EVALUATION OF SPECIFICITY OF OLIGONUCLEOTIDES
    3.
    发明申请
    EVALUATION OF SPECIFICITY OF OLIGONUCLEOTIDES 审中-公开
    评价寡核苷酸的特异性

    公开(公告)号:WO2017209575A1

    公开(公告)日:2017-12-07

    申请号:PCT/KR2017/005818

    申请日:2017-06-02

    Applicant: SEEGENE, INC.

    CPC classification number: C12Q1/68 C12Q1/6811 G16B30/00 G16B30/10 G16B50/00

    Abstract: The present invention relates to a method for evaluating specificity of oligonucleotides represented by 5'-X-Y-Z-3'. The present invention comprises comparing the complete or a partial sequence of the oligonucleotide of Formula (I) against at least one database of nucleotide sequences, and extracting reference nucleotide sequences comprising a region homologous to the complete or a partial sequence of the oligonucleotide of Formula (I) from the database; and analyzing portion-by-portion match/mismatch between the oligonucleotide of Formula (I) and each of the reference nucleotide sequences to provide (i) the number or ratio of matched or mismatched bases between the portion X of the oligonucleotide of Formula (I) and each of the reference nucleotide sequences and individually (ii) the number or ratio of matched or mismatched bases between the portion Z of the oligonucleotide of Formula (I) and each of the reference nucleotide sequences.

    Abstract translation: 本发明涉及用于评估由5'-X-Y-Z-3'表示的寡核苷酸的特异性的方法。 本发明包括将式(I)寡核苷酸的完整或部分序列与至少一个核苷酸序列数据库进行比较,并提取包含与式(I)寡核苷酸的完整或部分序列同源的区域的参考核苷酸序列 我)从数据库中; (I)的寡核苷酸与每个参考核苷酸序列之间的部分 - 部分匹配/错配以提供(i)式(I)的寡核苷酸的部分X之间的匹配或错配碱基的数目或比例 )和每个参考核苷酸序列并且单独(ii)式(I)的寡核苷酸的部分Z与每个参考核苷酸序列之间匹配的或错配的碱基的数目或比例。

Patent Agency Ranking