中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

181 records (took 0.029 seconds)

    METHODS AND SYSTEM FOR GENERATING AND COMPARING REDUCED GENOME DATA SETS
    1.
    发明申请
    METHODS AND SYSTEM FOR GENERATING AND COMPARING REDUCED GENOME DATA SETS 审中-公开
    用于生成和比较减少的基因组数据集的方法和系统

    公开(公告)号：WO2017210102A1

    公开(公告)日：2017-12-07

    申请号：PCT/US2017/034625

    申请日：2017-05-26

    Applicant: INSTITUTE FOR SYSTEMS BIOLOGY

    Inventor： GLUSMAN, Gustavo ,  ROBINSON, Robert, Maxwell

    IPC: C12Q1/68 ,  C40B20/00 ,  C40B40/06 ,  G06F19/10 ,  G06F19/22

    CPC classification number: C12N15/1089 ,  C12Q1/68 ,  C12Q1/6827 ,  C40B20/00 ,  C40B40/06 ,  G16B20/20 ,  G16B30/00 ,  G16B35/10 ,  G16B35/20 ,  G16B45/00 ,  G16B50/40

    Abstract: An ultra-fast solution to the problem of comparing genomes across sequencing technologies and genome freezes, while preserving privacy, is presented. A method for transforming a standard genome representation (i.e., a list of variants relative to a reference) into a "fingerprint" of the genome does not require knowledge of the technology, reference and encoding used, and yields fingerprints that can be readily compared to ascertain relatedness between two genome representations. Because of their reduced size, computation on the genome fingerprints is fast and requires little memory. This enables scaling up a variety of important genome analyses, including determinations of degree of relatedness, recognizing duplicative sequenced genomes in a set, and many others. Because the original genome representation cannot be reconstructed from its fingerprint, the method also has significant implications for privacy-preserving genome analytics.

    Abstract translation: 本文介绍了一种快速解决跨测序技术和基因组冻结的基因组比较问题，同时保护隐私的超快速解决方案。 用于将标准基因组表示（即，相对于参考的变体列表）转换为“指纹” 的基因组不需要关于所使用的技术，参考和编码的知识，并且产生可以容易地比较的指纹以确定两个基因组表示之间的相关性。 由于它们的尺寸减小，基因组指纹图谱的计算速度很快并且只需很少的记忆。 这使得可以扩大各种重要的基因组分析，包括确定亲缘关系程度，识别一组中重复测序的基因组以及许多其他基因组。 由于原始基因组表达无法通过其指纹重建，因此该方法对保护隐私的基因组分析也具有重要意义。

    코돈 조합화 및 변이유발을 이용한 유전자 라이브러리의 합성 방법
    4.
    发明申请
    코돈 조합화 및 변이유발을 이용한 유전자 라이브러리의 합성 방법 审中-公开
    使用CODON组合和MUTAGENESIS合成基因库的方法

    公开(公告)号：WO2014092458A1

    公开(公告)日：2014-06-19

    申请号：PCT/KR2013/011492

    申请日：2013-12-11

    Applicant: 연세대학교 산학협력단

    Inventor： 방두희 ,  박상언 ,  이준구 ,  이지원

    IPC: C12Q1/68 ,  C40B40/08 ,  C12N15/11

    CPC classification number: C12N15/102 ,  C12N15/1089 ,  C12N15/1093 ,  C12N15/67 ,  C12Q2563/179

    Abstract: 같은 단백질로 번역되는 3개의 핵산 염기서열(코돈)의 조합을 이용함으로써 유전자 라이브러리 합성 후, 핵산 염기서열의 다양한 라이브러리 서열의 분석 시 쉽게 에러를 찾아낼 수 있는 방식을 제안한다. 이를 이용해 단백질 서열은 같지만 핵산 염기서열은 서로 다른 유전자 라이브러리를 만들 수 있다는 것을 보여준다. 이는 생체 내에서 특정 유전자를 발현시키기 위해 최적화되어 있는 코돈의 사용을 변화시킴으로써 코돈 변화에 따른 유전자 발현의 상관관계를 측정할 수 있는 새로운 실험방법을 제공한다. 또한, 같은 방식으로 단백질 서열 역시 일부 유사단백질 서열로 변환시킨 유전자 라이브러리를 동시에 합성 및 분석할 수 있으며, 이를 통해 발현된 유전자의 일부 단백질 서열의 변화가 해당 유전자의 기능에 어떠한 영향을 미치는지 확인할 수 있는 방법을 제공한다.

