Abstract:
Provided is a discharge device for discharging pretreated biomass from a pressurized reactor. The device comprises: -a vessel having an opening to a high pressure region at the top, and configured to be connected with a pressurized biomass pretreatment device; -one or more inlet openings situated along the sides of the vessels through which water or liquid may be added; -an orifice or valve at a lower part of said vessel, said orifice or valve being configured to eject pretreated biomass, optionally into a pipeline. The discharge device is characterised in that it comprises mechanical agitation means, said agitation means comprising an agitation element arranged in the interior of said vessel at a lower part of said vessel, and being configured to provide agitation of the content of said vessel, wherein said agitation means being adapted to withstand a pressure in the interior of a said vessel of 10bar or more. The agitation means provides for a temperature equalization within a specific vertical range of heights of an aqueous slurry present in said vessel, thereby eliminating disadvantages of the prior art devices and methods.
Abstract:
The invention relates to a method of processing lignocellulosic biomass comprising: providing lignocellulosic biomass feedstock; pretreating said biomass feedstock by continuous hydrothermal pretreatment in a pressurized reactor; measuring xylan number or lignin number in the output stream of pretreated biomass from the pretreatment reactor; and controlling the pretreatment reactor so as to maintain, in the output stream of pretreated biomass, a pre-determined level of measured xylan number or lignin number. The method allows for continuously controlling the digestibility of a lignocellulosic biomass during the step of pretreatment, and accordingly provides a continuous estimate of the severity of the pretreatment.
Abstract:
Provided are methods of processing empty fruit bunches (EFB) using cellulase enzymatic hydrolysis in multiple stages. Hydrolysis yields are improved in a multiple-stage process featuring at least one solid/liquid separation step to an extent that is surprisingly great, much more than can be accounted for by alleviation of glucose product inhibition of cellulase enzymes.
Abstract:
The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subject to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.