公开(公告)号：WO2016033246A1

公开(公告)日：2016-03-03

申请号：PCT/US2015/047046

申请日：2015-08-26

Applicant: CARIBOU BIOSCIENCES, INC.

Inventor： CAMERON, Peter, Sean ,  HAURWITZ, Rachel, E. ,  MAY, Andrew, P. ,  NYE, Christopher, H ,  VAN OVERBEEK, Megan

IPC: C12N15/11 ,  C12N15/09

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12Y301/30

Abstract: Methods for use with Type II CRISPR-Cas9 systems for increasing Cas9-mediated genome engineering efficiency are disclosed. The methods can be used to decrease the number of off-target nucleic acid double-stranded breaks and/or to enhance homology-directed repair of a cleaved target nucleic acid.

Abstract translation: 公开了与II型CRISPR-Cas9系统一起用于增加Cas9介导的基因组工程效率的方法。 所述方法可用于减少脱靶核酸双链断裂的数目和/或增强切割的靶核酸的同源性修复。

公开(公告)号：WO2014189628A1

公开(公告)日：2014-11-27

申请号：PCT/US2014/033885

申请日：2014-04-11

Applicant: CARIBOU BIOSCIENCES, INC.

Inventor： VAN DER OOST, John ,  SWARTS, Daniël, Christianus ,  MAY, Andrew, Paul ,  HAURWITZ, Rachel, E.

IPC: C12Q1/00

CPC classification number: C12N15/902 ,  C12N15/10 ,  C12N15/102 ,  G01N33/542

Abstract: This disclosure provides for compositions and methods for the use of designed nucleic acid-targeting nucleic acids, Argonautes, and complexes thereof. In one aspect, the disclosure provides for a composition comprising: a complex comprising: an Argonaute and a designed nucleic acid-targeting nucleic acid; and a target nucleic acid, wherein the designed nucleic acid-targeting nucleic acid is hybridized to the target nucleic acid. In some embodiments, the target nucleic acid is double-stranded. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a prokaryotic Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a bacterial Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an archaeal Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a mesophile. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a thermophile.

Abstract translation: 本公开提供了使用设计的核酸靶向核酸Argonautes及其复合物的组合物和方法。 一方面，本公开内容提供了一种组合物，其包含：复合物，其包含：Argonaute和设计的核酸靶向核酸; 和靶核酸，其中设计的核酸靶向核酸与靶核酸杂交。 在一些实施方案中，靶核酸是双链的。 在一些实施方案中，Argonaute与原核Argonaute包含至少30％的氨基酸同一性。 在一些实施方案中，Argonaute与细菌Argonaute包含至少30％的氨基酸同一性。 在一些实施方案中，Argonaute与古细菌Argonaute包含至少30％的氨基酸同一性。 在一些实施方案中，Argonaute与来自嗜中性粒细胞的Argonaute包含至少30％的氨基酸同一性。 在一些实施方案中，Argonaute包含与嗜热菌的Argonaute至少30％的氨基酸同一性。

公开(公告)号：WO2014150624A1

公开(公告)日：2014-09-25

申请号：PCT/US2014/023828

申请日：2014-03-12

Applicant: CARIBOU BIOSCIENCES, INC.

Inventor： MAY, Andrew, Paul ,  HAURWITZ, Rachel, E. ,  DOUDNA, Jennifer, A. ,  BERGER, James, M. ,  CARTER, Matthew, Merrill ,  DONOHOUE, Paul

IPC: C12Q1/68

CPC classification number: C12N15/11 ,  A61K38/465 ,  A61K47/549 ,  A61K47/6455 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/52 ,  C12N15/85 ,  C12N15/90 ,  C12N15/902 ,  C12N15/907 ,  C12N2310/20 ,  C12N2310/531 ,  C12N2310/533 ,  C12N2800/80 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/203

Abstract: This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof. Genome engineering can refer to altering the genome by deleting, inserting, mutating, or substituting specific nucleic acid sequences. The altering can be gene or location specific. Genome engineering can use nucleases to cut a nucleic acid thereby generating a site for the alteration. Engineering of non-genomic nucleic acid is also contemplated.

Abstract translation: 本公开提供了使用核酸靶向核酸及其复合物的组合物和方法。 基因组工程可以指通过删除，插入，突变或代替特定核酸序列来改变基因组。 改变可以是基因或位置特异性。 基因组工程可以使用核酸酶切割核酸，从而产生改变的位点。 还设想了非基因组核酸的工程。