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    THERMOTOGA NEAPOLITANA DSM 5068 ALKALINE PHOSPHATASE (PHOA) GENE AND RECOMBINANT ENZYME PRODUCTION
    1.
    发明申请
    THERMOTOGA NEAPOLITANA DSM 5068 ALKALINE PHOSPHATASE (PHOA) GENE AND RECOMBINANT ENZYME PRODUCTION 审中-公开
    THERMOTOGA NEAPOLITANA DSM 5068碱性磷酸酶（PHOA）基因和重组酶生产

    公开(公告)号：WO2008042235A2

    公开(公告)日：2008-04-10

    申请号：PCT/US2007020888

    申请日：2007-09-28

    Applicant: UNIV MICHIGAN STATE ,  VIEILLE CLAIRE ,  ZEIKUS J GREGORY ,  LAIVENIEKS MARIS

    Inventor： VIEILLE CLAIRE ,  ZEIKUS J GREGORY ,  LAIVENIEKS MARIS

    IPC: C12N9/16

    CPC classification number: C12N9/16

    Abstract: The present invention relates to compositions and methods for providing a recombinant thermostable Thermotoga neapolitana alkaline phosphatase enzyme. More particularly, the invention relates to engineering Escherichia coli with T. neapolitana alkaline phosphatase gene expression vectors for providing an inducible system for thermostable enzyme production, wherein the expressed enzyme is readily soluble with a high degree of activity. These methods provide for commercial quantities of a thermostable alkaline phosphatase enzyme.

    Abstract translation: 本发明涉及用于提供重组热稳定性Thermotoga neapolitana碱性磷酸酶的组合物和方法。 更具体地，本发明涉及用于提供用于热稳定酶生产的诱导型系统的具有T. neapolitana碱性磷酸酶基因表达载体的大肠杆菌的工程，其中所表达的酶易于以高度的活性溶解。 这些方法提供商业量的耐热碱性磷酸酶。

    MOLECULAR DESIGN OF THERMOSTABLE ALCOHOL DEHYDROGENASE FOR SYNTHESIS FOR CHIRAL AROMATIC ALCOHOLS
    2.
    发明申请
    MOLECULAR DESIGN OF THERMOSTABLE ALCOHOL DEHYDROGENASE FOR SYNTHESIS FOR CHIRAL AROMATIC ALCOHOLS 审中-公开
    用于合成芳香醇的可热解酒精脱水酶的分子设计

    公开(公告)号：WO2008013949A2

    公开(公告)日：2008-01-31

    申请号：PCT/US2007016929

    申请日：2007-07-27

    Applicant: UNIV MICHIGAN STATE ,  VIEILLE CLAIRE ,  ZEIKUS J GREGORY ,  ZIEGELMANN-FJELD KARLA I

    Inventor： VIEILLE CLAIRE ,  ZEIKUS J GREGORY ,  ZIEGELMANN-FJELD KARLA I

    IPC: C12P7/22

    CPC classification number: C12P7/02 ,  C12N9/0006 ,  C12P7/16 ,  C12P7/22 ,  C12P41/002 ,  Y02E50/10

    Abstract: The present invention relates to compositions and methods utilizing thermostable and novel alcohol dehydrogenase enzymes for biosynthesizing chiral specific molecules for use as precursor molecules in synthesizing pharmaceutical compounds. Particularly, in preferred embodiments, the invention relates to directed engineering of an enzymatic catalytic site of an alcohol dehydrogenase enzyme gene for enhancing enantioselectivity for (S)-enantiomer substrate catalytic activity for providing aryl (S)-enantiomer products in stereomeric excess.

    Abstract translation: 本发明涉及利用热稳定和新型醇脱氢酶用于生物合成用于合成药物化合物的前体分子的手性特异性分子的组合物和方法。 特别地，在优选实施方案中，本发明涉及用于提高（S） - 对映异构体底物催化活性以提供立体异构过量的芳基（S） - 对映体产物的对映体选择性的醇脱氢酶基因的酶催化位点的定向工程。

    MINIMAL GROWTH MEDIUM FOR ACTINOBACILLUS SUCCINOGENES
    4.
    发明申请
    MINIMAL GROWTH MEDIUM FOR ACTINOBACILLUS SUCCINOGENES 审中-公开
    ACTINOBACILLUS SUCCINEMENES的最小生长培养基

    公开(公告)号：WO2007035589A1

    公开(公告)日：2007-03-29

    申请号：PCT/US2006/036206

    申请日：2006-09-15

    Applicant: MICHIGAN STATE UNIVERSITY ,  ZEIKUS, J., Gregory ,  VIEILLE, Claire ,  MCKINLAY, James, B.

    Inventor： ZEIKUS, J., Gregory ,  VIEILLE, Claire ,  MCKINLAY, James, B.

