公开(公告)号：WO2006060513A2

公开(公告)日：2006-06-08

申请号：PCT/US2005/043373

申请日：2005-11-30

Applicant: CENTOCOR, INC. ,  CARTON, Jill ,  CUNNINGHAM, Mark ,  DAS, Anuk ,  DUFFY, Karen ,  KNIGHT, David, M. ,  LAMB, Roberta ,  MBOW, Mouhamadou, L. ,  RAGHUNATHAN, Gopalan ,  SAN MATEO, Lani ,  SARISKY, Robert, T. ,  STOWELL, Nicole ,  SWEET, Raymond ,  ZHAO, Shanrong ,  CHEN, Shizhong ,  GILES-KOMAR, Jill ,  PICHA, Kristen ,  STOJANOVIC-SUSULIC, Vedrana ,  GOLETZ, Theresa, J.

Inventor： CARTON, Jill ,  CUNNINGHAM, Mark ,  DAS, Anuk ,  DUFFY, Karen ,  KNIGHT, David, M. ,  LAMB, Roberta ,  MBOW, Mouhamadou, L. ,  RAGHUNATHAN, Gopalan ,  SAN MATEO, Lani ,  SARISKY, Robert, T. ,  STOWELL, Nicole ,  SWEET, Raymond ,  ZHAO, Shanrong ,  CHEN, Shizhong ,  GILES-KOMAR, Jill ,  PICHA, Kristen ,  STOJANOVIC-SUSULIC, Vedrana ,  GOLETZ, Theresa, J.

IPC: A61K39/395 ,  C07K16/28

CPC classification number: C07K16/2896 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92

Abstract: Toll Like Receptor 3 (TLR3) antagonists, polynucleotides encoding TLR3 antagonists or fragments thereof, and methods of making and using the foregoing are disclosed.

Abstract translation: Toll样受体3（TLR3）拮抗剂，编码TLR3拮抗剂或其片段的多核苷酸，以及制备和使用前述物质的方法。

公开(公告)号：WO2008052108A2

公开(公告)日：2008-05-02

申请号：PCT/US2007/082516

申请日：2007-10-25

Applicant: CENTOCOR, INC. ,  CHEN, Shizhong ,  ZHAO, Shanrong

Inventor： CHEN, Shizhong ,  ZHAO, Shanrong

IPC: C40B40/10

CPC classification number: C40B40/10 ,  C07K16/2896 ,  C07K16/465 ,  C12N15/1089 ,  C40B30/04

Abstract: Methods useful for human adapting non-human monoclonal antibodies are disclosed. The methods select candidate human antibody framework sequences from a human germline framework database.

Abstract translation: 公开了可用于人类适应非人单克隆抗体的方法。 这些方法从人种系框架数据库中选择候选人抗体框架序列。

公开(公告)号：WO2009111336A2

公开(公告)日：2009-09-11

申请号：PCT/US2009/035562

申请日：2009-02-27

Applicant: CHEN, Shizhong

Inventor： CHEN, Shizhong

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  B01L3/50255 ,  B01L3/563 ,  B01L2200/0689 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0409

Abstract: Described herein are novel methods to purify plasmid DNA from host cells. After a standard lysis procedure that releases plasmid DNA from the host cells, two sequential precipitation procedures separate plasmid DNA from essentially all impurities of the host cells. An optional third precipitation procedure further polishes the plasmid DNA for its most demanding applications, such as for gene therapy or pharmaceutical uses.

Abstract translation: 本文描述了从宿主细胞中纯化质粒DNA的新方法。 在从宿主细胞释放质粒DNA的标准裂解程序之后，两个连续的沉淀程序将质粒DNA与宿主细胞的基本上所有的杂质分离。 任选的第三沉淀方法进一步抛光质粒DNA用于其最苛刻的应用，例如用于基因治疗或药物用途。