利索-全球专利检索

  1. Login
  2. Sign Up

Search Results

8 records (took 0.01 seconds)

    MOLECULAR ARRAYS
    2.
    发明申请
    MOLECULAR ARRAYS 审中-公开
    分子阵列

    公开(公告)号：WO2010151714A2

    公开(公告)日：2010-12-29

    申请号：PCT/US2010039882

    申请日：2010-06-24

    Applicant: LIFE TECHNOLOGIES CORP ,  ZHOU ZHAOHUI ,  FRY GEORGE A ,  WOO SAM LEE

    Inventor： ZHOU ZHAOHUI ,  FRY GEORGE A ,  WOO SAM LEE

    IPC: C12Q1/68 ,  G01N33/50

    CPC classification number: C12Q1/6834 ,  C12Q2525/197

    Abstract: The disclosure relates generally to compositions, systems, and methods for use in preparing single molecule arrays, for example, arrays of single nucleic acid molecules, as well as arrays formed using such compositions, systems and methods by using a spacer which restricts the spacing between adjacent components of the array. In an exemplary embodiment, the array is formed by contacting a surface with a spacer-anchor complex including one or more spacers linked to an anchor, wherein the spacer comprises a linear or branched polymeric molecule folded to form a three-dimensional shape of desired size dimensions. The size of the folded spacer may optionally be adjusted to modulate the spacing distance between adjacent array components.

    Abstract translation: 本公开一般涉及用于制备单分子阵列的组合物，系统和方法，例如单个核酸分子的阵列，以及使用这种组合物形成的阵列，通过使用限制了间隔之间的间隔的系统和方法 阵列的相邻组件。 在一个示例性实施例中，阵列通过使表面与包括连接到锚的一个或多个间隔物的间隔物 - 锚定复合体接触而形成，其中间隔物包含折叠形成所需尺寸的三维形状的直链或支链聚合物分子 尺寸。 可以可选地调整折叠的间隔物的尺寸以调节相邻阵列部件之间的间隔距离。

    ASSAYS FOR S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES
    3.
    发明申请
    ASSAYS FOR S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES 审中-公开
    S-腺嘌呤依赖性甲基衍生物的测定

    公开(公告)号：WO2007087541A2

    公开(公告)日：2007-08-02

    申请号：PCT/US2007/060930

    申请日：2007-01-23

    Applicant: WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION ,  ZHOU, Zhaohui ,  DORGAN, Kathleen

    Inventor： ZHOU, Zhaohui ,  DORGAN, Kathleen

    IPC: C12Q1/52

    CPC classification number: C12Q1/48 ,  C12Q1/52 ,  G01N2333/91011

    Abstract: Disclosed are novel methyltransferase assay methods, comprising: including, in a reaction mixture for a methyltransferase activity, a purified or recombinant adenosine nucleosidase activity that catalyses release of an adenine or adenine derivative moiety from a transmethylation product, and a purified or recombinant adenine deaminase activity that catalyses deamination of the released moiety to hypoxanthine or respective derivative and ammonia, wherein the methyltransferase activity is rate-limiting; and determining the methyltransferase activity by spectrophotometric or chromatographic monitoring of the coupled deamination reaction products, or of subsequent enzymatic or chemical reactions coupled thereto. Coupled oxidation of the hypoxanthine to uric acid and hydrogen peroxide is optionally affected using purified or recombinant xanthine oxidase, wherein the methyltransferase activity is rate-limiting, and wherein determining the methyltransferase activity comprises monitoring of the coupled oxidation reaction. Variations are disclosed comprises monitoring of reaction products (e.g., to detect NH3, Hypoxanthine, H2O2, and Uric Acid).