    IPC: A01N63/00 ,  C12N1/00 ,  C12N1/20

    CPC classification number: C12N1/20

    Abstract: A minimal defined medium for growth of A. succinogenes in an anaerobic environment is described. The medium expands the experimental opportunities for studying the metabolism of A. succinogenes, including 13 C-labeling experiments . The medium comprises essential amino acids . Other ingredients are also essential such as glucose or another carbohydrate as the primary carbon source, minerals, and vitamins . The medium allows for 13 C-labelling experiments that can reveal the metabolic pathways used by A. succinogenes and the extent to which they are used, including those pathways involved in succinate production

    Abstract translation: 描述了在厌氧环境中用于生产琥珀酸原子的最小限定的培养基。 该培养基扩大了研究A.succinogenes代谢的实验机会，其中包括13 C-标记实验。 培养基包含必需氨基酸。 其他成分也是必需的，如葡萄糖或其他碳水化合物作为主要碳源，矿物质和维生素。 该培养基允许可以揭示由A.succinogenes使用的代谢途径及其使用程度的包括琥珀酸生产中涉及的那些途径的<13 C>标签实验

    PNEUMATIC BIOREACTOR
    5.
    发明申请
    PNEUMATIC BIOREACTOR 审中-公开
    气动生物反应器

    公开(公告)号：WO2007111677A3

    公开(公告)日：2008-10-23

    申请号：PCT/US2006060183

    申请日：2006-10-24

    Applicant: PBS BIOTECH INC ,  ZEIKUS J GREGORY

    Inventor： ZEIKUS J GREGORY

    IPC: B01F13/02

    CPC classification number: B01F3/04113 ,  B01F3/04602 ,  B01F7/00483 ,  B01F11/0082 ,  B01F15/005 ,  B01F15/00525 ,  C12M23/56 ,  C12M27/02 ,  C12M27/06

    Abstract: A pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls In the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides. In another embodiment, floating pistons mix the fluid, pushing it through a mixing plate with one or more apertures. In a third embodiment, the mixing device is a rotating drum vvith bubble-catching blades and rotating mixing plates with apertures. The top of the vessel for these mixers may include a closed top and sterile filters.

    Abstract translation: 气动生物反应器包括容纳待混合的流体的容器和由气体压力驱动的至少一个混合装置。 第一实施例包括浮动叶轮，其随着气泡将其向上运送到气体然后被排出的表面而在流体中，允许叶轮沉入流体中。 浮动叶轮可以用柔性构件和滑轮系在第二叶轮上。 混合速度由容器中的电磁体控制，作用在叶轮或其引导件中的磁性材料上。 在另一个实施例中，浮动活塞将流体混合，将其推入具有一个或多个孔的混合板。 在第三实施例中，混合装置是带有吸气片的旋转鼓和具有孔的旋转混合板。 用于这些混合器的容器的顶部可以包括封闭的顶部和无菌过滤器。

    PNEUMATIC BIOREACTOR
    6.
    发明申请
    PNEUMATIC BIOREACTOR 审中-公开
    气动生物反应器

    公开(公告)号：WO2008133845A3

    公开(公告)日：2009-07-02

    申请号：PCT/US2008005010

    申请日：2008-04-18

    Applicant: PBS BIOTECH INC ,  ZEIKUS J GREGORY ,  KIM KYUNGNAM ,  DICKEY DAVID S

    Inventor： ZEIKUS J GREGORY ,  KIM KYUNGNAM ,  DICKEY DAVID S

    IPC: C12M1/02 ,  C12M1/06

    CPC classification number: C12M27/06

    Abstract: A pneumatic bioreactor having a containment vessel which includes a semi-cylindrical concavity defined by the vessel bottom. A mixing apparatus includes a rotational mixer rotatably mounted within the containment vessel about a horizontal axis. The rotational mixer has buoyancy-driven mixing cavities which are fed by a gas supply beneath the rotational mixer. The mixing apparatus extends into the semi- cylindrical concavity to substantially fill that concavity. The rotational mixer is divided into two wheels with outer paddles extending axially outwardly and inner paddles extending axially inwardly on either side of each ring. Blades between the outer and inner paddles form impellers in the wheels to induce axial flow through the rings in opposite directions. The containment vessel may be of film and supported by a structural housing also having a semi-cylindrical concavity defined by the housing bottom.

