公开(公告)号：WO2005100424A3

公开(公告)日：2006-01-26

申请号：PCT/FR2005000573

申请日：2005-03-10

Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  SUDOR JAN ,  BARBIER VALESSA

Inventor： SUDOR JAN ,  BARBIER VALESSA

IPC: C08F265/10 ,  C07K17/08 ,  C08F220/52 ,  C08J5/12 ,  C12N11/08 ,  C12Q1/68 ,  G01N33/545

CPC classification number: C08F265/10

Abstract: The invention relates to water-soluble N,N-dialkylacrylamide-based high molecular weight random polymers and monomers laterally functionalised by molecules of interest and to the use thereof for preparing solid supports provided with at least one surface on which said polymers are adsorbed. Said invention also relates to the thus modified solid supports, to a method for the production thereof and to different uses thereof, in particular for immobilising molecules of interest or for carrying out chemical, biochemical or biological reactions. .

Abstract translation: 本发明涉及水溶性N，N-二烷基丙烯酰胺基高分子量无规聚合物和由目标分子横向功能化的单体，以及其用于制备固体载体的用途，所述固体载体具有吸附所述聚合物的至少一个表面。 所述发明还涉及如此改性的固体支持物，其生产方法及其不同用途，特别是用于固定感兴趣的分子或用于进行化学，生化或生物反应。 。

公开(公告)号：WO2007003756A3

公开(公告)日：2007-03-01

申请号：PCT/FR2006001496

申请日：2006-06-28

Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  CENTRE NAT RECH SCIENT ,  SUDOR JAN ,  VINET FRANCOISE ,  GUE ANNE-MARIE ,  ESTEVE DANIEL

Inventor： SUDOR JAN ,  VINET FRANCOISE ,  GUE ANNE-MARIE ,  ESTEVE DANIEL

IPC: G01N27/447

CPC classification number: C03C17/3405 ,  C03C17/004 ,  C03C2218/11 ,  C03C2218/31 ,  G01N27/44752

Abstract: The invention concerns the field of microfluidics and in particular that of electrophoresis on a micro- or nanofluidic device. More particularly, the invention concerns the use of a reactive polymer coating adapted to be submitted to a phase separation under the influence of an external stimulation of chemical or physical type, to modulate the electrokinetic flows (electroosmotic or eletrophoretic flows during electrophoresis for example) in a micro- or nanofluidic device. The invention also concerns a method for varying the electrokinetic flows in such a device using said coating, as well as the micro- or nanofluidic devices comprising at least one channel or one capillary tube whereof the inner surface is covered at least partly with such a coating.

Abstract translation: 本发明涉及微流体领域，特别涉及微流体或纳流体装置上的电泳领域。 更具体地说，本发明涉及适用于在化学或物理类型的外部刺激的影响下进行相分离以调节动电流（例如电泳期间的电渗或电泳流）的反应性聚合物涂层的用途 微流体或纳流体装置。 本发明还涉及一种用于使用所述涂层改变这种装置中的电动动力流动的方法，以及包括至少一个通道或一个毛细管的微流体或纳流体装置，其中内表面至少部分地被这样的涂层 。

公开(公告)号：WO0168913A3

公开(公告)日：2002-10-31

申请号：PCT/IB0100465

申请日：2001-03-13

Applicant: GENSET SA ,  PEPONNET CHRISTINE ,  SUDOR JAN

Inventor： PEPONNET CHRISTINE ,  SUDOR JAN

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6869 ,  C12Q2600/156

Abstract: The present invention provides novel nucleic acid detection methods based on nucleic acid template-directed primer extension assays. Also provided are kits and genetic analysis methods. Included are microsequencing methods comprising detecting an incorporated nucleotide by measuring direct fluorescence intensity, fluorescence-based nucleic acid detection methods using oligonucleotide primers hybridizing to both complementary strands of a nucleic acid sample, and methods for decreasing background signal in nucleic acid template-directed primer extension assays.

Abstract translation: 本发明提供了基于核酸模板定向引物延伸测定的新型核酸检测方法。 还提供了试剂盒和遗传分析方法。 包括微测序方法，其包括通过测量直接荧光强度检测并入的核苷酸，使用与核酸样品的两条互补链杂交的寡核苷酸引物的基于荧光的核酸检测方法，以及用于降低核酸模板定向引物延伸中的背景信号的方法 试验。

公开(公告)号：WO0230571A3

公开(公告)日：2003-09-18

申请号：PCT/US0142631

申请日：2001-10-10

Applicant: GENSET SA ,  SUDOR JAN

Inventor： SUDOR JAN

IPC: G01N31/20 ,  C12Q1/68 ,  G01N33/543 ,  G01N37/00 ,  C08J7/04

CPC classification number: G01N33/54393

Abstract: The invention provides methods for the treatment of surfaces using surface adsorbing polymers, methods for decreasing the adsorption of organic materials onto the surface of treated devices or vessels, methods for performing fluid operations involving the treatment of surfaces, and apparatus and systems comprising the treated surfaces. Further, the present invention provides a method for treating the surface of microfluidics channel wherein the microfluidics surface is coated for deactivation and wherein this coating can be easily regenerated. The present invention also provides a method for treating the surface of a plastic device. The surface adsorbing polymers of the invention are particularly stable at temperatures and conditions required for biochemical reactions, especially in applications involving temperature cycling or polymerization of polynucleotides or polypeptides.

Abstract translation: 本发明提供了使用表面吸附聚合物处理表面的方法，减少有机材料吸附到经处理的装置或容器的表面上的方法，用于执行涉及表面处理的流体操作的方法以及包括处理表面的装置和系统 。 此外，本发明提供了一种处理微流体通道表面的方法，其中微流体表面被涂覆以失活，并且其中该涂层可以容易地再生。 本发明还提供了一种处理塑料装置表面的方法。 本发明的表面吸附聚合物在生化反应所需的温度和条件下特别稳定，特别是在涉及温度循环或聚合多核苷酸或多肽的应用中。