    Abstract translation: 公开了新的甲基转移酶测定方法，其包括：在用于甲基转移酶活性的反应混合物中，包含催化腺嘌呤或腺嘌呤衍生物部分从转甲基化产物释放的纯化或重组腺苷核苷酶活性，以及​​纯化或重组腺嘌呤脱氨酶活性 催化释放的部分与次黄嘌呤或相应的衍生物和氨的脱氨基，其中甲基转移酶活性是速率限制的; 以及通过分光光度法或色谱监测偶联的脱氨基反应产物，或随后与其偶联的酶或化学反应来确定甲基转移酶活性。 次黄嘌呤与尿酸和过氧化氢的偶联氧化任选地使用纯化或重组的黄嘌呤氧化酶来影响，其中甲基转移酶活性是速率限制的，并且其中确定甲基转移酶活性包括监测偶联的氧化反应。 公开的变化包括监测反应产物（例如，检测NH 3，次黄嘌呤，H 2 O 2和尿酸）。

    MOLECULAR ARRAYS
    7.
    发明申请
    MOLECULAR ARRAYS 审中-公开

    公开(公告)号：WO2010151714A3

    公开(公告)日：2010-12-29

    申请号：PCT/US2010/039882

    申请日：2010-06-24

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  ZHOU, Zhaohui ,  FRY, George A. ,  WOO, Sam Lee

    Inventor： ZHOU, Zhaohui ,  FRY, George A. ,  WOO, Sam Lee

    IPC: C12Q1/68 ,  G01N33/50

    Abstract: The disclosure relates generally to compositions, systems, and methods for use in preparing single molecule arrays, for example, arrays of single nucleic acid molecules, as well as arrays formed using such compositions, systems and methods by using a spacer which restricts the spacing between adjacent components of the array. In an exemplary embodiment, the array is formed by contacting a surface with a spacer-anchor complex including one or more spacers linked to an anchor, wherein the spacer comprises a linear or branched polymeric molecule folded to form a three-dimensional shape of desired size dimensions. The size of the folded spacer may optionally be adjusted to modulate the spacing distance between adjacent array components.

    ASSAYS FOR S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES
    8.
    发明申请
    ASSAYS FOR S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES 审中-公开
    S-腺嘌呤依赖性甲基衍生物的测定

    公开(公告)号：WO2007087541A3

    公开(公告)日：2009-04-09

    申请号：PCT/US2007060930

    申请日：2007-01-23

    Applicant: UNIV WASHINGTON ,  ZHOU ZHAOHUI ,  DORGAN KATHLEEN

    Inventor： ZHOU ZHAOHUI ,  DORGAN KATHLEEN

    IPC: G01N21/62 ,  G01N30/02

    CPC classification number: C12Q1/48 ,  C12Q1/52 ,  G01N2333/91011

    Abstract: Disclosed are novel methyltransferase assay methods, comprising: including, in a reaction mixture for a methyltransferase activity, a purified or recombinant adenosine nucleosidase activity that catalyses release of an adenine or adenine derivative moiety from a transmethylation product, and a purified or recombinant adenine deaminase activity that catalyses deamination of the released moiety to hypoxanthine or respective derivative and ammonia, wherein the methyltransferase activity is rate-limiting; and determining the methyltransferase activity by spectrophotometric or chromatographic monitoring of the coupled deamination reaction products, or of subsequent enzymatic or chemical reactions coupled thereto. Coupled oxidation of the hypoxanthine to uric acid and hydrogen peroxide is optionally affected using purified or recombinant xanthine oxidase, wherein the methyltransferase activity is rate-limiting, and wherein determining the methyltransferase activity comprises monitoring of the coupled oxidation reaction. Variations are disclosed comprises monitoring of reaction products (e.g., to detect NH3, Hypoxanthine, H2O2, and Uric Acid).

    Abstract translation: 公开了新的甲基转移酶测定方法，其包括：在用于甲基转移酶活性的反应混合物中，包含催化腺嘌呤或腺嘌呤衍生物部分从转甲基化产物释放的纯化或重组腺苷核苷酶活性，以及​​纯化或重组腺嘌呤脱氨酶活性 催化释放的部分与次黄嘌呤或相应的衍生物和氨的脱氨，其中甲基转移酶活性是速率限制的; 以及通过分光光度法或色谱监测偶联的脱氨基反应产物，或随后与其偶联的酶或化学反应来确定甲基转移酶活性。 次黄嘌呤与尿酸和过氧化氢的偶联氧化任选地使用纯化或重组的黄嘌呤氧化酶来影响，其中甲基转移酶活性是速率限制的，并且其中确定甲基转移酶活性包括监测偶联的氧化反应。 公开的变化包括监测反应产物（例如，检测NH 3，次黄嘌呤，H 2 O 2和尿酸）。

Patent Agency Ranking