公开(公告)号：WO2019067383A1

公开(公告)日：2019-04-04

申请号：PCT/US2018/052525

申请日：2018-09-25

Applicant: PURDUE RESEARCH FOUNDATION ,  SUMITOMO CHEMICAL COMPANY LIMITED

Inventor： WEI, Alexander ,  YUAN, Chongli

IPC: C12Q1/68 ,  C12N15/115 ,  C40B40/06

CPC classification number: C12N15/1093 ,  C12N15/111 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q1/6811 ,  C40B40/06 ,  C12Q2525/205 ,  C12Q2563/149

Abstract: The present disclosure relates to aptamers for detection of an analyte in a sample; a substrate having attached thereto the aptamers according to the invention; a kit of parts for the detection of an analyte in a sample; and a method for the detection of an analyte in a sample comprising the use of said aptamers.

公开(公告)号：WO2018081193A1

公开(公告)日：2018-05-03

申请号：PCT/US2017/058177

申请日：2017-10-25

Applicant: PURDUE RESEARCH FOUNDATION

Inventor： WEI, Alexander ,  YUAN, Chongli

IPC: C07H21/04 ,  C12N15/10 ,  C12N15/115 ,  C12Q1/68 ,  G01N33/53

Abstract: The present disclosure relates to aptamer-binding partner conjugates for detection of an analyte in a sample; a substrate having attached thereto the aptamer-binding partner conjugate according to the invention; a method for the detection of an analyte in a sample comprising use of said aptamer-binding partner conjugate; and a binding partner for conjugating to an aptamer to generate an aptamer-binding partner conjugate.

Abstract translation: 本公开涉及用于检测样品中的分析物的适体 - 结合配偶体缀合物; 其上附着有根据本发明的适体 - 结合配偶体缀合物的底物; 用于检测样品中的分析物的方法，包括使用所述适体 - 结合配偶体偶联物; 和用于缀合适体以产生适体 - 结合配偶体缀合物的结合配偶体。

公开(公告)号：WO2020069318A1

公开(公告)日：2020-04-02

申请号：PCT/US2019/053460

申请日：2019-09-27

Applicant: PURDUE RESEARCH FOUNDATION LIMITED

Inventor： YUAN, Chongli ,  WEI, Alexander

IPC: C12N15/115 ,  G01N33/00

Abstract: The present invention relates to a novel cortisol binding aptamer, the use thereof for cortisol determination as well as sensors and assays comprising the novel aptamer.

公开(公告)号：WO2017019776A1

公开(公告)日：2017-02-02

申请号：PCT/US2016/044278

申请日：2016-07-27

Applicant: PURDUE RESEARCH FOUNDATION

Inventor： WANG, Nien-Hwa, Linda ,  YUAN, Chongli ,  CRAWFORD, Morgan, H.

IPC: A61K47/42 ,  C07K1/113 ,  C07K1/14

CPC classification number: C07K1/1133

Abstract: Methods of refolding proteins are provided, especially cysteine-containing proteins such as insulin, proinsulin, and analogues thereof. The methods make used of tandem folding via the addition, at two different time, of two different reducing agents. A first reversible reducing agent can be added to induce folding and, at a later time, a second irreversible reducing agent can be added to prevent and/or reverse the formation of aggregates via intermolecular sulfide bonds. The methods can be used to reform a variety of cysteine-containing proteins with high yield of the native form, e.g. about 60 mol%, 70 mol%, or more.

Abstract translation: 提供了重折叠蛋白质的方法，特别是含有半胱氨酸的蛋白质，如胰岛素，胰岛素原及其类似物。 该方法通过在两个不同的时间添加两种不同的还原剂来使用串联折叠。 可以添加第一可逆还原剂以诱导折叠，并且在稍后的时间可以加入第二不可逆还原剂以防止和/或反转通过分子间硫键形成聚集体。 该方法可以用于以高产率的天然形式，例如改变多种含半胱氨酸的蛋白质。 约60摩尔％，70摩尔％以上。