-
公开(公告)号：WO2010031772A3
公开(公告)日：2010-06-10
申请号：PCT/EP2009061961
申请日：2009-09-15
申请人： DSM IP ASSETS BV , WU LIANG , PERKINS JOHN B , SCHYNS GHISLAIN
发明人： WU LIANG , PERKINS JOHN B , SCHYNS GHISLAIN
CPC分类号： C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1022 , C12N9/1029 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.
摘要翻译： 本发明涉及一种微生物细胞，其包含编码酶的基因，其催化以下反应：a）丙酮酸至乙酰乳酸，b）乙酰乳酸至2,3-二羟基异戊酸酯，c）2,3-二羟基异戊酸酯为2-酮异戊酸酯，d）2-酮异戊酸 异丁酰辅酶A，异丁酰辅酶A，丁酰辅酶A，f）丁酰辅酶A，丁醛，和g）丁醛至丁醇，其中该细胞产生丁醇。 本发明还涉及生产丁醇的方法，其中使用本发明的微生物细胞。
-
公开(公告)号：WO2009013159A2
公开(公告)日：2009-01-29
申请号：PCT/EP2008059119
申请日：2008-07-11
申请人： DSM IP ASSETS BV , MUELLER ULRIKE MARIA , WU LIANG , RAAMSDONK LOURINA MADELEINE , WINKLER AARON ADRIAAN
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
摘要翻译： 本发明涉及鉴定具有酶促活性的异源多肽的方法，所述酶促活性用于将丙酮酸，乙醛或乙酸转化为酵母细胞（的胞质溶胶）中的乙酰-CoA，所述方法包括：a）提供突变的酵母细胞，所述突变的酵母细胞包含至少 选自编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和乙酰-CoA合成酶（ACS）的基因的（PDH）旁路的一个基因; b）用表达载体转化所述突变的酵母细胞，所述表达载体包含编码具有用于将丙酮酸，乙醛或乙酸转化成乙酰-CoA的潜在酶活性的候选多肽的异源核苷酸序列; c）测试所述重组突变的酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力，和d）将所述候选多肽鉴定为具有用于将丙酮酸，乙醛或乙酸转化为乙酰辅酶A的酶活性的异源多肽 当观察到所述细胞的生长时，所述酵母细胞的胞质溶胶）。 本发明进一步涉及生产发酵生产如丁醇的方法。
-
公开(公告)号：WO2009101180A3
公开(公告)日：2009-11-26
申请号：PCT/EP2009051717
申请日：2009-02-13
申请人： DSM IP ASSETS BV , VERWAAL RENE , WU LIANG , DAMVELD ROBBERTUS ANTONIUS , SAGT CORNELIS MARIA JACOBUS
IPC分类号： C12P7/46
CPC分类号： C12P7/46
摘要： The present invention relates to a process for the production of a dicarboxylic acid wherein a eukaryotic cell is fermented in a suitable fermentation medium. The invention further relates to a eukaryotic cell comprising a nucleotide sequence encoding an enzyme which catalyses the conversion of isocitric acid to succinic acid, and a nucleotide sequence encoding an enzyme which catalyses the conversion of glyoxylic acid to malic acid.
摘要翻译： 本发明涉及一种生产二羧酸的方法，其中真核细胞在合适的发酵培养基中发酵。 本发明进一步涉及包含编码催化异柠檬酸向琥珀酸的转化的酶的核苷酸序列的真核细胞和编码催化乙醛酸向苹果酸转化的酶的核苷酸序列。
-
公开(公告)号：WO2009113853A2
公开(公告)日：2009-09-17
申请号：PCT/NL2009050115
申请日：2009-03-11
申请人： DSM IP ASSETS BV , WU LIANG , TREFZER AXEL CHRISTOPH , DE WILDEMAN STEFAAN MARIE ANDR , VAN DEN BERG MARCO ALEXANDER
发明人： WU LIANG , TREFZER AXEL CHRISTOPH , DE WILDEMAN STEFAAN MARIE ANDRE , VAN DEN BERG MARCO ALEXANDER
IPC分类号： C12P7/44
摘要： The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid(6-ACA), a method for preparing 5-formyl valeric acid (5- FVA), and a method for preparingcaprolactam. Further, the invention relates to a host cell for use in a method according to the invention.
