公开(公告)号：WO2016004536A1

公开(公告)日：2016-01-14

申请号：PCT/CA2015/050644

申请日：2015-07-10

Applicant: MEDICAGO INC. ,  UNIVERSITE LAVAL

Inventor： MICHAUD, Dominique ,  PEPIN, Steeve ,  ETHIER, Gilbert ,  GOULET, Marie-Claire ,  GAUDREAU, Linda ,  GAGNE, Marielle ,  MARTEL, Michele ,  BECHTOLD, Nicole ,  D'AOUST, Marc-Andre ,  GOSSELIN, Andre

IPC: C12N15/00 ,  A01H3/00 ,  A01H5/00 ,  C12N15/82 ,  C12P21/00

CPC classification number: C12N15/8251 ,  A01H3/00 ,  A01H3/02 ,  A01H3/04 ,  C12N15/8258

Abstract: A method for synthesizing a protein of interest within a plant or a portion of a plant is provided. The method involves treating the plant to increase secondary leaf biomass production, followed by introducing one or more than one nucleic acid sequence encoding a protein of interest operatively linked with a regulatory region that is active in the plant, into the plant. The plant is then maintained under conditions that permit the nucleic acid sequence encoding the protein of interest to be expressed in the plant or the portion of the plant. Optionally, the plant or portion of the plant may be harvested and the protein of interest extracted.

Abstract translation: 提供了在植物或植物的一部分内合成感兴趣的蛋白质的方法。 该方法包括处理植物以增加次生叶生物量的产生，随后将编码目的蛋白质的一种或多于一种的核酸序列与在植物中具有活性的调节区域可操作地连接到植物中。 然后将植物保持在允许编码目的蛋白质的核酸序列在植物或植物部分中表达的条件下。 任选地，可以收获植物或植物的一部分，并提取目的蛋白质。

公开(公告)号：WO2008151444A1

公开(公告)日：2008-12-18

申请号：PCT/CA2008/001146

申请日：2008-06-13

Applicant: MEDICAGO INC. ,  D'AOUST, Marc-Andre ,  BELLES-ISLES, Julie ,  BECHTOLD, Nicole ,  MARTEL, Michele ,  LAVOIE, Pierre-Olivier ,  VEZINA, Louis-Philippe

Inventor： D'AOUST, Marc-Andre ,  BELLES-ISLES, Julie ,  BECHTOLD, Nicole ,  MARTEL, Michele ,  LAVOIE, Pierre-Olivier ,  VEZINA, Louis-Philippe

IPC: C12N15/82 ,  C12N15/09 ,  C12N15/87 ,  C12P21/02 ,  C12N15/29 ,  A01H5/00

CPC classification number: C12N15/8257 ,  A01H3/00 ,  A01H3/04 ,  C12N9/1051 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8258

Abstract: A method for synthesizing a protein of interest within a plant or a portion of a plant is provided. The method involves introducing one or more than one nucleic acid sequence encoding a protein of interest operatively linked with a regulatory region obtained from a photosynthetic gene that is active in the plant, in a transient manner. The plant is then maintained under conditions that permit the nucleic acid sequence encoding the protein of interest to be expressed in the plant or the portion of the plant. The plant may be pruned prior to the introducing one or more than one nucleic acid sequence.

Abstract translation: 提供了在植物或植物的一部分内合成感兴趣的蛋白质的方法。 该方法包括以瞬时方式引入编码目的蛋白质的一种或多于一种的核酸序列，其与从植物中活性的光合基因获得的调节区域可操作地连接。 然后将植物保持在允许编码目的蛋白质的核酸序列在植物或植物部分中表达的条件下。 可以在引入一个或多于一个核酸序列之前修剪植物。

公开(公告)号：WO2013120204A1

公开(公告)日：2013-08-22

申请号：PCT/CA2013/050118

申请日：2013-02-15

Applicant: MEDICAGO INC.

Inventor： D'AOUST, Marc-André ,  BECHTOLD, Nicole ,  LAURIN, Luc ,  VEZINA, Louis-Philippe ,  DUBUC, Normand

IPC: C12M1/26 ,  A01H5/00 ,  C12M3/00 ,  C12N15/87 ,  A01G7/00 ,  C12N15/82

CPC classification number: A01G7/06 ,  A01G7/00 ,  A01G22/00 ,  C12N15/8205

Abstract: It is provided a plant infiltration device for infiltrating plants with an inoculum and method of using same, the device comprising a frame having one or more tray racks adapted for receiving at least one tray of plants to be infiltrated; an automated manipulation mechanism mounted to the frame and operable to displace the plant trays within the device; a plurality of inoculum reservoirs mounted to the frame and containing one or more inoculum fluids; an infiltration tank mounted to the frame and within which the plant trays are received when disposed in the infiltration position; and a vacuum generating device in communication with the infiltration tank to apply a negative pressure within the infiltration tank, thereby opening pores of the plant leaves immersed in the inoculum within the infiltration tank and causing the inoculum fluid to infiltrate into the plant leaves.

Abstract translation: 提供了一种用于用接种物渗透植物的植物浸渗装置及其使用方法，所述装置包括具有一个或多个适于接收至少一个待渗透的植物盘的一个或多个托盘架的框架; 安装到所述框架并且可操作以移动所述装置内的所述植物托盘的自动操作机构; 多个接种物贮存器，其安装在框架上并含有一种或多种接种液; 安装在所述框架上并且当设置在所述渗透位置时所述植物托盘被接收的渗透罐; 以及与渗透槽连通以在渗透槽内施加负压的真空产生装置，从而打开浸入渗透罐内的接种物中的植物叶片的孔，并使接种液渗入植物叶中。

公开(公告)号：WO2013177701A1

公开(公告)日：2013-12-05

申请号：PCT/CA2013/050406

申请日：2013-05-28

Applicant: MEDICAGO INC.

Inventor： VÉZINA, Louis-Philippe ,  BECHTOLD, Nicole ,  LAURIN, Luc

IPC: A01G9/02

CPC classification number: A01G9/02 ,  A01G9/0297

Abstract: A plant tray for receiving and retaining one or more plant pots includes a plant- receiving tray for use in a high-throughput plant infiltration process used to produce recombinant proteins. The plant tray described has a base retaining tray with an upper surface defining cavities for receiving the plant pots, and a top plate displaceably mounted on the base retaining tray and having apertures, the apertures defining a channel with the corresponding cavities of the bottom tray when aligned. The top plate is displaceable between a locked operative position wherein the apertures and the corresponding cavities are partially misaligned, thereby locking the plant pots in between the top plate and the base retaining tray.

Abstract translation: 用于接收和保留一个或多个植物盆的植物托盘包括用于生产重组蛋白质的高通量植物浸润过程中的植物接收盘。 所描述的植物托盘具有基部保持托盘，其具有限定用于容纳植物盆的空腔的上表面，以及可移动地安装在基部保持托盘上并具有孔的顶板，所述孔限定与底托盘的相应空腔的通道， 对齐。 顶板可在锁定的操作位置之间移动，其中孔和相应的空腔部分地不对准，从而将植物盆锁定在顶板和基座保持盘之间。