公开(公告)号：WO2021092356A1

公开(公告)日：2021-05-14

申请号：PCT/US2020/059379

申请日：2020-11-06

Applicant: DANISCO US INC

Inventor： BODIE, Elizabeth A. ,  CHEN, Zhongqiang ,  LIU, Chuanbin ,  WARD, Michael

IPC: C12N15/90

Abstract: The present disclosure is generally related to modified filamentous fungal strains (cells) comprising enhanced protein productivity phenotypes, wherein such modified strains are particularly well-suited for growth in submerged cultures (e.g., large-scale production of proteins for industrial/commercial applications). Thus, certain embodiments of the disclosure are related to such variant (modified) strains of filamentous fungus derived (obtained) from parental strains comprising a gene encoding a native GEF1 protein, wherein the variant strain comprises a genetic modification which disrupts or deletes a gene encoding a native GEF1 protein, wherein the variant strain comprises an increased protein productivity phenotype (i.e., relative to the parental strain) when grown/cultivated/fermented under the same conditions.

公开(公告)号：WO2020069063A1

公开(公告)日：2020-04-02

申请号：PCT/US2019/053061

申请日：2019-09-26

Applicant: DANISCO US INC

Inventor： CHEN, Zhongqiang ,  QI, Min ,  TAO, Luan ,  ZHU, Quinn Qun

IPC: C07K14/395 ,  C12N9/00 ,  C12P7/06

Abstract: Described are compositions and methods relating to modified yeast that over-express the glycerol-deficient suppressor, GDS1. The yeast produces an increased amount of ethanol and a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.

公开(公告)号：WO2019083879A1

公开(公告)日：2019-05-02

申请号：PCT/US2018/056861

申请日：2018-10-22

Applicant: DANISCO US INC

Inventor： FAN, Xiaochun ,  CHEN, Zhongqiang ,  QI, Min

IPC: C07K14/395 ,  C12P7/06

Abstract: Described are compositions and methods relating to yeast cells having a genetic mutation that give rise to increased alcohol production. Such yeast is well-suited for use in alcohol production to reduce fermentation time and/or increase yields.

公开(公告)号：WO2023076323A1

公开(公告)日：2023-05-04

申请号：PCT/US2022/047796

申请日：2022-10-26

Applicant: DANISCO US INC.

Inventor： BEMENT, Courtney ,  CHEN, Zhongqiang ,  FAN, Xiaochun ,  MACOOL, Daniel Joseph ,  QI, Min ,  WANG, Yehong Jamie ,  XUE, Zhixiong ,  ZHU, Quinn Qun

IPC: C12N9/22 ,  C07K14/395 ,  C12N15/81 ,  C12P7/04 ,  C12P7/06

Abstract: Described are compositions and methods relating to modified yeast with disrupted RSF2 or TDA9 genes. The yeast produces a decreased amount of acetate compared to otherwise identical parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate is an undesirable end product.

公开(公告)号：WO2021150911A1

公开(公告)日：2021-07-29

申请号：PCT/US2021/014641

申请日：2021-01-22

Applicant: DANISCO US INC

Inventor： CHEN, Zhongqiang ,  WANG, Yehong Jamie ,  ZHU, Quinn Qun

IPC: C12N1/18 ,  C12N15/63 ,  C12P7/02 ,  C12P7/06 ,  C07K14/395 ,  C12N15/81 ,  C12R2001/865

Abstract: Described are compositions and methods relating to modified yeast that overexpresses the J-protein, JID1. The yeast produces an increased amount of ethanol compared to otherwise identical parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates.

公开(公告)号：WO2020186254A1

公开(公告)日：2020-09-17

申请号：PCT/US2020/022853

申请日：2020-03-14

Applicant: DANISCO US INC

Inventor： KRASLEY, Elizabeth A. ,  CHEN, Zhongqiang ,  MAGGIO-HALL, Lori Ann ,  ZHU, Quinn Qun

IPC: C12N15/81 ,  C12P7/06 ,  C07K14/395

Abstract: Described are compositions and methods relating to modified yeast that over-express fumarate-succinate transporter. The yeast produces an increased amount of ethanol and decreased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate is an undesired by-product.