公开(公告)号：WO2017059250A1

公开(公告)日：2017-04-06

申请号：PCT/US2016/054777

申请日：2016-09-30

Applicant: HAMPTON CREEK, INC. ,  CHAE, Lee ,  TETRICK, Josh, Stephen ,  XU, Meng ,  SCHULTZ, Matthew, D. ,  WANG, Chuan ,  TILMANS, Nicolas ,  BRZUSTOWICZ, Michael

Inventor： CHAE, Lee ,  TETRICK, Josh, Stephen ,  XU, Meng ,  SCHULTZ, Matthew, D. ,  WANG, Chuan ,  TILMANS, Nicolas ,  BRZUSTOWICZ, Michael

IPC: G06N3/02

CPC classification number: G06N5/048 ,  G06F19/10 ,  G06N99/005

Abstract: Systems and methods for assaying a test entity for a property, without measuring the property, are provided. Exemplary test entities include proteins, protein mixtures, and protein fragments. Measurements of first features in a respective subset of an N-dimensional space and of second features in a respective subset of an M-dimensional space, is obtained as training data for each reference in a plurality of reference entities. One or more of the second features is a metric for the target property. A subset of first features, or combinations thereof, is identified using feature selection. A model is trained on the subset of first features using the training data. Measurement values for the subset of first features for the test entity are applied to thereby obtaining a model value that is compared to model values obtained using measured values of the subset of first features from reference entities exhibiting the property.

Abstract translation: 提供了用于测定属性的测试实体而不测量属性的系统和方法。 示例性测试实体包括蛋白质，蛋白质混合物和蛋白质片段。 作为用于多个参考实体中的每个参考的训练数据，获得N维空间的相应子集中的第一特征和M维空间的相应子集中的第二特征的度量。 第二个特征中的一个或多个是目标属性的度量。 使用特征选择来识别第一特征或其组合的子集。 使用训练数据对第一特征子集进行训练。 应用用于测试实体的第一特征的子集的测量值，从而获得与使用展示该属性的参考实体的第一特征子集的测量值获得的模型值进行比较的模型值。

公开(公告)号：WO2017143301A1

公开(公告)日：2017-08-24

申请号：PCT/US2017/018523

申请日：2017-02-17

Applicant: HAMPTON CREEK, INC.

Inventor： BANSAL-MUTALIK, Ritu ,  BHIDE, Siddharth ,  GIBSON, Brenna ,  HALL, Camilla ,  JAKUBASCH, Malgorzata ,  KLEINER, Jake ,  LANQUAR, Viviane ,  MAHADEVAN, Swetha ,  NIEKOWAL, Trevor ,  PROULX, Jade ,  ROCHE, Ben ,  XU, Meng ,  FLATT, James

IPC: A23J1/14

CPC classification number: A23L11/09 ,  A21D10/002 ,  A21D13/40 ,  A23C11/06 ,  A23C19/076 ,  A23G9/38 ,  A23J1/14 ,  A23J3/227 ,  A23L2/52 ,  A23L2/66 ,  A23L7/109 ,  A23L13/426 ,  A23L15/35 ,  A23L27/80 ,  A23L33/105 ,  A23V2002/00 ,  A23V2200/122 ,  A23V2250/548 ,  A23V2300/14 ,  A23V2300/18 ,  A23V2300/40

Abstract: Provided herein are methods for producing an adzuki bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more adzuki bean proteins from an adzuki bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

Abstract translation: 本文提供了用于生产具有高功能性的小豆蛋白质分离物用于广泛的食品应用的方法。 在一些实施方案中，用于产生分离物的方法包括选自以下的一个或多个步骤：（a）在水溶液中从小红豆蛋白源提取一种或多种小红豆蛋白质，例如在约6.5-10.0 ; （b）使用两种方法中的至少一种从提取物中纯化蛋白质：（i）在接近富含球蛋白部分的等电点的pH下，例如约5.0-6.0的pH下，从提取物中沉淀蛋白质; 和/或（ii）使用过滤如微滤，超滤或离子交换层析从提取物中分离和浓缩蛋白质; 和（c）回收纯化的蛋白质分离物。

公开(公告)号：WO2017143298A1

公开(公告)日：2017-08-24

申请号：PCT/US2017/018519

申请日：2017-02-17

Applicant: HAMPTON CREEK, INC.

Inventor： BANSAL-MUTALIK, Ritu ,  BHIDE, Siddharth ,  GIBSON, Brenna ,  HALL, Camilla ,  JAKUBASCH, Malgorzata ,  KLEINER, Jake ,  LANQUAR, Viviane ,  MAHADEVAN, Swetha ,  NIEKOWAL, Trevor ,  PROULX, Jade ,  ROCHE, Ben ,  XU, Meng ,  FLATT, James ,  PARK, Nathaniel

IPC: A23J1/14

CPC classification number: A23L15/35 ,  A21D13/40 ,  A23C9/1526 ,  A23C13/12 ,  A23C15/12 ,  A23G9/38 ,  A23J1/14 ,  A23J3/227 ,  A23L2/66 ,  A23L7/109 ,  A23L13/426 ,  A23V2002/00 ,  C07K1/36 ,  C07K14/415 ,  A23V2200/122 ,  A23V2250/548 ,  A23V2300/14 ,  A23V2300/18 ,  A23V2300/40

Abstract: Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

Abstract translation: 本文提供了用于生产具有高功能性的绿豆蛋白质分离物用于广泛的食品应用的方法。 在一些实施方案中，用于产生分离物的方法包括选自以下的一个或多个步骤：（a）在水溶液中从绿豆蛋白质源提取一种或多种绿豆蛋白质，例如在约6.5-10.0 ; （b）使用两种方法中的至少一种从提取物中纯化蛋白质：（i）在接近富含球蛋白部分的等电点的pH下，例如约5.0-6.0的pH下，从提取物中沉淀蛋白质; 和/或（ii）使用过滤如微滤，超滤或离子交换层析从提取物中分离和浓缩蛋白质; 和（c）回收纯化的蛋白质分离物。