公开(公告)号：WO1996021399A1

公开(公告)日：1996-07-18

申请号：PCT/US1996000066

申请日：1996-01-11

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  COULTER, Wallace, H. ,  SHAMBAUGH, Charles, R. ,  CARTER, James, H. ,  SHUKLA, Ravindra

IPC: A61B19/00

CPC classification number: G01N35/1079 ,  A61B5/15003 ,  A61B5/150351 ,  A61B5/150755 ,  A61J1/201 ,  A61J1/2017 ,  A61J1/2058 ,  A61J1/2072 ,  A61J1/2075 ,  A61J1/2082 ,  A61J1/2086 ,  A61J1/2089 ,  A61J1/2096 ,  B01L3/5082

Abstract: A device and method for transferring fluids between closed vials is provided. In one embodiment, a reagent transfer vial (42) is provided. In a further embodiment, the reagent transfer vial is used in conjunction with a transfer device (10) which includes a cylinder having a pair of opposing open tubes or tubular portions into which a sample vial (12) and the reagent transfer vial (42) can be inserted to transfer fluid therebetween. The tubes are separated by a wall through which extends a fluid transfer needle (40) and a vent needle (24) which first is inserted into a sample vial to vent the sample vial into a vent chamber (36) or reservoir formed in the transfer device before the transfer needle is inserted into the sample vial.

Abstract translation: 提供了用于在密封小瓶之间传送流体的装置和方法。 在一个实施方案中，提供了试剂转移小瓶（42）。 在另一个实施方案中，试剂转移小瓶与转运装置（10）结合使用，转运装置（10）包括具有一对相对的开放管或管状部分的圆筒，样品瓶（12）和试剂转移小瓶（42） 可以插入其间传送流体。 管被壁分开，通过该壁延伸流体输送针（40）和通气针（24），通气针（24）首先插入样品瓶中，以将样品瓶排出到形成在转印器中的通风室（36）或储存器 将转移针插入样品瓶中之前的装置。

公开(公告)号：WO1997018474A1

公开(公告)日：1997-05-22

申请号：PCT/US1996017577

申请日：1996-11-05

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  COULTER, Wallace, H. ,  ZWERNER, Robert, K. ,  SCHMITTLING, Robert, J. ,  RUSSELL, Thomas, R.

IPC: G01N33/569

CPC classification number: G01N33/5375 ,  G01N33/569 ,  G01N33/56966

Abstract: A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles (14) have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample (12) by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernantant can be further enriched by multiple removal steps.

Abstract translation: 用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。 颗粒（14）具有一个或多个与其结合的反应物，其特定于并将与所选群体结合。 颗粒优选与样品（12）混合，通过反复使颗粒通过大部分样品沉降以结合所选择的群体。 然后允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含富集群体的上清液从结合有所选群体的颗粒中分离。 通过多次去除步骤可以进一步丰富生物样品上清液中富集的种群。