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公开(公告)号:WO2017143301A1
公开(公告)日:2017-08-24
申请号:PCT/US2017/018523
申请日:2017-02-17
Applicant: HAMPTON CREEK, INC.
Inventor: BANSAL-MUTALIK, Ritu , BHIDE, Siddharth , GIBSON, Brenna , HALL, Camilla , JAKUBASCH, Malgorzata , KLEINER, Jake , LANQUAR, Viviane , MAHADEVAN, Swetha , NIEKOWAL, Trevor , PROULX, Jade , ROCHE, Ben , XU, Meng , FLATT, James
IPC: A23J1/14
CPC classification number: A23L11/09 , A21D10/002 , A21D13/40 , A23C11/06 , A23C19/076 , A23G9/38 , A23J1/14 , A23J3/227 , A23L2/52 , A23L2/66 , A23L7/109 , A23L13/426 , A23L15/35 , A23L27/80 , A23L33/105 , A23V2002/00 , A23V2200/122 , A23V2250/548 , A23V2300/14 , A23V2300/18 , A23V2300/40
Abstract: Provided herein are methods for producing an adzuki bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more adzuki bean proteins from an adzuki bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.
Abstract translation: 本文提供了用于生产具有高功能性的小豆蛋白质分离物用于广泛的食品应用的方法。 在一些实施方案中,用于产生分离物的方法包括选自以下的一个或多个步骤:(a)在水溶液中从小红豆蛋白源提取一种或多种小红豆蛋白质,例如在约6.5-10.0 ; (b)使用两种方法中的至少一种从提取物中纯化蛋白质:(i)在接近富含球蛋白部分的等电点的pH下,例如约5.0-6.0的pH下,从提取物中沉淀蛋白质; 和/或(ii)使用过滤如微滤,超滤或离子交换层析从提取物中分离和浓缩蛋白质; 和(c)回收纯化的蛋白质分离物。 p>
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公开(公告)号:WO2017143298A1
公开(公告)日:2017-08-24
申请号:PCT/US2017/018519
申请日:2017-02-17
Applicant: HAMPTON CREEK, INC.
Inventor: BANSAL-MUTALIK, Ritu , BHIDE, Siddharth , GIBSON, Brenna , HALL, Camilla , JAKUBASCH, Malgorzata , KLEINER, Jake , LANQUAR, Viviane , MAHADEVAN, Swetha , NIEKOWAL, Trevor , PROULX, Jade , ROCHE, Ben , XU, Meng , FLATT, James , PARK, Nathaniel
IPC: A23J1/14
CPC classification number: A23L15/35 , A21D13/40 , A23C9/1526 , A23C13/12 , A23C15/12 , A23G9/38 , A23J1/14 , A23J3/227 , A23L2/66 , A23L7/109 , A23L13/426 , A23V2002/00 , C07K1/36 , C07K14/415 , A23V2200/122 , A23V2250/548 , A23V2300/14 , A23V2300/18 , A23V2300/40
Abstract: Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.
Abstract translation: 本文提供了用于生产具有高功能性的绿豆蛋白质分离物用于广泛的食品应用的方法。 在一些实施方案中,用于产生分离物的方法包括选自以下的一个或多个步骤:(a)在水溶液中从绿豆蛋白质源提取一种或多种绿豆蛋白质,例如在约6.5-10.0 ; (b)使用两种方法中的至少一种从提取物中纯化蛋白质:(i)在接近富含球蛋白部分的等电点的pH下,例如约5.0-6.0的pH下,从提取物中沉淀蛋白质; 和/或(ii)使用过滤如微滤,超滤或离子交换层析从提取物中分离和浓缩蛋白质; 和(c)回收纯化的蛋白质分离物。 p>
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