Abstract:
The present disclosure relates to aptamers for detection of an analyte in a sample; a substrate having attached thereto the aptamers according to the invention; a kit of parts for the detection of an analyte in a sample; and a method for the detection of an analyte in a sample comprising the use of said aptamers.
Abstract:
Capture probe libraries can be used to enrich a region of interest in a sequencing library for high-depth sequencing. The capture probes within the capture probe libraries often do not function in a predictable or uniform manner. Described herein are balanced capture probe libraries and methods of balancing capture probe libraries. A sequencing library can be enriched using balanced capture probe libraries, and the enriched sequencing library can be sequenced to obtain a sequencing depth closer to a desired sequencing depth.
Abstract:
The present invention relates to a method for evaluating specificity of oligonucleotides represented by 5'-X-Y-Z-3'. The present invention comprises comparing the complete or a partial sequence of the oligonucleotide of Formula (I) against at least one database of nucleotide sequences, and extracting reference nucleotide sequences comprising a region homologous to the complete or a partial sequence of the oligonucleotide of Formula (I) from the database; and analyzing portion-by-portion match/mismatch between the oligonucleotide of Formula (I) and each of the reference nucleotide sequences to provide (i) the number or ratio of matched or mismatched bases between the portion X of the oligonucleotide of Formula (I) and each of the reference nucleotide sequences and individually (ii) the number or ratio of matched or mismatched bases between the portion Z of the oligonucleotide of Formula (I) and each of the reference nucleotide sequences.
Abstract:
The present invention relates to assays and methods for target analyte (e.g., allergen) detection in a sample using aptamer-magnetic particle complexes.
Abstract:
The present invention discloses methods for identification of oligonucleotides by manipulation of the information content a plurality of oligonucleotides. A main object of the methods is the identification of new molecular activity such as new ligands of interest for the development of therapeutics or in the field of nanotechnology.
Abstract:
Methods for screening of affinity reagents for many target proteins of interest simultaneously. Arrayed targets (e.g., peptide, protein, RNA, cell, etc.) are used in affinity selection experiments to reduce the amount of target needed and to improve the throughput of discovering recombinant affinity reagents to a large collection of targets.
Abstract:
Methods and nucleic acid molecules for detecting chromosomal abnormalities such as aneuploidy. Methods for selecting nucleic acid molecules for use in the methods of the disclosure.
Abstract:
Methods and nucleic acid molecules for detecting chromosomal abnormalities such as aneuploidy. Methods for selecting nucleic acid molecules for use in the methods of the disclosure.
Abstract:
Methods for preparing nucleic acid aptamers in the presence of a surfactant are described, as are methods for using nucleic acid aptamers to separate a target molecule from a sample. The methods may be used to separate abundant proteins including HSA from a biological sample such as serum.