公开(公告)号：WO2013182206A1

公开(公告)日：2013-12-12

申请号：PCT/DK2013/050182

申请日：2013-06-07

Applicant: GLYCOM A/S

Inventor： PELTIER-PAIN, Pauline ,  DEKANY, Gyula ,  DUREAU, Remy ,  RISINGER, Christian ,  HEDEROS, Markus ,  CHAMPION, Elise

IPC: C12P19/44 ,  C07H3/06 ,  C07H5/04 ,  C07H5/08 ,  C07H15/18 ,  C12P19/28 ,  C12P19/64

CPC classification number: C07H3/06 ,  C07H5/04 ,  C07H15/10 ,  C07H15/14 ,  C07H15/18 ,  C12P19/28 ,  C12P19/44 ,  C12P19/64 ,  Y02P20/55

Abstract: The application discloses a method for producing anomerically protected glycosidic oligosaccharide derivatives comprising the step of culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor. The application further discloses a method for producing an oligosaccharide comprising the steps of: (a) culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor to produce an anomerically protected glycosidic oligosaccharide derivative, then (b) removing/deprotecting the anomeric protective group.

Abstract translation: 本申请公开了一种用于制备异头保护的糖苷寡糖衍生物的方法，其包括在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞的步骤 活化的糖核苷酸与所述乳糖受体。 本申请进一步公开了一种生产寡糖的方法，包括以下步骤：（a）在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞 的活性糖核苷酸与所述乳糖受体产生异头保护的糖苷寡糖衍生物，然后（b）去除/去保护端基异构保护基。