公开(公告)号：WO2013141905A3

公开(公告)日：2014-03-13

申请号：PCT/US2012067216

申请日：2012-11-30

Applicant: MASCOMA CORP ,  DARTMOUTH COLLEGE ,  GUSS ADAM M

Inventor： GUSS ADAM M ,  LO JONATHAN ,  VAN DIJKEN JOHANNES P ,  SHAW IV ARTHUR J ,  OLSON DANIEL G ,  HERRING CHRISTOPHER D

IPC: C12N9/04 ,  C12N9/02 ,  C12P7/06

CPC classification number: C12P7/06 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/74 ,  Y02E50/17

Abstract: The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

Abstract translation: 本发明提供了对重组宿主细胞中辅因子使用的操作以增加所需产物的形成。 在一些实施方案中，本发明提供了包含一种或多种天然酶中的突变的重组微生物，使得它们的辅因子特异性以使得细胞中的总辅助因子在特定途径中平衡的方式改变，并且增加 细胞内的特定产物形成。 在一些实施方案中，内源性酶由具有来自不同物种的替代辅因子特异性的酶代替。

公开(公告)号：WO2013089890A3

公开(公告)日：2014-01-03

申请号：PCT/US2012057952

申请日：2012-09-28

Applicant: MASCOMA CORP ,  DARTMOUTH COLLEGE

Inventor： DENG YU ,  OLSON DANIEL G ,  VAN DIJKEN JOHANNES P ,  SHAW IV ARTHUR J ,  ARGYROS AARON ,  BARRETT TRISHA ,  CAIAZZA NICKY ,  HERRING CHRISTOPHER D ,  ROGERS STEPHEN R ,  AGBOGBO FRANK

IPC: C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12P7/06 ,  C12P7/10

CPC classification number: C12P7/065 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1294 ,  C12N9/88 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.

Abstract translation: 本发明提供对重组宿主细胞中碳通量的操作以增加所需产物的形成。 本发明涉及经过遗传修饰的纤维素消化生物，以通过在中枢代谢的关键步骤重新定向碳通量以高产率生产乙醇。