-
公开(公告)号：WO2016144776A1
公开(公告)日：2016-09-15
申请号：PCT/US2016/020939
申请日：2016-03-04
Applicant: CB BIOTECHNOLOGIES, INC.
Inventor： HAN, Jian
CPC classification number: G06F19/20
Abstract: The present invention pertains to methods for determining the change in the immune repertoire of an individual suffering from disease or undergoing therapy for the treatment of a disease. The methods use the difference between the change in the level of immune cell diversity seen in an individual before, during or after a health event to determine the effect of the disease, or the effect of a treatment regime, on the individual. The difference in the level of immune cell diversity is referred to as the Delta Index and is defined as the quantitative and qualitative change (gain or loss of clones) of an individual's most dynamic immune cells over time.
Abstract translation: 本发明涉及用于确定患有疾病或正在接受治疗疾病的个体的免疫谱的变化的方法。 该方法使用在健康事件之前，期间或之后在个体中观察到的免疫细胞多样性水平的变化之间的差异，以确定疾病的效果或治疗方案对个体的影响。 免疫细胞多样性水平的差异被称为三角洲指数，并被定义为个体最具动态免疫细胞随时间的定量和定性变化（克隆的增益或损失）。
-
2.发明申请
公开(公告)号：WO2013173394A2
公开(公告)日：2013-11-21
申请号：PCT/US2013/041031
申请日：2013-05-14
Applicant: CB BIOTECHNOLOGIES, INC.
Inventor： WANG, Chunlin , HAN, Jian
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6809 , C12Q1/6851 , C12Q1/6869 , C12Q2521/107 , C12Q2525/155 , C12Q2525/161 , C12Q2525/191 , C12Q2527/101 , C12Q2535/122 , C12Q2539/107 , C12Q2563/179
Abstract: Disclosed is a method for improving the sensitivity and accuracy of quantitative detection of polynucleotides in a sample, such a clinical specimen, by a method that utilizes a two- or three-step process of tagging/labeling target molecules and adding an adapter sequence for adding a universal primer for efficient amplification of targets while decreasing target amplification bias. When combined with the step of statistically correcting for sequencing errors, the method can significantly increase the accuracy of quantitative detection of polynucleotides in a sample.
Abstract translation: 公开了通过利用标记/标记靶分子的两步或三步法并添加用于添加的衔接子序列的方法来提高样品（例如临床样品）中多核苷酸的定量检测的灵敏度和准确度的方法 一种通用引物，用于有效放大靶标，同时降低靶标扩增偏倚。 当与统计校正测序误差的步骤结合时，该方法可以显着提高样品中多核苷酸的定量检测的准确性。
-
3.发明申请IDENTIFICATION OF ANTIGEN-SPECIFIC ADAPTIVE IMMUNE RESPONSES USING ARM-PCR AND HIGH-THROUGHPUT SEQUENCING 审中-公开使用ARM-PCR和高通量序列鉴定抗原特异性自适应免疫应答
公开(公告)号：WO2013049727A1
公开(公告)日：2013-04-04
申请号：PCT/US2012/058128
申请日：2012-09-28
Applicant: CB BIOTECHNOLOGIES, INC. , HAN, Jian , BYRNE-STEELE, Miranda
Inventor： HAN, Jian , BYRNE-STEELE, Miranda
CPC classification number: G01N33/53 , C07K16/00 , C07K16/1018 , C07K2317/21 , C07K2317/55 , C12Q1/6874 , G01N33/56966
Abstract: Disclosed is a method for correlating at least one amino acid sequence from an antibody isolated from human or animal blood with at least one DNA sequence corresponding to the antibody in the immunorepertoire of the human or animal. The method also provides a means for pairing heavy and light chains to produce synthesized monoclonal antibodies.
Abstract translation: 公开了将人或动物血液中分离的抗体的至少一个氨基酸序列与对应于人或动物的免疫曲线中的抗体的至少一个DNA序列相关联的方法。 该方法还提供了用于配对重链和轻链以产生合成的单克隆抗体的方法。
-
公开(公告)号：WO2012097374A1
公开(公告)日：2012-07-19
申请号：PCT/US2012/021594
申请日：2012-01-17
Applicant: CB BIOTECHNOLOGIES, INC. , HAN, Jian
Inventor： HAN, Jian
CPC classification number: G01N33/56972 , C12Q1/686 , C12Q1/6869 , C12Q2525/155 , C12Q2535/122 , C12Q2537/165 , C12Q2549/119
Abstract: Disclosed is a method for distinguishing the immunorepertoires of normal, healthy individuals from those of individuals who have symptomatic and/or non-symptomatic disease.
Abstract translation: 公开了一种区分正常健康个体与有症状和/或无症状疾病个体免疫缺陷的方法。
-
5.发明申请
公开(公告)号：WO2014047646A9
公开(公告)日：2014-03-27
申请号：PCT/US2013/061460
申请日：2013-09-24
Applicant: CB BIOTECHNOLOGIES, INC.
Inventor： HAN, Jian , WANG, Chunlin
Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non- canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.