    Abstract translation: 提出了一种能够在通过使用翻译成相同蛋白质的三个核酸碱基序列（密码子）的组合合成基因文库之后在分析核酸碱基序列的各种文库序列期间容易地发现错误的方法。 这表明可以产生具有相同蛋白质序列但不同核酸碱基序列的基因文库。 本发明提供了一种新的测试方法，其能够通过改变在体内表达特定基因而优化的密码子的使用来测量根据密码子变化的基因表达之间的相关性。 同样地，本发明同样地提供能够同时合成和分析其中蛋白质序列被相似蛋白质序列改变的基因文库的方法，因此能够鉴别部分蛋白质序列的变化的影响 的表达基因具有相应基因的功能。

    COMPOSITIONS AND METHODS FOR HIGH FIDELITY ASSEMBLY OF NUCLEIC ACIDS
    6.
    发明申请
    COMPOSITIONS AND METHODS FOR HIGH FIDELITY ASSEMBLY OF NUCLEIC ACIDS 审中-公开
    用于高浓度组装的核酸的组合物和方法

    公开(公告)号：WO2013032850A2

    公开(公告)日：2013-03-07

    申请号：PCT/US2012/052036

    申请日：2012-08-23

    Applicant: GEN9, INC. ,  JACOBSON, Joseph ,  SCHINDLER, Daniel ,  LAWTON, Scott S.

    Inventor： JACOBSON, Joseph ,  SCHINDLER, Daniel ,  LAWTON, Scott S.

    IPC: C12N15/10 ,  C12N15/66 ,  C12P19/34 ,  C40B50/06 ,  G06F19/10

    CPC classification number: C12N15/66 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/1031 ,  C12N15/1089 ,  C12P19/34 ,  C12Q2521/301 ,  C12Q2521/501 ,  G06F19/22

    Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid. In certain embodiments, the plurality of blunt-end double-stranded nucleic acid fragments can be provided by: releasing a plurality of oligonucleotides synthesized on a solid support; and synthesizing complementary strands of the plurality of oligonucleotides using a polymerase based reaction.

    Abstract translation: 本发明的方面涉及用于设计和产生靶核酸的方法，组合物和算法。 该方法可以包括：（1）提供多个在其两端具有限制酶识别序列的平末端双链核酸片段; （2）通过酶消化产生多个具有两个不同和非互补突出端的粘性末端双链核酸片段; （3）用连接酶连接多个内聚端双链核酸片段; 和（4）形成多个内聚端双链核酸片段的线性排列，其中独特的排列包含靶核酸。 在某些实施方案中，多个平末端双链核酸片段可以通过以下方式提供：释放在固体支持物上合成的多种寡核苷酸; 并使用基于聚合酶的反应合成多个寡核苷酸的互补链。

    ANCESTRAL PROTEINS
    7.
    发明申请
    ANCESTRAL PROTEINS 审中-公开
    前列腺蛋白

    公开(公告)号：WO2012009693A1

    公开(公告)日：2012-01-19

    申请号：PCT/US2011/044275

    申请日：2011-07-15

    Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK ,  GEORGIA TECH RESEARCH CORPORATION ,  FERNANDEZ, Julio, M. ,  PEREZ-JIMENEZ, Raul ,  GAUCHER, Eric ,  KOSURI, Pallav

    Inventor： FERNANDEZ, Julio, M. ,  PEREZ-JIMENEZ, Raul ,  GAUCHER, Eric ,  KOSURI, Pallav

    IPC: C07K14/195

    CPC classification number: C12N9/0036 ,  C07K2299/00 ,  C12N15/1089

    Abstract: The invention provides a method for increasing the stability and/or activity of a polypeptide at low pH and/or elevated temperatures. The invention further provides a method for increasing the melting termperature of a polypeptide. Also provided are paleoenzymologically reconstructed thioredoxin polypeptides having activity at higher temperatures and/or lower pH than extant thioredoxin polypepetides, as well as paleoenzymologically reconstructed thioredoxin polypeptides having higher melting temperatures than extant thioredoxin polypepetides.

    Abstract translation: 本发明提供了在低pH和/或升高的温度下增加多肽的稳定性和/或活性的方法。 本发明进一步提供了增加多肽的熔解温度的方法。 还提供了具有比现有的硫氧还蛋白多肽具有更高的温度和/或更低的pH的活性的古生物学重建的硫氧还蛋白多肽，以及具有比现有的硫氧还蛋白多肽具有更高的熔化温度的古氧化重建的硫氧还蛋白多肽。

Patent Agency Ranking