公开(公告)号：WO2016004536A1

公开(公告)日：2016-01-14

申请号：PCT/CA2015/050644

申请日：2015-07-10

Applicant: MEDICAGO INC. ,  UNIVERSITE LAVAL

Inventor： MICHAUD, Dominique ,  PEPIN, Steeve ,  ETHIER, Gilbert ,  GOULET, Marie-Claire ,  GAUDREAU, Linda ,  GAGNE, Marielle ,  MARTEL, Michele ,  BECHTOLD, Nicole ,  D'AOUST, Marc-Andre ,  GOSSELIN, Andre

IPC: C12N15/00 ,  A01H3/00 ,  A01H5/00 ,  C12N15/82 ,  C12P21/00

CPC classification number: C12N15/8251 ,  A01H3/00 ,  A01H3/02 ,  A01H3/04 ,  C12N15/8258

Abstract: A method for synthesizing a protein of interest within a plant or a portion of a plant is provided. The method involves treating the plant to increase secondary leaf biomass production, followed by introducing one or more than one nucleic acid sequence encoding a protein of interest operatively linked with a regulatory region that is active in the plant, into the plant. The plant is then maintained under conditions that permit the nucleic acid sequence encoding the protein of interest to be expressed in the plant or the portion of the plant. Optionally, the plant or portion of the plant may be harvested and the protein of interest extracted.

Abstract translation: 提供了在植物或植物的一部分内合成感兴趣的蛋白质的方法。 该方法包括处理植物以增加次生叶生物量的产生，随后将编码目的蛋白质的一种或多于一种的核酸序列与在植物中具有活性的调节区域可操作地连接到植物中。 然后将植物保持在允许编码目的蛋白质的核酸序列在植物或植物部分中表达的条件下。 任选地，可以收获植物或植物的一部分，并提取目的蛋白质。