公开(公告)号：WO2013141905A3

公开(公告)日：2014-03-13

申请号：PCT/US2012067216

申请日：2012-11-30

Applicant: MASCOMA CORP ,  DARTMOUTH COLLEGE ,  GUSS ADAM M

Inventor： GUSS ADAM M ,  LO JONATHAN ,  VAN DIJKEN JOHANNES P ,  SHAW IV ARTHUR J ,  OLSON DANIEL G ,  HERRING CHRISTOPHER D

IPC: C12N9/04 ,  C12N9/02 ,  C12P7/06

CPC classification number: C12P7/06 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/74 ,  Y02E50/17

Abstract: The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

Abstract translation: 本发明提供了对重组宿主细胞中辅因子使用的操作以增加所需产物的形成。 在一些实施方案中，本发明提供了包含一种或多种天然酶中的突变的重组微生物，使得它们的辅因子特异性以使得细胞中的总辅助因子在特定途径中平衡的方式改变，并且增加 细胞内的特定产物形成。 在一些实施方案中，内源性酶由具有来自不同物种的替代辅因子特异性的酶代替。

公开(公告)号：WO2013089890A3

公开(公告)日：2014-01-03

申请号：PCT/US2012057952

申请日：2012-09-28

Applicant: MASCOMA CORP ,  DARTMOUTH COLLEGE

Inventor： DENG YU ,  OLSON DANIEL G ,  VAN DIJKEN JOHANNES P ,  SHAW IV ARTHUR J ,  ARGYROS AARON ,  BARRETT TRISHA ,  CAIAZZA NICKY ,  HERRING CHRISTOPHER D ,  ROGERS STEPHEN R ,  AGBOGBO FRANK

IPC: C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12P7/06 ,  C12P7/10

CPC classification number: C12P7/065 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/1294 ,  C12N9/88 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.

Abstract translation: 本发明提供对重组宿主细胞中碳通量的操作以增加所需产物的形成。 本发明涉及经过遗传修饰的纤维素消化生物，以通过在中枢代谢的关键步骤重新定向碳通量以高产率生产乙醇。

公开(公告)号：WO2010008578A3

公开(公告)日：2010-03-25

申请号：PCT/US2009004135

申请日：2009-07-17

Applicant: MASCOMA CORP ,  HERRING CHRISTOPHER D ,  LIU CHAOGANG ,  BARDSLEY JOHN

Inventor： HERRING CHRISTOPHER D ,  LIU CHAOGANG ,  BARDSLEY JOHN

IPC: C12P7/10 ,  C08B1/00 ,  C12P19/14 ,  C13K1/02 ,  D21C3/22

CPC classification number: C12P7/10 ,  C08B37/0003 ,  C08B37/0057 ,  C08H8/00 ,  C12M21/12 ,  C12M27/02 ,  C12M29/04 ,  C12M45/09 ,  C12P19/14 ,  C13K1/02 ,  D21C3/22 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.

Abstract translation: 本发明涉及从木质纤维素生物质生物转化碳水化合物的方法，其包括以下步骤：将木质纤维素生物质悬浮在流通式反应器中，使反应溶液进入反应器中，其中所述溶液被吸收到生物质基质中，并且至少 一部分溶液通过所述生物质基质迁移到液体储存器中，使液体储存器中的反应溶液再循环至少一次以再次被吸收并迁移通过生物质基质。 所述生物转化可涉及水解纤维素，半纤维素或其组合以形成寡糖，单体糖或其组合; 发酵低聚糖，单体糖或其组合以产生乙醇或其组合。 该方法可进一步包括除去反应溶液并处理溶液以分离由非发酵固体产生的乙醇。