摘要翻译： 本发明涉及一种制备己二酸酯或硫酯的方法。 本发明还涉及从所述酯或硫酯制备己二酸的方法。 此外，本发明提供了许多用于制备所述酯或硫酯的中间体的方法。 本发明还涉及一种制备6-氨基己酸（6-ACA）的方法，5-甲酰戊酸（5-FVA）的制备方法及其制备己内酰胺的方法。 此外，本发明涉及用于根据本发明的方法的宿主细胞。
-
公开(公告)号：WO2015181312A3
公开(公告)日：2016-01-21
申请号：PCT/EP2015061884
申请日：2015-05-28
申请人： DSM IP ASSETS BV
发明人： ZHAO ZHENG , MEIJRINK BERNARD , HOEVEN VAN DER ROBERTUS ANTONIUS MIJNDERT , WU LIANG , ROUBOS JOHANNES ANDRIES , GELDER VAN MARINA
摘要： The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid,4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which over expresses: -a nucleic 1 acid encoding a polypeptide having cis-aconitate decarboxylase activity; and-one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA,wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans- aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
摘要翻译： 本发明涉及能够产生衣康酸，衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组细胞，其中所述重组微生物已经在其基因组中被修饰，从而导致其中的缺陷 产生反式乌头碱甲基转移酶。 能够产生衣康酸的重组酵母细胞，其过表达： - 编码具有顺式乌头酸脱羧酶活性的多肽的核酸1; 一种或多种编码分别或一起催化乙酰CoA的反应的多肽的核酸，其中所述重组微生物已经在其基因组中被修饰，从而导致转基因甲基转移酶的产生缺陷。 这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。
-
公开(公告)号：WO2014202616A3
公开(公告)日：2015-04-09
申请号：PCT/EP2014062737
申请日：2014-06-17
申请人： DSM IP ASSETS BV
发明人： WU LIANG
CPC分类号： C07K14/37
摘要： The invention relates to apolypeptide which comprises (a) an amino acid sequence set out in SEQ ID NO: 20501 to 32240; (b) an amino acid sequence encoded by the nucleic acid sequence of SEQ ID NO: 1 to 20500; (c) or a variant polypeptide, wherein the variant polypeptide (i) has at least 70% sequence identity with the sequence set out in SEQ ID NO: 20501 to 32240, or (ii) has an amino acid sequence that differs in, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 amino acids from the amino acid sequence of SEQ ID NO: 20501 to 32240. The invention also relates to methods for using the polypeptide in industrial processes. 1 Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
摘要翻译： 本发明涉及多肽，其包含（a）SEQ ID NO：20501至32240所示的氨基酸序列; （b）由SEQ ID NO：1至20500的核酸序列编码的氨基酸序列; （c）或变体多肽，其中所述变体多肽（i）与SEQ ID NO：20501至32240所示的序列具有至少70％的序列同一性，或（ii）具有不同于2的氨基酸序列 ，3,4,5,6,7,8,9,10,11或12个氨基酸。本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。
-
7.发明申请
公开(公告)号：WO2015181310A3
公开(公告)日：2016-02-18
申请号：PCT/EP2015061882
申请日：2015-05-28
申请人： DSM IP ASSETS BV
发明人： ZHAO ZHENG , MEIJRINK BERNARD , HOEVEN VAN DER ROBERTUS ANTONIUS MIJNDERT , WU LIANG , ROUBOS JOHANNES ANDRIES
CPC分类号： C12P7/44 , C07K14/395 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/62 , C12Y203/03001 , C12Y401/01006 , C12Y401/01031 , C12Y401/01032 , C12Y604/01001
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: - a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and - a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
摘要翻译： 本发明涉及能够产生衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯中的一种或多种的重组酵母细胞。 本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸; 以及 - 编码催化向乙酰辅酶A反应的多肽的核酸。 这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯的方法。
-
8.发明申请
公开(公告)号：WO2014080024A3
公开(公告)日：2014-09-18
申请号：PCT/EP2013074658
申请日：2013-11-25
申请人： DSM IP ASSETS BV
发明人： ZHAO ZHENG , MEIJRINK BERNARD , HOEVEN VAN DER ROBERTUS ANTONIUS MIJNDERT , WU LIANG , ROUBOS JOHANNES ANDRIES
CPC分类号： C12P7/46 , C12N9/88 , C12N15/81 , C12P7/62 , C12Y401/01006
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
摘要翻译： 本发明涉及能够产生衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组酵母细胞。 本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞：编码具有顺式乌头酸脱羧酶活性的多肽的核酸; 以及编码催化向乙酰辅酶A反应的多肽的核酸。 这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.061 秒)