    Abstract translation: 一种具有容纳容器的气动生物反应器，其包括由容器底部限定的半圆柱形凹面。 混合装置包括旋转混合器，其围绕水平轴可旋转地安装在容纳容器内。 旋转混合器具有浮力驱动的混合腔，其通过旋转混合器下面的气体供给进料。 混合装置延伸到半圆柱形凹部中以基本上填充该凹面。 旋转混合器被分成两个轮，其外侧叶片沿轴向向外延伸，内侧桨叶在每个环的任一侧上轴向向内延伸。 外桨叶和内桨叶之间的叶片在轮中形成叶轮以引起轴向流动通过环的相反方向。 安全壳可以是薄膜，并由具有由壳体底部限定的半圆柱形凹面的结构外壳支撑。

    PNEUMATIC BIOREACTOR
    8.
    发明申请
    PNEUMATIC BIOREACTOR 审中-公开
    气动生物反应器

    公开(公告)号：WO2007111677A2

    公开(公告)日：2007-10-04

    申请号：PCT/US2006/060183

    申请日：2006-10-24

    Applicant: PBS BIOTECH, INC ,  ZEIKUS, J., Gregory

    Inventor： ZEIKUS, J., Gregory

    IPC: B01F13/00

    CPC classification number: B01F3/04113 ,  B01F3/04602 ,  B01F7/00483 ,  B01F11/0082 ,  B01F15/005 ,  B01F15/00525 ,  C12M23/56 ,  C12M27/02 ,  C12M27/06

    Abstract: A pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls In the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides. In another embodiment, floating pistons mix the fluid, pushing it through a mixing plate with one or more apertures. In a third embodiment, the mixing device is a rotating drum vvith bubble-catching blades and rotating mixing plates with apertures. The top of the vessel for these mixers may include a closed top and sterile filters.

    Abstract translation: 气动生物反应器包括容纳待混合的流体的容器和由气体压力驱动的至少一个混合装置。 第一实施例包括浮动叶轮，其在气泡将其向上运送到气体然后被排出的表面时在流体中，允许叶轮沉入流体中。 浮动叶轮可以用柔性构件和滑轮系在第二叶轮上。 混合速度由容器中的电磁体控制，作用在叶轮或其引导件中的磁性材料上。 在另一个实施例中，浮动活塞混合流体，将其推入具有一个或多个孔的混合板。 在第三实施例中，混合装置是带有吸气片的旋转鼓和具有孔的旋转混合板。 用于这些混合器的容器的顶部可以包括封闭的顶部和无菌过滤器。

    GENES FROM ACTINOBACILLUS SUCCINOGENES 13OZ (ATCC 55618) FOR PRODUCTION OF CHEMICALS FROM THE A. SUCCINOGENES C4-PATHWAY
    9.
    发明申请
    GENES FROM ACTINOBACILLUS SUCCINOGENES 13OZ (ATCC 55618) FOR PRODUCTION OF CHEMICALS FROM THE A. SUCCINOGENES C4-PATHWAY 审中-公开
    来自ACTINOBACILLUS SUCCINOGENES 13OZ的基因（ATCC 55618）用于从A.SCCC生产C4途径中的化学物质

    公开(公告)号：WO2007019301A3

    公开(公告)日：2007-07-12

    申请号：PCT/US2006030425

    申请日：2006-08-04

    Applicant: UNIV MICHIGAN STATE ,  ZEIKUS J GREGORY ,  MCKINLAY JAMES B ,  LAIVENIEKS MARIS ,  VIEILLE CLAIRE

    Inventor： ZEIKUS J GREGORY ,  MCKINLAY JAMES B ,  LAIVENIEKS MARIS ,  VIEILLE CLAIRE

    IPC: C12N1/20 ,  A61K39/02

    CPC classification number: C12P7/46 ,  C07K14/285 ,  C12P7/50 ,  C12P13/001 ,  C12P13/14 ,  C12P13/20

    Abstract: Actinobacillus succinogenes genes and methods of using the genes in genetically engineered A. succinogenes so as to improve production of chemicals such as succinate, fumarate, malate, 5-aminolevulinate, 2-oxoglutarate, glutamate, and aspartate. The genetically engineered A. succinogenes strains are capable of overexpressing C4 enzymes. The genetically engineered A. succinogenes can have one or more gene knockouts or modifications that inhibit C3 enzymes. The fluxes supplying substrate to the C4 pathway can also be improved in some of the genetically engineered A. succinogenes.

    Abstract translation: 放线芽胞杆菌属基因和使用基因工程改造的琥珀酸酵母中的基因的方法，以改善琥珀酸盐，富马酸盐，苹果酸盐，5-氨基乙酰丙酸盐，2-氧戊二酸盐，谷氨酸盐和天冬氨酸盐等化学品的生产。 遗传工程化的琥珀酸酵母菌株能够过量表达C4酶。 遗传工程化的琥珀酸酵母可以具有抑制C3酶的一种或多种基因敲除或修饰。 向C4途径提供底物的助熔剂也可以在一些遗传工程的A.succinogenes中得到改善。

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