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    COINCIDENCE DETECTION
    1.
    发明申请
    COINCIDENCE DETECTION 审中-公开
    联合检测

    公开(公告)号：WO2011161420A3

    公开(公告)日：2012-05-10

    申请号：PCT/GB2011000953

    申请日：2011-06-23

    申请人： KING S COLLEGE LONDON ,  PARKER PETER ,  PERANI MICHELA

    发明人： PARKER PETER ,  PERANI MICHELA

    IPC分类号： C07K14/72 ,  C12N15/62 ,  G01N33/68

    CPC分类号： C12Q1/6813 ,  C07K16/2863 ,  C07K2319/80 ,  C12N15/1055 ,  C12N15/62 ,  C12Q1/68 ,  C12Q1/6804 ,  G01N33/6842 ,  G01N33/6845 ,  C12Q2522/101 ,  C12Q2531/113 ,  C12Q2531/125

    摘要： The invention relates to a method of detecting the coincidence of two biomolecular structures in a solid phase sample, said method comprising: (i) providing a first and a second fusion protein, each fusion protein comprising (a) a detection domain, said detection domain comprising a DNA binding domain; said detection domain capable of binding a cognate specific nucleotide sequence in co-operation with a further detection domain; (b) a recognition domain, said recognition domain capable of binding a target biomolecular structure; and (c) a connector domain; said connector domain being fused at one end to the detection domain and being fused at the other end to the recognition domain; wherein at least two of (a), (b) and (c) are heterologous to one another; wherein the recognition domains of said first and said second fusion proteins are capable of binding to first and second biomolecular structures; (ii) contacting the sample with said first and second fusion proteins; (iii) incubating to allow binding; (iv) removing unbound fusion protein; (v) contacting the sample with nucleic acid comprising said cognate specific nucleotide sequence; (vi) incubating to allow heterotrimeric binding of the nucleic acid; (vii) detecting nucleic acid bound to the sample wherein detection of nucleic acid in step; (vii) indicates that the two biomolecular structures are present coincidentally in said sample.

    摘要翻译： 本发明涉及一种检测固相样品中两种生物分子结构重合的方法，所述方法包括：（i）提供第一和第二融合蛋白，每种融合蛋白包含（a）检测结构域，所述检测结构域 包括DNA结合结构域; 所述检测结构域能够与另一检测域合用来结合同源特异性核苷酸序列; （b）识别结构域，所述识别结构域能够结合目标生物分子结构; 和（c）连接器领域; 所述连接器域在一端融合到检测域并在另一端融合到识别结构域; 其中（a），（b）和（c）中的至少两个彼此是异源的; 其中所述第一和第二融合蛋白的识别结构域能够结合第一和​​第二生物分子结构; （ii）使样品与所述第一和第二融合蛋白接触; （iii）孵育以允许结合; （iv）去除未结合的融合蛋白; （v）使样品与包含所述同源特异性核苷酸序列的核酸接触; （vi）孵育以允许核酸的异三聚体结合; （vii）检测与样品结合的核酸，其中步骤中检测核酸; （vii）表示两个生物分子结构巧合地存在于所述样品中。