公开(公告)号：WO2021183827A2

公开(公告)日：2021-09-16

申请号：PCT/US2021/022002

申请日：2021-03-11

Applicant: NATURE TECHNOLOGY CORPORATION

Inventor： WILLIAMS, James, A.

IPC: C12N15/70 ,  C12N1/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21

Abstract: The present disclosure provides engineered E. coli host cells that combine a knockout of SbcC, SbcD, or both without certain other mutations that can be used to propogate vectors. Methods of improved vector production using such engineered E. coli host cells are also provided.

公开(公告)号：WO2008153733A2

公开(公告)日：2008-12-18

申请号：PCT/US2008/006554

申请日：2008-05-22

Applicant: NATURE TECHNOLOGY CORPORATION ,  WILLIAMS, James, A.

Inventor： WILLIAMS, James, A.

IPC: A61K48/00

CPC classification number: A61K48/0066 ,  A61K39/12 ,  A61K39/145 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55561 ,  C07K14/005 ,  C12N15/111 ,  C12N15/113 ,  C12N15/117 ,  C12N15/85 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/17 ,  C12N2310/532 ,  C12N2320/30 ,  C12N2320/50 ,  C12N2710/10322 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2800/106 ,  C12N2800/107 ,  C12N2830/00

Abstract: Improved DNA vaccine plasmids are disclosed that contain novel immunostimulatory RNA compositions. The improved plasmids eliminate all extraneous sequences, incorporate a novel antibiotic free short RNA based selectable marker, increase eukaryotic expression using a novel chimeric promoter, improve yield and stability during bacterial production, and improve immunostimulation. These vectors are utilized in immunization to elicit improved immune responses or therapy to induce type 1 interferon production.

Abstract translation: 公开了包含新型免疫刺激RNA组合物的改进的DNA疫苗质粒。 改进的质粒消除所有外来序列，并结合一种新型的基于抗生素的短基于RNA的可选择标记，使用新型嵌合启动子增加真核表达，提高细菌生产过程中的产量和稳定性，并改善免疫刺激。 这些载体用于免疫以引发改善的免疫应答或诱导1型干扰素生产的治疗。

公开(公告)号：WO2008153731A1

公开(公告)日：2008-12-18

申请号：PCT/US2008/006548

申请日：2008-05-22

Applicant: NATURE TECHNOLOGY CORPORATION ,  WILLIAMS, James, A.

Inventor： WILLIAMS, James, A.

IPC: C12N1/00 ,  C12N15/90

CPC classification number: C12N15/902 ,  C12N15/1079

Abstract: Improvements in strain engineering technology are needed to insure the economic feasibility of future engineered recombinant organisms for industrial biotechnology. Disclosed herein are rapid, efficient methods (Genome Mass Transfer) that facilitate introduction of new selectable traits into a target microbial host. In one preferred embodiment, methods for high efficiency electroporation mediated transfer of donor DNA into a recipient microbial cell are disclosed.

Abstract translation: 需要改进应变工程技术，以确保未来工程重组生物工业生物技术的经济可行性。 本文公开了有助于将新的可选性状引入目标微生物宿主中的快速，有效的方法（Genome Mass Transfer）。 在一个优选的实施方案中，公开了用于高效电穿孔介导的供体DNA转移到受体微生物细胞中的方法。

公开(公告)号：WO2022056291A1

公开(公告)日：2022-03-17

申请号：PCT/US2021/049914

申请日：2021-09-10

Applicant: NATURE TECHNOLOGY CORPORATION

Inventor： WILLIAMS, James, A.

IPC: C12N15/86

Abstract: The present disclosure provides novel combinations of muscle-specific enhancers and promoters useful for achieving high and persistent expression in muscle tissue or myocytes. The muscle- specific promoter elements are derived from a desmin promoter. The muscle-specific enhancer elements are derived from a desmin promoter and a muscle creatine kinase enhancer.

