公开(公告)号：WO2016190817A1

公开(公告)日：2016-12-01

申请号：PCT/SG2016/050248

申请日：2016-05-26

Applicant: JN MEDSYS PTE LTD

Inventor： NG, Kian Kok Johnson ,  TEU, Koon Kiat ,  LING, Mei Tze Belinda ,  SIM, Jia Wen

IPC: G01N1/28 ,  G01N1/40 ,  B01L3/00

CPC classification number: G01N1/40 ,  B01L3/5082 ,  B01L3/50857 ,  B01L2200/06 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/043 ,  B01L2300/0832 ,  B01L2300/0838 ,  B01L2400/0406 ,  B01L2400/049 ,  C12Q1/6848 ,  C12Q1/686

Abstract: There is provided a method of charging an array of micro-capillaries, the micro-capillaries having at least one end that is open for fluid communication, the method comprising the steps of: (a) filling the array of micro-capillaries with an assay liquid; (b) controllably evaporating at least some of the assay liquid to remove it from the microcapillary and creating a void space in each of the capillaries between the assay liquid and the open end; and (c) filling the void space with a liquid that is immiscible with said assay liquid. There is also provided a use of the disclosed method for conducting a polymerase chain reaction and a device for charging an array of micro-capillaries.

Abstract translation: 提供了一种对微毛细管阵列进行充电的方法，所述微毛细管具有至少一个开放的用于流体连通的端部，所述方法包括以下步骤：（a）用测定法填充微毛细管阵列 液体; （b）可控地蒸发至少一些分析液体以将其从微毛细管中除去并在测定液体和开放端之间的每个毛细管中产生空隙空间; 和（c）用与所述测定液体不混溶的液体填充空隙空间。 还提供了用于进行聚合酶链反应的所公开的方法的使用和用于对微毛细管阵列进行充电的装置。