公开(公告)号：WO1998050579A1

公开(公告)日：1998-11-12

申请号：PCT/US1997016579

申请日：1997-09-08

Applicant: MINNESOTA MINING AND MANUFACTURING COMPANY

Inventor： MINNESOTA MINING AND MANUFACTURING COMPANY ,  WEI, Ai-Ping ,  WILLIAMS, Michael, G.

IPC: C12Q01/37

CPC classification number: C07K7/06 ,  C12Q1/37 ,  C12Q2337/40 ,  Y02A50/451

Abstract: A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a peptide, the dye groups being of proximity sufficiently close so as to essentially self-quench fluorescence of the dye groups, wherein self-quenching of fluorescence of the dye groups is effected by dye stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye stacking, and producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.

Abstract translation: 一种生物测定方法包括以下步骤：提供包含与肽结合的两个荧光染料基团的酶底物，所述染料基团接近度足够接近，从而基本上自发淬灭染料基团的荧光，其中荧光自熄 的染料组合通过染料层叠实现，并且酶切割肽以从染料堆叠中释放荧光染料基团，并产生荧光强度的增加。 还公开了用于本发明方法的蛋白酶底物。 本发明用于微生物的检测和鉴定，灭菌保证，药物发现，酶测定，免疫测定和其他生物测定。

公开(公告)号：WO1998045406A1

公开(公告)日：1998-10-15

申请号：PCT/US1997014134

申请日：1997-08-08

Applicant: MINNESOTA MINING AND MANUFACTURING COMPANY

Inventor： MINNESOTA MINING AND MANUFACTURING COMPANY ,  WILLIAMS, Michael, G. ,  HALVERSON, Kurt, J. ,  KREJCAREK, Gary, E. ,  WEI, Ai-Ping ,  BERG, James, G. ,  WICKERT, Peter, D. ,  CALHOUN, Clyde, D. ,  DEBE, Mark, K. ,  QIU, Jun

IPC: C12M01/20

CPC classification number: B01L3/5085 ,  B01L3/5088 ,  B01L2300/0893 ,  C12M23/04 ,  C12M25/01 ,  C12M41/36

Abstract: A method for partitioning an aqueous biological liquid sample into discrete microvolumes for detection and enumeration of microorganisms is described. The method involves distributing microvolumes of a sample to a plurality of hydrophilic liquid-retaining zones of a culture device, where each liquid-retaining zone is surrounded by a portion of a hydrophobic "land" area. Also disclosed are devices for carrying out these methods.

Abstract translation: 描述了将水性生物液体样品分成用于微生物检测和计数的离散微量体积的方法。 该方法包括将样品的微量体积分配到培养装置的多个亲水性液体保持区，其中每个液体保持区被疏水性“陆地”区域的一部分包围。 还公开了用于执行这些方法的装置。