公开(公告)号：WO2017034942A1

公开(公告)日：2017-03-02

申请号：PCT/US2016/047692

申请日：2016-08-19

Applicant: MANUS BIOSYNTHESIS, INC.

Inventor： PIRIE, Christopher ,  LI, Liwei ,  MAK, Huey-ming ,  TIBREWALA, Srishti ,  DONALD, Jason ,  KUMARAN, Ajikumar, Parayil

IPC: C12N9/00 ,  C12N5/00 ,  C12N1/21 ,  C12N5/04

CPC classification number: C12P5/007 ,  C12N9/00 ,  C12N15/52 ,  C12P19/56

Abstract: The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.

Abstract translation: 本发明涉及细菌宿主细胞中化学物质的生产。 特别地，本发明提供了功能性表达工程化的P450酶的大肠杆菌（E.coli）宿主细胞中的化学物质的生产。

公开(公告)号：WO2016073740A1

公开(公告)日：2016-05-12

申请号：PCT/US2015/059273

申请日：2015-11-05

Applicant: MANUS BIOSYNTHESIS, INC.

Inventor： PHILIPPE, Ryan ,  KUMARAN, Ajikumar Parayil ,  DONALD, Jason ,  PATEL, Krishna ,  GUPTA, Swati ,  LIM, Ryan ,  LI, Liwei

IPC: C12P19/56 ,  A23L2/60

CPC classification number: A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C12N9/0073 ,  C12N9/1048 ,  C12N9/1051 ,  C12P19/56 ,  C12Y114/13079 ,  C12Y204/01017

Abstract: The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.

Abstract translation: 本发明提供了制备甜菊糖苷的方法，其包括RebM和作为甜菊叶中次要产物的糖基化产物，并提供用于这些方法的酶，编码多核苷酸和宿主细胞。 本发明提供工程化酶和工程化宿主细胞，用于以高纯度和/或产率生产甜菊糖醇糖基化产物，例如RebM。 本发明还提供了制备含有甜菊糖苷的产品的方法，所述甜菊糖苷如RebM包括食品，饮料，口腔护理产品，甜味剂和调味产品。