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    FLUORESCENT MOLECULAR ROTORS
    1.
    发明申请
    FLUORESCENT MOLECULAR ROTORS 审中-公开
    荧光分子转子

    公开(公告)号：WO2014051521A1

    公开(公告)日：2014-04-03

    申请号：PCT/SG2013/000420

    申请日：2013-09-26

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： BRENNER, Sydney ,  TEO, Yin Nah ,  GHADESSY, Farid ,  GOH, Leng Peng Walter ,  LEE, Min Yen

    IPC分类号： G01N33/48 ,  C07D219/08 ,  C07C255/41 ,  C07D277/64 ,  C07D213/24 ,  C07D495/04

    CPC分类号： G01N33/542 ,  C07C255/41 ,  C07C2603/50 ,  C07D219/08 ,  C07D401/14 ,  C07D417/06 ,  C07D455/04 ,  C07D495/04 ,  C07D519/00 ,  G01N33/582

    摘要： The present invention relates to methods and compositions for detecting an interaction between a protein and a ligand, comprising: (i) binding at least one fluorescent molecular rotor to said ligand or protein; and (ii) detecting a change in fluorescence emitted by said fluorescent molecular rotor after contact of the bound fluorescent molecular rotor with the other of said ligand or protein, thereby detecting an interaction between the ligand and the protein, wherein the fluorescent molecular rotor comprises: a rotating ?-bond; an electron-donating moiety; an electron-accepting moiety; and a ?-conjugated linker.

    摘要翻译： 本发明涉及用于检测蛋白质和配体之间相互作用的方法和组合物，其包括：（i）将至少一种荧光分子转子与所述配体或蛋白质结合; 和（ii）检测所述荧光分子转子在所述结合的荧光分子转子与所述配体或蛋白质中的另一个接触之后发射的荧光的变化，从而检测所述配体和所述蛋白质之间的相互作用，其中所述荧光分子转子包括： 旋转的？ 给电子部分; 电子接受部分; 和π共轭接头。

    A SIMPLE ONE-STEP REAL-TIME MOLECULAR SYSTEM FOR MICRORNA DETECTION
    3.
    发明申请
    A SIMPLE ONE-STEP REAL-TIME MOLECULAR SYSTEM FOR MICRORNA DETECTION 审中-公开
    一种简单的一步实时分子系统，用于微波检测

    公开(公告)号：WO2017146653A1

    公开(公告)日：2017-08-31

    申请号：PCT/SG2017/050086

    申请日：2017-02-24

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： AW, Sherry ,  TEO, Yin Nah ,  COHEN, Stephen Michael ,  TANG, Melissa Xue Mei

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q2600/178 ,  C12Q2525/161 ,  C12Q2525/207 ,  C12Q2565/133

    摘要： The present application discloses an isolated nucleic acid molecule for detecting the presence of a target nucleic acid sequence and uses thereof, wherein said molecule is capable of forming bimolecular interactions with a target nucleic acid sequence and forms a ternary complex stabilising a detection agent when bound. The detection agent is preferably DFHBI ((Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1 H -imidazol-5(4 H )-one. The target nucleic acid is preferably a miRNA sequence. Preferably, the isolated nucleic acid sequence comprises two domains that complementarily bind to the 5' and 3' ends of the target nucleic acid sequence. The isolated nucleic acid sequence also comprises regions of nucleic acids that do not bind with the target nucleic acid sequence and are capable of forming secondary structures, such as stems, stem loops, t-RNA type structures, cloverleaves, tetraloops and pseudoknots. The application further discloses corresponding methods of determining a disease in a subject by using the isolated nucleic acid sequence to detect one or more disease-specific target nucleic acids.

    摘要翻译： 本申请公开了用于检测靶核酸序列的存在的分离的核酸分子及其用途，其中所述分子能够与靶核酸序列形成双分子相互作用并形成三元 在结合时复合稳定检测剂。 检测剂优选为DFHBI（（Z）-4-（3,5-二氟-4-羟基亚苄基）-1,2-二甲基-1H-咪唑-5（4H） 目标核酸优选是miRNA序列，优选地，分离的核酸序列包含与靶核酸序列的5'和3'端互补结合的两个结构域，分离的核酸 序列还包含不与靶核酸序列结合并且能够形成二级结构（例如茎，茎环，t-RNA型结构，三叶草，四环和假结构）的核酸区域。本申请还公开了相应的方法 通过使用分离的核酸序列检测一种或多种疾病特异性靶核酸来确定受试者中的疾病。

    A Dimerizer Compound
    4.
    发明申请
    A Dimerizer Compound 审中-公开

    公开(公告)号：WO2015034440A3

    公开(公告)日：2015-03-12

    申请号：PCT/SG2014/000424

    申请日：2014-09-09

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： SEOW, Yiqi ,  WONG, Fong Tian ,  TEO, Yin Nah

    IPC分类号： C07D473/18 ,  C07D239/47

    摘要： There is provided a compound comprising at least two ligands that are individually coupled to a linker, wherein each ligand is independently selected from the group consisting of a substituted benzylguanine derivative, a substituted benzylcytosine derivative, a haloalkyl moiety, a drug, a peptide, a hormone, an inorganic compound and a protein. The compound may be part of an oligomer. The compound may be employed in a method of dimerizing a pair of proteins, which may alter a biological function in a cell. There is also provided a method of forming the compound.