公开(公告)号：WO2016209938A1

公开(公告)日：2016-12-29

申请号：PCT/US2016/038731

申请日：2016-06-22

Applicant: ENVIROLOGIX INC.

Inventor： SHAFFER, Daniel ,  JUDICE, Stephen A. ,  HARDINGHAM, Jonathan

IPC: C12N15/10 ,  G01N1/34 ,  G01N33/50

CPC classification number: C12Q1/6806 ,  B01L9/54 ,  G01N1/40 ,  G01N2001/4088 ,  C12Q2523/303

Abstract: The present invention features compositions and methods to purify biological samples (e.g., human blood derived leukocytes) using electricity-free tools without cross-contamination to obtain an analyte (e.g., RNA) for downstream diagnostic applications.

Abstract translation: 本发明的特征在于使用无交叉污染的无电极工具来净化生物样品（例如，人血源性白细胞）的组合物和方法，以获得用于下游诊断应用的分析物（例如RNA）。

公开(公告)号：WO2016064894A2

公开(公告)日：2016-04-28

申请号：PCT/US2015/056491

申请日：2015-10-20

Applicant: ENVIROLOGIX INC.

Inventor： PETERS, Lars ,  JUDICE, Stephen, A. ,  SHAFFER, Daniel ,  PARKER, Breck

IPC: C12Q1/68

CPC classification number: C12Q1/701 ,  C12Q1/6865 ,  C12Q2600/158 ,  Y02A50/53 ,  Y02A50/60 ,  C12Q2521/107

Abstract: The present invention provides methods for rapidly identifying an RNA viral infection using an isothermal nucleic acid amplification reaction that can be carried out extracted RNA in the context of a crude biological sample.

Abstract translation: 本发明提供了使用等温核酸扩增反应来快速鉴定RNA病毒感染的方法，所述等温核酸扩增反应可以在粗生物样品的情况下进行提取的RNA。

公开(公告)号：WO2015164494A1

公开(公告)日：2015-10-29

申请号：PCT/US2015/027074

申请日：2015-04-22

Applicant: ENVIROLOGIX, INC.

Inventor： PETERS, Lars ,  JUDICE, Stephen A. ,  SHAFFER, Daniel ,  PARKER, Breck

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6895 ,  C07H21/04 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2600/156 ,  C12Q2531/119

Abstract: The present invention features compositions and methods for amplifying a target oligonucleotide in a sample, including detection of the target oligonucleotide in real time.

Abstract translation: 本发明的特征在于用于扩增样品中靶寡核苷酸的组合物和方法，包括实时检测靶寡核苷酸。

公开(公告)号：WO2016069345A1

公开(公告)日：2016-05-06

申请号：PCT/US2015/056641

申请日：2015-10-21

Applicant: ENVIROLOGIX INC.

Inventor： SHAFFER, Daniel

IPC: C12N1/08 ,  C12M3/00 ,  B01D63/00 ,  B01D27/00 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/6837 ,  G01N33/68 ,  C12Q2565/629

Abstract: The invention generally provides a sample preparation vessel including a flexible substrate defining at least one sealable opening adapted and configured to receive a solid sample; at least one fitting; and at least one filter adjacent to the at least one fitting, the filter adapted and configured to permit extracted fluids to exit the vessel while retaining solid particles, as well as vessels, circuits, systems, and related methods for sample preparation, extraction, and analysis.

Abstract translation: 本发明通常提供一种样品制备容器，其包括限定至少一个可密封的开口的柔性基底，所述可密封开口适于并构造成容纳固体样品; 至少一个配件; 以及与所述至少一个配件相邻的至少一个过滤器，所述过滤器适于并且构造成允许提取的流体离开所述容器同时保留固体颗粒，以及容器，回路，系统和用于样品制备，提取和 分析。

公开(公告)号：WO2013155056A9

公开(公告)日：2013-10-17

申请号：PCT/US2013/035750

申请日：2013-04-09

Applicant: ENVIROLOGIX INC. ,  SHAFFER, Daniel ,  JUDICE, Stephen, A.

Inventor： SHAFFER, Daniel ,  JUDICE, Stephen, A.

IPC: C12Q1/68 ,  C12Q1/00 ,  C12N15/11

Abstract: The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.