公开(公告)号：WO2013173394A2

公开(公告)日：2013-11-21

申请号：PCT/US2013/041031

申请日：2013-05-14

Applicant: CB BIOTECHNOLOGIES, INC.

Inventor： WANG, Chunlin ,  HAN, Jian

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6851 ,  C12Q1/6869 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2527/101 ,  C12Q2535/122 ,  C12Q2539/107 ,  C12Q2563/179

Abstract: Disclosed is a method for improving the sensitivity and accuracy of quantitative detection of polynucleotides in a sample, such a clinical specimen, by a method that utilizes a two- or three-step process of tagging/labeling target molecules and adding an adapter sequence for adding a universal primer for efficient amplification of targets while decreasing target amplification bias. When combined with the step of statistically correcting for sequencing errors, the method can significantly increase the accuracy of quantitative detection of polynucleotides in a sample.

Abstract translation: 公开了通过利用标记/标记靶分子的两步或三步法并添加用于添加的衔接子序列的方法来提高样品（例如临床样品）中多核苷酸的定量检测的灵敏度和准确度的方法 一种通用引物，用于有效放大靶标，同时降低靶标扩增偏倚。 当与统计校正测序误差的步骤结合时，该方法可以显着提高样品中多核苷酸的定量检测的准确性。

公开(公告)号：WO2014047646A9

公开(公告)日：2014-03-27

申请号：PCT/US2013/061460

申请日：2013-09-24

Applicant: CB BIOTECHNOLOGIES, INC.

Inventor： HAN, Jian ,  WANG, Chunlin

IPC: C12P19/34 ,  C07H21/04

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non- canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

公开(公告)号：WO2014124451A1

公开(公告)日：2014-08-14

申请号：PCT/US2014/015841

申请日：2014-02-11

Applicant: CB BIOTECHNOLOGIES, INC.

Inventor： WANG, Chunlin ,  HAN, Jian

IPC: C12Q1/68

CPC classification number: G06F19/22 ,  C12Q1/6869 ,  C12Q1/6883 ,  C40B30/02 ,  G06F19/24 ,  G06F19/28

Abstract: Disclosed is a method for amplifying RNA from T and B-cell populations and using the amplified RNA products to evaluate the possible correlation between a normal or abnormal immune response and the development of a disease such as an autoimmune disease, cancer, diabetes, or heart disease.

Abstract translation: 公开了一种从T和B细胞群体中扩增RNA的方法，并使用扩增的RNA产物来评估正常或异常免疫应答与疾病如自身免疫疾病，癌症，糖尿病或心脏发育之间可能的相关性 疾病。

公开(公告)号：WO2014047646A1

公开(公告)日：2014-03-27

申请号：PCT/US2013/061460

申请日：2013-09-24

Applicant: CB BIOTECHNOLOGIES, INC.

Inventor： HAN, Jian ,  WANG, Chunlin

IPC: C12P19/34 ,  C07H21/04

CPC classification number: C12Q1/6881 ,  C12Q1/6869 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2565/301

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non- canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

Abstract translation: 本公开通常涉及用于分析样品的多重方法，其包括使用多核苷酸扩增产生扩增产物，其中一个或多个靶序列用非干扰的，非抵消的靶特异性多核苷酸鉴定标签进行标记，通过所述扩增产物 非取消靶特异性多核苷酸鉴定标签序列，以检测一个或多个特异性多核苷酸鉴定标签的存在。 特定多核苷酸识别标签的存在与特定靶序列的存在相关。