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    ENGINEERING AN INCREASE IN ETHANOL PRODUCTION BY ALTERING COFACTOR SPECIFICITY
    1.
    发明申请
    ENGINEERING AN INCREASE IN ETHANOL PRODUCTION BY ALTERING COFACTOR SPECIFICITY 审中-公开
    通过改变凝血因子特异性，在乙醇生产中工程化

    公开(公告)号：WO2013141905A3

    公开(公告)日：2014-03-13

    申请号：PCT/US2012067216

    申请日：2012-11-30

    Applicant: MASCOMA CORP ,  DARTMOUTH COLLEGE ,  GUSS ADAM M

    Inventor： GUSS ADAM M ,  LO JONATHAN ,  VAN DIJKEN JOHANNES P ,  SHAW IV ARTHUR J ,  OLSON DANIEL G ,  HERRING CHRISTOPHER D

    IPC: C12N9/04 ,  C12N9/02 ,  C12P7/06

    CPC classification number: C12P7/06 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/74 ,  Y02E50/17

    Abstract: The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

    Abstract translation: 本发明提供了对重组宿主细胞中辅因子使用的操作以增加所需产物的形成。 在一些实施方案中，本发明提供了包含一种或多种天然酶中的突变的重组微生物，使得它们的辅因子特异性以使得细胞中的总辅助因子在特定途径中平衡的方式改变，并且增加 细胞内的特定产物形成。 在一些实施方案中，内源性酶由具有来自不同物种的替代辅因子特异性的酶代替。

    PRODUCTION OF MALONYL-COA DRIVED PRODUCTS VIA ANAEROBIC PATHWAYS
    4.
    发明申请
    PRODUCTION OF MALONYL-COA DRIVED PRODUCTS VIA ANAEROBIC PATHWAYS 审中-公开
    通过厌氧途径生产MALONYL-COA驱动的产品

    公开(公告)号：WO2012019175A3

    公开(公告)日：2012-04-19

    申请号：PCT/US2011046869

    申请日：2011-08-05

    Applicant: MASCOMA CORP ,  SILLERS WILLIAM RYAN ,  TRIPATHI SHITAL A ,  SHAW IV ARTHUR J ,  ARGYROS AARON ,  HOGSETT DAVID A

    Inventor： SILLERS WILLIAM RYAN ,  TRIPATHI SHITAL A ,  SHAW IV ARTHUR J ,  ARGYROS AARON ,  HOGSETT DAVID A

    IPC: C12N9/88 ,  C12N1/22 ,  C12N9/10 ,  C12P5/00 ,  C12Q1/48 ,  D21C5/00

    CPC classification number: C12P17/182 ,  C12N1/22 ,  C12N9/1018 ,  C12N9/88 ,  C12P7/6409 ,  G01N2333/91034

    Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.

    Abstract translation: 本发明提供了新的代谢途径，以在厌氧条件下和在ATP的净产生下将生物质和其他碳水化合物源转化为丙二酰-CoA衍生的产物如烃和其他生物产物。 更具体地说，本发明提供了包含一种或多种天然和/或异源酶的重组微生物，所述天然和/或异源酶在一种或多种工程化代谢途径中起作用以实现碳水化合物源向例如长链烃和烃衍生物的转化， 一种或多种天然和/或异源酶被激活，上调，下调或缺失。 本发明还提供了将生物质转化为丙二酰-CoA衍生产物的方法，其包括使碳水化合物源与本发明的重组微生物接触。

    PRODUCTION OF MALONYL-COA DERIVED PRODUCTS VIA ANAEROBIC PATHWAYS
    5.
    发明申请
    PRODUCTION OF MALONYL-COA DERIVED PRODUCTS VIA ANAEROBIC PATHWAYS 审中-公开
    通过厌氧途径生产马来酰亚胺衍生产品

    公开(公告)号：WO2012019175A8

    公开(公告)日：2012-08-30

    申请号：PCT/US2011046869

    申请日：2011-08-05

    Applicant: MASCOMA CORP ,  SILLERS WILLIAM RYAN ,  TRIPATHI SHITAL A ,  SHAW IV ARTHUR J ,  ARGYROS AARON ,  HOGSETT DAVID A

