公开(公告)号：WO1998045406A1

公开(公告)日：1998-10-15

申请号：PCT/US1997014134

申请日：1997-08-08

Applicant: MINNESOTA MINING AND MANUFACTURING COMPANY

Inventor： MINNESOTA MINING AND MANUFACTURING COMPANY ,  WILLIAMS, Michael, G. ,  HALVERSON, Kurt, J. ,  KREJCAREK, Gary, E. ,  WEI, Ai-Ping ,  BERG, James, G. ,  WICKERT, Peter, D. ,  CALHOUN, Clyde, D. ,  DEBE, Mark, K. ,  QIU, Jun

IPC: C12M01/20

CPC classification number: B01L3/5085 ,  B01L3/5088 ,  B01L2300/0893 ,  C12M23/04 ,  C12M25/01 ,  C12M41/36

Abstract: A method for partitioning an aqueous biological liquid sample into discrete microvolumes for detection and enumeration of microorganisms is described. The method involves distributing microvolumes of a sample to a plurality of hydrophilic liquid-retaining zones of a culture device, where each liquid-retaining zone is surrounded by a portion of a hydrophobic "land" area. Also disclosed are devices for carrying out these methods.

Abstract translation: 描述了将水性生物液体样品分成用于微生物检测和计数的离散微量体积的方法。 该方法包括将样品的微量体积分配到培养装置的多个亲水性液体保持区，其中每个液体保持区被疏水性“陆地”区域的一部分包围。 还公开了用于执行这些方法的装置。