公开(公告)号：WO2016196962A1

公开(公告)日：2016-12-08

申请号：PCT/US2016/035765

申请日：2016-06-03

Applicant: BIOAMBER INC.

Inventor： LAU, Man, Kit ,  HASS, Cathy, Staloch ,  AHMED, Hayat, Nuru ,  BAKER, Ralph, Thomas ,  MILLIS, James, R. ,  THAPA, Indira

IPC: C07K14/38 ,  C07K14/395 ,  C07K14/415 ,  C07K14/435 ,  C12N1/32

CPC classification number: C12N9/1096 ,  C07C51/377 ,  C07C51/38 ,  C07C59/147 ,  C07C59/185 ,  C07K14/38 ,  C07K14/395 ,  C12N1/32 ,  C12N9/1025 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P7/46 ,  C12P7/58 ,  C12P7/62 ,  C12Y203/03014 ,  C12Y206/01045 ,  C12Y301/0306 ,  C12Y402/01003

Abstract: The description relates to, inter alia, recombinant microorganisms, engineered metabolic pathways, chemical catalysts, and products produced through the use of the described methods and materials. The products produced include functionalized alpha substituted C4 dicarboxylic acids, as well as functionalized malonyl-CoA, malonic semialdehyde, and acrylic acids, and their salts, esters and lactones.

Abstract translation: 该描述尤其涉及重组微生物，工程化代谢途径，化学催化剂和通过使用所述方法和材料生产的产品。 产生的产物包括官能化的α取代的C4二羧酸，以及官能化的丙二酰辅酶A，丙二酸和丙烯酸及其盐，酯和内酯。

公开(公告)号：WO2014047407A1

公开(公告)日：2014-03-27

申请号：PCT/US2013/060853

申请日：2013-09-20

Applicant: BIOAMBER INC.

Inventor： LAU, Man, Kit ,  MERCOGLIANO, Christopher, P.

IPC: C12P7/40 ,  C12N15/52

CPC classification number: C12P13/001 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/005 ,  C12Y101/01 ,  C12Y103/01 ,  C12Y103/08 ,  C12Y206/01 ,  C12Y208/03 ,  C12Y301/02 ,  C12Y402/01

Abstract: Recombinant microorganisms comprising at least one exogenous nucleic acid sequence and capable of producing adipate semialdehyde are provided. Adipate semialdehyde may be produced in a synthesis pathway utilizing a single thiolase reaction. Adipate semialdehyde may also be produced from intermediates consisting of alpha, omega difunctional aliphatic organic molecules. Methods of using recombinant microorganisms to produce 6-aminocaproic acid, adipic acid, hexamethylenediamine and 1.6- hexanediol are also provided.

Abstract translation: 提供了包含至少一种外源核酸序列并能够产生己二酸半醛的重组微生物。 己二酸半醛可以通过利用单一硫解酶反应的合成途径产生。 己二酸半醛也可以由由α，ω-二官能脂族有机分子组成的中间体产生。 还提供了使用重组微生物生产6-氨基己酸，己二酸，六亚甲基二胺和1.6-己二醇的方法。

公开(公告)号：WO2013163292A2

公开(公告)日：2013-10-31

申请号：PCT/US2013/037985

申请日：2013-04-24

Applicant: BIOAMBER INC.

Inventor： LAU, Man, Kit

IPC: C12N1/21

CPC classification number: C12P7/44 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42

Abstract: A method of increasing the production a difunctional alkane in a microorganism that produces a difunctional alkane from alpha-keto acid by increasing the production of homocitrate in the cell relative to a wild-type or parent cell. The production of homocitrate may be obtained by engineering pathways that increase the production of alpha-ketoacid, such as alpha-ketoglutarate.

Abstract translation: 通过相对于野生型或亲本细胞增加细胞中高柠檬酸盐的产生，增加微生物中生成双官能烷烃从α-酮酸产生的方法。 高倍硝酸盐的生产可以通过增加α-酮酸生产的工程途径获得，例如α-酮戊二酸。

公开(公告)号：WO2014043182A2

公开(公告)日：2014-03-20

申请号：PCT/US2013/059170

申请日：2013-09-11

Applicant: BIOAMBER INC.

Inventor： MILLIS, James, R. ,  LAU, Man, Kit

IPC: C12P7/44

CPC classification number: C07C51/38 ,  C07C51/347 ,  C07C51/36 ,  C07C51/373 ,  C07C51/377 ,  C12P7/44 ,  C12P7/48 ,  C12P7/50 ,  C07C59/245 ,  C07C55/14

Abstract: Processes process for producing adipate or adipic acid using biological pathways and chemical catalyzes are disclosed. Homocitric acid may be a substrate in reaction pathways leading to adipic acid or a salt thereof.

Abstract translation: 公开了使用生物学途径和化学催化剂生产己二酸或己二酸的方法。 异柠檬酸可以是导致己二酸或其盐的反应途径中的底物。