-
Patent Agency Ranking6.发明申请
公开(公告)号：WO2013173394A3
公开(公告)日：2014-01-23
申请号：PCT/US2013041031
申请日：2013-05-14
Applicant: CB BIOTECHNOLOGIES INC
Inventor： WANG CHUNLIN , HAN JIAN
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6809 , C12Q1/6851 , C12Q1/6869 , C12Q2521/107 , C12Q2525/155 , C12Q2525/161 , C12Q2525/191 , C12Q2527/101 , C12Q2535/122 , C12Q2539/107 , C12Q2563/179
Abstract: Disclosed is a method for improving the sensitivity and accuracy of quantitative detection of polynucleotides in a sample, such a clinical specimen, by a method that utilizes a two- or three-step process of tagging/labeling target molecules and adding an adapter sequence for adding a universal primer for efficient amplification of targets while decreasing target amplification bias. When combined with the step of statistically correcting for sequencing errors, the method can significantly increase the accuracy of quantitative detection of polynucleotides in a sample.
Abstract translation: 公开了通过利用标记/标记靶分子的两步或三步法并添加用于添加的衔接子序列的方法来提高样品（例如临床样品）中多核苷酸的定量检测的灵敏度和准确度的方法 一种通用引物，用于有效放大靶标，同时降低靶标扩增偏倚。 当与统计校正测序误差的步骤结合时，该方法可以显着提高样品中多核苷酸的定量检测的准确性。
-
7.发明申请METHOD FOR IDENTIFYING DISEASE-ASSOCIATED CDR3 PATTERNS IN AN IMMUNE REPERTOIRE 审中-公开用于识别免疫球蛋白相关CDR3模式的方法
公开(公告)号：WO2016144996A1
公开(公告)日：2016-09-15
申请号：PCT/US2016/021430
申请日：2016-03-09
Applicant: CB BIOTECHNOLOGIES, INC.
Inventor： HAN, Jian
CPC classification number: C12Q1/6883 , C12Q1/6881 , C12Q2600/156 , C12Q2600/172 , G01N33/6854 , G06F19/18
Abstract: The present disclosure generally pertains to a method for developing diagnostic tests that are based on the immune response and the resulting immune repertoire. The presently disclosed method increases the signal and reduces the background to allow the identification of shared CDR3s that can be used to produce a disease signature. The presently disclosed method may be used to develop a diagnostic test for different diseases including, but not limited to, cancer, autoimmune disease, inflammatory disease and infectious disease.
Abstract translation: 本公开通常涉及用于开发基于免疫应答和所得免疫谱的诊断测试的方法。 目前公开的方法增加了信号并减少了背景以允许识别可用于产生疾病特征的共享CDR3。 本公开的方法可用于开发不同疾病的诊断测试，包括但不限于癌症，自身免疫性疾病，炎性疾病和感染性疾病。
-
公开(公告)号：WO2014124451A1
公开(公告)日：2014-08-14
申请号：PCT/US2014/015841
申请日：2014-02-11
Applicant: CB BIOTECHNOLOGIES, INC.
Inventor： WANG, Chunlin , HAN, Jian
IPC: C12Q1/68
CPC classification number: G06F19/22 , C12Q1/6869 , C12Q1/6883 , C40B30/02 , G06F19/24 , G06F19/28
Abstract: Disclosed is a method for amplifying RNA from T and B-cell populations and using the amplified RNA products to evaluate the possible correlation between a normal or abnormal immune response and the development of a disease such as an autoimmune disease, cancer, diabetes, or heart disease.
Abstract translation: 公开了一种从T和B细胞群体中扩增RNA的方法，并使用扩增的RNA产物来评估正常或异常免疫应答与疾病如自身免疫疾病，癌症，糖尿病或心脏发育之间可能的相关性 疾病。
-
9.发明申请MULTIPLEX PYROSEQUENCING USING NON-INTERFERING NOISE CANCELLING POLYNUCLEOTIDE IDENTIFICATION TAGS 审中-公开使用非干扰噪声消除多核苷酸鉴定标签进行多重选择
公开(公告)号：WO2014047646A1
公开(公告)日：2014-03-27
申请号：PCT/US2013/061460
申请日：2013-09-24
Applicant: CB BIOTECHNOLOGIES, INC.
Inventor： HAN, Jian , WANG, Chunlin
CPC classification number: C12Q1/6881 , C12Q1/6869 , C12Q2531/113 , C12Q2537/143 , C12Q2565/301
Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non- canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.
Abstract translation: 本公开通常涉及用于分析样品的多重方法，其包括使用多核苷酸扩增产生扩增产物，其中一个或多个靶序列用非干扰的，非抵消的靶特异性多核苷酸鉴定标签进行标记，通过所述扩增产物 非取消靶特异性多核苷酸鉴定标签序列，以检测一个或多个特异性多核苷酸鉴定标签的存在。 特定多核苷酸识别标签的存在与特定靶序列的存在相关。
-
公开(公告)号：WO2012100089A1
公开(公告)日：2012-07-26
申请号：PCT/US2012/021917
申请日：2012-01-19
Applicant: CB BIOTECHNOLOGIES, INC. , HAN, Jian
Inventor： HAN, Jian
Abstract: Disclosed is a method for calculating a Polymerase Preference Index (PPI) for potential primers for DNA sequencing and/or amplification, and thereby increasing the efficiency of DNA sequencing and/or amplification performed using primers selected according to their PPI number.
Abstract translation: 公开了一种用于计算用于DNA测序和/或扩增的潜在引物的聚合酶偏好指数（PPI）的方法，从而使用根据其PPI数选择的引物进行DNA测序和/或扩增的效率。
-
-
-
-
-
-
-
-
-
Search Results
10
records
(took 0.007 seconds)
