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    OLIGOSACCHARIDE PRODUCTION
    1.
    发明申请
    OLIGOSACCHARIDE PRODUCTION 审中-公开
    低聚糖生产

    公开(公告)号：WO2015197082A1

    公开(公告)日：2015-12-30

    申请号：PCT/DK2015/050191

    申请日：2015-06-29

    Applicant: GLYCOM A/S

    Inventor： SAMAIN, Eric ,  PELTIER-PAIN, Pauline ,  BYCH, Katrine ,  JOHANSON, Ted ,  CHAMPION, Elise ,  DEKANY, Gyula

    IPC: C12P19/18 ,  C12N1/21

    CPC classification number: C12P19/18 ,  C07K14/245 ,  C12N9/1029 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/52 ,  C12Y203/01018 ,  C12Y204/01149 ,  C12Y302/01023 ,  C12Y302/01026

    Abstract: The invention relates to a genetically modified microorganism for making a recombinant oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source. The one or more genes encoding a sucrose utilization system are preferably one or more genes encoding a heterologous PTS-dependent sucrose utilization transport system, such as the scr genes.

    Abstract translation: 本发明涉及用于制备重组寡糖的转基因微生物，优选3-8个单糖单元，更优选3-5个单糖单元，特别是包含编码蔗糖利用系统的一个或多个基因的HMO，因此微生物 可以使用蔗糖作为碳源和能源。 编码蔗糖利用系统的一种或多种基因优选是编码异源PTS依赖性蔗糖利用转运系统（例如scr基因）的一种或多种基因。

    METHOD FOR PRODUCING OLIGOSACCHARIDES AND OLIGOSACCHARIDE GLYCOSIDES BY FERMENTATION
    2.
    发明申请
    METHOD FOR PRODUCING OLIGOSACCHARIDES AND OLIGOSACCHARIDE GLYCOSIDES BY FERMENTATION 审中-公开
    通过发酵生产低聚葡萄糖苷和低聚果糖苷的方法

    公开(公告)号：WO2013182206A1

    公开(公告)日：2013-12-12

    申请号：PCT/DK2013/050182

    申请日：2013-06-07

    Applicant: GLYCOM A/S

    Inventor： PELTIER-PAIN, Pauline ,  DEKANY, Gyula ,  DUREAU, Remy ,  RISINGER, Christian ,  HEDEROS, Markus ,  CHAMPION, Elise

    IPC: C12P19/44 ,  C07H3/06 ,  C07H5/04 ,  C07H5/08 ,  C07H15/18 ,  C12P19/28 ,  C12P19/64

    CPC classification number: C07H3/06 ,  C07H5/04 ,  C07H15/10 ,  C07H15/14 ,  C07H15/18 ,  C12P19/28 ,  C12P19/44 ,  C12P19/64 ,  Y02P20/55

    Abstract: The application discloses a method for producing anomerically protected glycosidic oligosaccharide derivatives comprising the step of culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor. The application further discloses a method for producing an oligosaccharide comprising the steps of: (a) culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor to produce an anomerically protected glycosidic oligosaccharide derivative, then (b) removing/deprotecting the anomeric protective group.

    Abstract translation: 本申请公开了一种用于制备异头保护的糖苷寡糖衍生物的方法，其包括在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞的步骤 活化的糖核苷酸与所述乳糖受体。 本申请进一步公开了一种生产寡糖的方法，包括以下步骤：（a）在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞 的活性糖核苷酸与所述乳糖受体产生异头保护的糖苷寡糖衍生物，然后（b）去除/去保护端基异构保护基。

    SYNTHESIS OF SIALYLATED/FUCOSYLATED HUMAN MILK OLIGOSACCHARIDES
    4.
    发明申请
    SYNTHESIS OF SIALYLATED/FUCOSYLATED HUMAN MILK OLIGOSACCHARIDES 审中-公开
    异亮氨酸/ FOSOSLIL人乳酪寡糖的合成

    公开(公告)号：WO2014167538A1

    公开(公告)日：2014-10-16

    申请号：PCT/IB2014/060648

    申请日：2014-04-11

    Applicant: GLYCOM A/S

    Inventor： CHASSAGNE, Pierre ,  KHANZHIN, Nikolay ,  HEDEROS, Markus ,  CHAMPION, Elise ,  MATWIEJUK, Martin ,  DEKANY, Gyula

    IPC: C07H1/00 ,  C07H5/04

    CPC classification number: C07H5/04 ,  C07H1/00

    Abstract: This invention relates to achemo-enzymatic synthesis of oligosaccharides of formula 1 I wherein R is selected from -OH, -N 3 and -OR 6 wherein R 6 is selected from allyl optionally substituted by one or more methyl, propargyl optionally substituted by one or more methyl, 2-trimethylsilyl-ethyl, -(CH 2 ) n -NH 2 and -(CH 2 ) n -N 3 wherein integer n is to 10,preferably 2 or 3, R is selected from sialyl moiety, -SO 3 H and -CH(R )-COOH wherein R is selected from H, alkyl and benzyl, R 2 is selected from H and fucosyl, R 3 is selected from H and sialyl, R 4 is selected from H and fucosyl, provided that at leastone of R 3 and R 4 is H, and A is a divalent carbohydrate linker, having important biological activities and significant commercial value for the pharmaceutical and food industry.