    Inventor： SILLERS WILLIAM RYAN ,  TRIPATHI SHITAL A ,  SHAW IV ARTHUR J ,  ARGYROS AARON ,  HOGSETT DAVID A

    IPC: C12N9/88 ,  C12N1/22 ,  C12N9/10 ,  C12P5/00 ,  C12Q1/48 ,  D21C5/00

    CPC classification number: C12P17/182 ,  C12N1/22 ,  C12N9/1018 ,  C12N9/88 ,  C12P7/6409 ,  G01N2333/91034

    Abstract: The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.

    Abstract translation: 本发明提供了在厌氧条件下和ATP的净产生下将生物质和其他碳水化合物来源转化成丙二酰辅酶A衍生产物如烃和其他生物产物的新型代谢途径。 更具体地，本发明提供了一种重组微生物，其包含在一个或多个工程化代谢途径中起作用以实现碳水化合物源转化为例如长链烃和烃衍生物的一种或多种天然和/或异源酶，其中 一种或多种天然和/或异源酶被激活，上调，下调或缺失。 本发明还提供将生物质转化为丙二酰辅酶A衍生产物的方法，其包括使碳水化合物源与本发明的重组微生物接触。

    HETEROLOGOUS BIOMASS DEGRADING ENZYME EXPRESSION IN THERMOANAEROBACTERIUM SACCHAROLYTICUM
    6.
    发明申请
    HETEROLOGOUS BIOMASS DEGRADING ENZYME EXPRESSION IN THERMOANAEROBACTERIUM SACCHAROLYTICUM 审中-公开
    热酵母菌中异源生物量降解酶的表达

    公开(公告)号：WO2010075529A3

    公开(公告)日：2010-08-19

    申请号：PCT/US2009069443

    申请日：2009-12-23

    Applicant: MASCOMA CORP ,  HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

    Inventor： HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

    IPC: C12N15/74 ,  C12P21/00

    CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

    Abstract: Thermophilic gram-positive anaerobic host cells, for example Themoanaerobactenum sachharolyticum ("T sacch"), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2 In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide In addition, the host cell, for example, a T sacch. host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol

    Abstract translation: 嗜热革兰氏阳性厌氧宿主细胞，例如莎草精酵母（“T sacch”）表达异源生物降解酶，例如纤维素酶，并且能够从纤维素生产有用的发酵产物有用的发酵产物包括例如乙醇，乙酸 酸，乳酸或CO 2为了提供最大的表达和活性水平，生物降解酶可以从密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与 信号肽另外，宿主细胞，例如T细胞。 宿主细胞，可进行遗传改造以进一步改善乙醇生产，例如通过破坏乙醇以外的有机产物的生产

    METHOD FOR ACETATE CONSUMPTION DURING ETHANOLIC FERMENTATION OF CELLULOSIC FEEDSTOCKS
    7.
    发明申请
    METHOD FOR ACETATE CONSUMPTION DURING ETHANOLIC FERMENTATION OF CELLULOSIC FEEDSTOCKS 审中-公开
    纤维素饲料乙醇发酵过程中乙酸消费的方法

    公开(公告)号：WO2014074895A2

    公开(公告)日：2014-05-15

    申请号：PCT/US2013069266

    申请日：2013-11-08

    Applicant: MASCOMA CORP

    Inventor： ZELLE RINTZE MEINDERT ,  SHAW IV ARTHUR J ,  VAN DIJKEN JOHANNES PIETER

    IPC: C12N9/04 ,  C12N9/02 ,  C12P7/10

    CPC classification number: C12P7/10 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/52 ,  C12N15/81 ,  C12P7/06 ,  C12Y101/01175 ,  C12Y101/01307 ,  C12Y102/0101 ,  Y02E50/16 ,  Y02E50/17

    Abstract: The present invention provides for novel metabolic pathways to detoxify biomass- derived acetate via metabolic conversion to ethanol, acetone, or isopropanol. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more first engineered metabolic pathways to achieve: (1) conversion of acetate to ethanol; (2) conversion of acetate to acetone; or (3) conversion of acetate to isopropanol; and one or more native and/or heterologous enzymes that function in one or more second engineered metabolic pathways to produce an electron donor used in the conversion of acetate to less inhibitory compounds; wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated.

