances using a Geobacillus kaustophilus 'Old Yellow Enzyme1 is provided". It is shown that OYEs can be used to facilitate the biooxydation of substances, such as testosterone. It is also shown that OYE can introduce double bonds to form alpha, betaalpha, beta desaturated ketones. Furthermore, it is also shown that the use of OYEs allows for the production of oxidized substances in one step reactions, which are otherwise not accessible or only accessible after complex and inefficient multi-step reactions. In addition, the OYE used shows high stability (e.g. at high temperature, or in long lasting bioconversions). An exemplary embodiment is provided showing the use of an OYE to convert testosterone to 6α- hydroxytestosterone.

Abstract translation: 提供了一种利用土壤杆菌“老黄酶1”对物质的非活性碳氢键进行选择性生物氧化的方法“，表明OYE可用于促进物质如睾酮的生物氧化，还显示 OYE可以引入双键形成α，βalpha，β去饱和酮，此外，还表明，使用OYE允许在一步反应中生产氧化物质，否则它们不可接近或仅在复杂和低效后才能进入 此外，所用的OYE显示出高稳定性（例如在高温或长期生物转化中）。提供了示例性实施方案，其显示使用OYE将睾酮转化为6a-羟基睾酮。