公开(公告)号：WO2015138034A2

公开(公告)日：2015-09-17

申请号：PCT/US2014/071611

申请日：2014-12-19

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： INGBER, Donald E. ,  HAJIPOURAN BENAM, Kambez ,  VILLENAVE, Remi ,  HAMILTON, Geraldine A. ,  HASSELL, Bryan ,  HINOJOSA, Christopher D.

IPC: C12M3/02

CPC classification number: G01N33/5044 ,  B01L3/5027 ,  C12M21/08 ,  C12M23/16 ,  C12M25/02 ,  C12M35/04 ,  C12M41/46 ,  G01N2800/122

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

Abstract translation: 本文提供了用于生产和使用具有被一个或多个膜分隔开的中央通道的主体的系统和方法。 膜（一个或多个）被配置为将中央通道分成至少一个中途通道和至少一个微通道。 中通道的高度实质上大于微通道的高度。 气态流体可以通过中孔道施加，而液态流体流过微型通道。 本文描述的系统和方法可以用于各种应用，包括例如原代细胞如人肺细胞的生长和分化，以及需要低剪切和/或分层结构的任何其他细胞，或者模拟微环境 活体组织和/或器官（以模拟生理或疾病状态，和/或识别治疗剂和/或疫苗）。 该系统和方法还可以允许与一种或多种不同细胞类型共培养。

公开(公告)号：WO2014210339A1

公开(公告)日：2014-12-31

申请号：PCT/US2014/044381

申请日：2014-06-26

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： INGBER, Donald E. ,  LEVNER, Daniel ,  THOMPSON, Guy ,  HINOJOSA, Christopher D. ,  FERNANDEZ-ALCON, Jose

IPC: G02B21/24

CPC classification number: G02B21/24 ,  G02B7/04 ,  G02B21/02 ,  G02B21/06 ,  G02B21/14 ,  G02B21/16 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36

Abstract: A microscopy system for monitoring of one or more specimens includes a plurality of microscope blades, each microscope blade having at least one objective, at least one illuminator, and at least one detector. The microscopy system also includes a plurality of carriages, each carriage being connected to one or more of the microscope blades, and one or more actuators configured to drive the plurality of carriages along one or more axes, at least some of the plurality of carriages having at least partially overlapping ranges of motion along at least one of the one or more axes. The microscopy system also includes a master controller configured to drive each of the carriages, using the actuator(s), along the one or more axes.

Abstract translation: 用于监测一个或多个标本的显微镜系统包括多个显微镜刀片，每个显微镜刀片具有至少一个物镜，至少一个照明器和至少一个检测器。 显微镜系统还包括多个托架，每个托架连接到一个或多个显微镜刀片，以及一个或多个致动器，构造成沿着一个或多个轴线驱动多个托架，多个托架中的至少一些托架具有 沿所述一个或多个轴中的至少一个轴线的至少部分重叠的运动范围。 显微镜系统还包括主控制器，该主控制器被配置成沿着一个或多个轴使用致动器驱动每个托架。