公开(公告)号：WO2016195927A1

公开(公告)日：2016-12-08

申请号：PCT/US2016/031570

申请日：2016-05-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LYTLE, Steven ,  BOESCHOTEN, Paul ,  GUNDERSON, Andrew ,  RYSTROM, Larry ,  GNEHM, Chris

IPC: G02B21/00 ,  G01N21/27 ,  G01N21/64

CPC classification number: G02B21/0076 ,  G01N21/274 ,  G01N21/6458 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/0044

Abstract: A method for calibrating an imaging system, comprising: illuminating a sample through a pinhole mask using an excitation light; capturing an image of the sample using a sensor; converting the image into data; in a processing module: filtering the data using known spacing of pinholes in the pinhole mask to obtain data that corresponds to the spacing, using a threshold to identify regions of the remaining data that are bright enough to be associated with a pinhole, calculating the centroids of the regions, and fitting a known pattern for the pinhole mask to the regions in order to identify the best fit for the data; and storing, in a storage medium, the best fit data for use in a subsequent confocal capture routine.

Abstract translation: 一种用于校准成像系统的方法，包括：使用激发光照射通过针孔掩模的样品; 使用传感器捕获样品的图像; 将图像转换为数据; 在处理模块中：使用针孔掩模中的针孔的已知间距来过滤数据以获得对应于间隔的数据，使用阈值来识别足够亮以与针孔相关联的剩余数据的区域，计算质心 并且将用于针孔掩模的已知图案拟合到区域，以便识别数据的最佳拟合; 以及在存储介质中存储用于随后的共焦捕获程序中的最佳拟合数据。

公开(公告)号：WO2016195928A1

公开(公告)日：2016-12-08

申请号：PCT/US2016/031572

申请日：2016-05-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： GUNDERSON, Andrew ,  BOESCHOTEN, Paul

IPC: G02B21/00 ,  G01N21/64 ,  G02B21/26

CPC classification number: G02B21/26 ,  G01N21/6458 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/0076 ,  H02K33/00

Abstract: An apparatus for generating composite confocal images, comprising: an imaging apparatus configured to generate illumination; a sample stage configured to hold a sample to be illuminated by the illumination, wherein the illumination causes the sample to generate emissions that can be detected and used to image the sample, the sample stage configured to move the sample to a plurality of positions during the imaging process; and a vibration mechanism coupled with the sample stage, the motor configured to vibrate the stage after the stage moves the sample for a vibration period.

Abstract translation: 一种用于产生复合共焦图像的装置，包括：成像装置，被配置为产生照明; 样本台，被配置为保持要被所述照明照明的样本，其中所述照明使所述样本产生可被检测并用于对所述样本进行成像的所述发射，所述样本台被配置为在所述样本期间将所述样本移动到多个位置 成像过程; 以及与所述样品台耦合的振动机构，所述电动机构造成在所述载物台移动所述样品振动周期之后振动所述载物台。

公开(公告)号：WO2016195926A1

公开(公告)日：2016-12-08

申请号：PCT/US2016/031568

申请日：2016-05-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LYTLE, Steven ,  BOESCHOTEN, Paul ,  GUNDERSON, Andrew ,  RYSTROM, Larry ,  GNEHM, Chris

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/36

CPC classification number: G02B21/0076 ,  G01N21/645 ,  G01N21/6458 ,  G01N2021/6463 ,  G01N2021/6478 ,  G02B21/367 ,  G03F7/2004

Abstract: A method for generating a composite image obtained in a fluorescence imaging system, comprising: determining the physical locations of a pinhole mask relative to a sample required to construct a full composite confocal image; generating in a control module a randomized order for the determined physical locations; moving the photomask or the sample to the determined physical locations in the randomized order under control of the control module and using a translation stage; illuminating the sample through the photomask using an excitation light; capturing a plurality of images at each of the physical locations using a sensor in order to generate a set of data points; using the randomized order to generate a composite image based on the set of data points and measuring the brightness of at least some of the data points; and adjusting the brightness of some of the set of data points.

Abstract translation: 一种用于产生在荧光成像系统中获得的合成图像的方法，包括：确定针孔掩模相对于构建全复合共焦图像所需样品的物理位置; 在控制模块中生成用于确定的物理位置的随机化顺序; 在控制模块的控制下使用翻译阶段，以随机顺序将光掩模或样品移动到所确定的物理位置; 使用激发光照射样品通过光掩模; 使用传感器在每个物理位置捕获多个图像，以便生成一组数据点; 使用所述随机化顺序基于所述一组数据点生成合成图像并测量至少一些所述数据点的亮度; 并调整一些数据点的某些亮度。

公开(公告)号：WO2016195925A1

公开(公告)日：2016-12-08

申请号：PCT/US2016/031566

申请日：2016-05-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LYTLE, Steven ,  BOESCHOTEN, Paul ,  GUNDERSON, Andrew ,  GNEHM, Chris

IPC: G02B21/00 ,  G02B21/36

CPC classification number: G02B21/0032 ,  G02B21/0044 ,  G02B21/0056 ,  G02B21/0076 ,  G02B21/362 ,  G02B27/141

Abstract: An apparatus for generating structured illumination images, comprising a housing; imaging optics mounted within the housing, the imaging optics configured to focus illumination from an illumination source such that an in-focus pattern of light corresponding to a pattern on a photomask is projected onto a sample; a reflector mounted within the housing and configured to reflect the illumination to a sample; a filter apparatus, comprising one or more filters mounted within the housing, the filter apparatus configured to allow only emissions from the illuminated sample to pass through to the sensor; and mounting features on the housing, the mounting features configured to allow the apparatus to be installed within an imaging system.

Abstract translation: 一种用于产生结构化照明图像的装置，包括壳体; 安装在壳体内的成像光学元件，成像光学元件被配置为聚焦来自照明源的照明，使得对应于光掩模上的图案的对焦图案投射到样品上; 安装在所述壳体内并被配置为将照射反射到样品的反射器; 过滤器装置，包括安装在壳体内的一个或多个过滤器，过滤器装置被配置为仅允许来自照射样品的排放物通过传感器; 和安装特征在壳体上，安装特征被配置成允许装置安装在成像系统内。