    Abstract translation: 本发明提供了通过代谢转化为乙醇，丙酮或异丙醇对生物质衍生的乙酸盐进行解毒的新型代谢途径。 更具体地，本发明提供了一种重组微生物，其包含在一个或多个第一工程化代谢途径中起作用的一种或多种天然和/或异源酶，以实现：（1）将乙酸酯转化为乙醇; （2）乙酸酯转化为丙酮; 或（3）将乙酸酯转化成异丙醇; 和一种或多种在一种或多种第二工程化代谢途径中起作用的天然和/或异源酶，以产生用于将乙酸转化为较少抑制性化合物的电子给体; 其中所述一种或多种天然和/或异源酶被激活，上调或下调。

    HETEROLOGOUS BIOMASS DEGRADING ENZYME EXPRESSION IN THERMOANAEROBACTERIUM SACCHAROLYTICUM
    8.
    发明申请
    HETEROLOGOUS BIOMASS DEGRADING ENZYME EXPRESSION IN THERMOANAEROBACTERIUM SACCHAROLYTICUM 审中-公开
    热酵母菌中异源生物量降解酶的表达

    公开(公告)号：WO2010075529A9

    公开(公告)日：2011-01-13

    申请号：PCT/US2009069443

    申请日：2009-12-23

    Applicant: MASCOMA CORP ,  HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

    Inventor： HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

    IPC: C12N15/74 ,  C12P21/00

    CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

    Abstract: Thermophilic gram-positive anaerobic host cells, for example Themoanaerobacterium sachharolyticum ("T sacch"), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

    Abstract translation: 嗜热革兰氏阳性厌氧宿主细胞，例如沙拉梭菌（“茶糖酵母”）表达异源生物降解酶，如纤维素酶，并能够从纤维素中产生有用的发酵产物。 有用的发酵产物包括例如乙醇，乙酸，乳酸或二氧化碳。 为了提供最大的表达和活性水平，生物降解酶可以由密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与信号肽融合。 此外，宿主细胞，例如T细胞宿主细胞，可进行遗传改变以进一步改善乙醇生产，例如通过破坏除乙醇以外的有机产物的生产。

    GENE KNOCKOUT MESOPHILIC AND THERMOPHILIC ORGANISMS, AND METHODS OF USE THEREOF
    9.
    发明申请
    GENE KNOCKOUT MESOPHILIC AND THERMOPHILIC ORGANISMS, AND METHODS OF USE THEREOF 审中-公开
    基因克隆夫妇和热休克有机体及其使用方法

    公开(公告)号：WO2010056805A3

    公开(公告)日：2010-07-08

    申请号：PCT/US2009064128

    申请日：2009-11-12

    Applicant: MASCOMA CORP ,  HOGSETT DAVID A ,  RAJGARHIA VINEET ,  SHAW IV ARTHUR J ,  CAIAZZA NICKY C

    Inventor： HOGSETT DAVID A ,  RAJGARHIA VINEET ,  SHAW IV ARTHUR J ,  CAIAZZA NICKY C

    IPC: C07H21/02 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12N15/00 ,  C12P7/06 ,  C12R1/01

    CPC classification number: C12P7/54 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12P7/065 ,  C12P7/56 ,  Y02E50/17

    Abstract: One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

    Abstract translation: 本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，所述第一天然基因编码参与 代谢产生有机酸或其盐，从而增加所述嗜热或嗜温微生物产生作为发酵产物的乳酸盐或乙酸盐的天然能力。 在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乳酸盐或乙酸盐的代谢产生的第一非天然酶。 本发明的另一方面涉及将木质纤维素生物质转化为乳酸盐或乙酸盐的方法，包括使木质纤维素生物质与遗传修饰的嗜热或嗜温微生物接触。

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