    Abstract translation: 本发明涉及式Ⅰ的寡糖的急速 - 酶合成，其中R选自-OH，-N 3和-OR 6，其中R 6选自任选被一个或多个甲基取代的烯丙基，炔丙基任选地被一个或多个 更多的甲基，2-三甲基甲硅烷基 - 乙基， - （CH 2）n -NH 2和 - （CH 2）n -N 3其中整数n为10，优选2或3，R选自唾液酰基部分，-SO 3 H和-CH（R）-COOH其中R选自H，烷基和苄基，R 2选自H和岩藻糖基，R 3选自H和唾液酸，R 4选自H和岩藻糖基，条件是在 R 3和R 4的leastone为H，且A为二价连接基的碳水化合物，其具有重要的生物活性和用于医药和食品工业显著商业价值。

    DIVERSIFICATION OF HUMAN MILK OLIGOSACCHARIDES (HMOs) OR PRECURSORS THEREOF
    5.
    发明申请
    DIVERSIFICATION OF HUMAN MILK OLIGOSACCHARIDES (HMOs) OR PRECURSORS THEREOF 审中-公开
    人乳清蛋白（HMOs）或其前体的多样性

    公开(公告)号：WO2012156898A1

    公开(公告)日：2012-11-22

    申请号：PCT/IB2012/052401

    申请日：2012-05-14

    Applicant: GLYCOM A/S ,  DEKANY, Gyula ,  CHAMPION, Elise ,  SCHROVEN, Andreas ,  HEDEROS, Markus

    Inventor： DEKANY, Gyula ,  CHAMPION, Elise ,  SCHROVEN, Andreas ,  HEDEROS, Markus

    IPC: C07H5/04 ,  C07H1/00 ,  C07H3/06

    CPC classification number: C12P19/14 ,  C07H1/00 ,  C07H5/04 ,  C07H13/04 ,  C12P19/04 ,  C12P19/12

    Abstract: A method of diversification of human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprises the steps of a) providing at least one compound or a mixture of the compounds selected from the group consisting of: optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof: (2) wherein R is a group removable by hydrogenolysis, R 1 independently of each other is fucosyl or H, R 4 independently of each other is sialyl or H, provided that the compound of general formula 2 is not R-glycoside of lactose, if provided alone; optionally sialylated and/or fucosylated lactose derivatives of general formula 4 and salts thereof: (4) wherein R 1 independently of each other is fucosyl or H, R 4 independently of each other is sialyl or H, provided that the compound of general formula 4 is not lactose, if provided alone; lacto-N-tetraose (LNT): (I) lacto-N-tetraose (LNT) derivatives of the following formula: (II) wherein R is a group removable by hydrogenolysis; lacto-N-neotetraose (LNnT): (III) lacto-N-neotetraose (LNnT) derivatives of the following formula: (IV) wherein R is a group removable by hydrogenolysis; b) adding at least one enzyme comprising a transglycosidase activity to the at least one compound or a mixture of compounds provided according to step a); and c) incubating the mixture obtained according to step b).

    Abstract translation: 人乳寡糖（HMO）或其前体多样化的方法，可通过该方法获得的化合物以及涉及这些化合物的用途和组合物。 该方法包括以下步骤：a）提供至少一种化合物或选自以下的化合物或混合物：选自唾液酸化的和/或通式2的岩藻糖基化的乳糖衍生物及其盐：（2）其中R是基团 通过氢解除去，R 1彼此独立地是岩藻糖基或H，R 4彼此独立地是唾液酸或H，条件是通式2的化合物不是单独提供的乳糖的R-糖苷; 任选的通式4的唾液酸化和/或岩藻糖化的乳糖衍生物及其盐：（4）其中R 1彼此独立地是岩藻糖基或H，R 4彼此独立地是唾液酸或H，条件是通式4的化合物不是 乳糖，如果单独提供; 乳糖-N-四糖（LNT）：（I）乳-N-差氮（LNT）衍生物：（II）其中R是可通过氢解除去的基团; 乳酸-N-新四糖（LNnT）：（III）下式的乳-N-新四糖（LNnT）衍生物：（Ⅳ）其中R是通过氢解除去的基团; b）向所述至少一种化合物或根据步骤a）提供的化合物的混合物中加入至少一种包含转糖苷酶活性的酶; 和c）孵育根据步骤b）获得的混合物。

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