公开(公告)号：WO2014077866A8

公开(公告)日：2014-05-22

申请号：PCT/US2013/000259

申请日：2013-11-18

Applicant: NATURE TECHNOLOGY CORPORATION

Inventor： WILLIAMS, James, A.

IPC: C12N15/63

Abstract: The present invention relates to the production and use of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve expression of said DNA molecules.

公开(公告)号：WO2014077866A1

公开(公告)日：2014-05-22

申请号：PCT/US2013/000259

申请日：2013-11-18

Applicant: NATURE TECHNOLOGY CORPORATION

Inventor： WILLIAMS, James, A.

IPC: C12N15/63

CPC classification number: C12N15/85 ,  A61K48/00 ,  C12N15/63 ,  C12N15/64 ,  C12N2800/107 ,  C12N2800/24 ,  C12N2820/55 ,  C12N2830/42 ,  C12P19/34 ,  C12P21/00

Abstract: The present invention relates to the production and use of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve expression of said DNA molecules.

Abstract translation: 本发明涉及共价闭合的环状（ccc）重组DNA分子如质粒，粘粒，细菌人造染色体（BAC），噬菌体，病毒载体及其杂合物的生产和使用，更具体地涉及改进表达的载体修饰 说DNA分子。

公开(公告)号：WO2014077863A1

公开(公告)日：2014-05-22

申请号：PCT/US2013/000067

申请日：2013-03-14

Applicant: NATURE TECHNOLOGY CORPORATION ,  WILLIAMS, James, A.

Inventor： WILLIAMS, James, A.

IPC: C12N15/63

CPC classification number: C12N15/85 ,  A61K48/00 ,  C12N15/63 ,  C12N15/64 ,  C12N2800/107 ,  C12N2800/24 ,  C12N2820/55 ,  C12N2830/42 ,  C12P19/34 ,  C12P21/00

Abstract: The present invention relates to the production and use of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve expression of said DNA molecules.

Abstract translation: 本发明涉及共价闭合环状（ccc）重组DNA分子如质粒，粘粒，细菌人工染色体（BAC），噬菌体，病毒载体及其杂交体等的生产和用途 特别涉及改善所述DNA分子表达的载体修饰。

公开(公告)号：WO2014035457A1

公开(公告)日：2014-03-06

申请号：PCT/US2013/000068

申请日：2013-03-14

Applicant: NATURE TECHNOLOGY CORPORATION ,  WILLIAMS, James, A.

Inventor： WILLIAMS, James, A.

IPC: A61K48/00 ,  C12N15/85

CPC classification number: C12N15/635 ,  A61K39/00 ,  A61K48/00 ,  C12N15/70 ,  C12N15/85 ,  C12N2800/107 ,  C12N2820/55

Abstract: The present invention relates to the production and use of covalently closed circular (ccc) recombinant plasmids, and more particularly to vector modifications that improve expression of said DNA molecules in the target organism.

Abstract translation: 本发明涉及共价闭合的环状（ccc）重组质粒的生产和使用，更具体地涉及改善靶生物体内所述DNA分子表达的载体修饰。

公开(公告)号：WO2009025690A2

公开(公告)日：2009-02-26

申请号：PCT/US2008/006553

申请日：2008-05-22

Applicant: NATURE TECHNOLOGY CORPORATION ,  WILLIAMS, James, A.

Inventor： WILLIAMS, James, A. ,  HODGSON, Clague, P. ,  CARNES, Aaron, E.

CPC classification number: C12N15/64 ,  C12N1/06 ,  C12N15/1003 ,  C12N15/1017 ,  C12N15/73

Abstract: General methods and strains of bacteria are described, that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.

Abstract translation: 描述了一般方法和细菌菌株，其显着简化和简化质粒DNA生产。 在一个优选的实施方案中，细胞内溶素介导的质粒提取结合絮凝介导的细胞碎片和宿主核酸的去除通过简化的下游纯化和减少的废物流实现了增加的产量和纯度，从而降低了生产成本。