公开(公告)号：WO2014143696A1

公开(公告)日：2014-09-18

申请号：PCT/US2014/027764

申请日：2014-03-14

Applicant: RINKER, Kristina, D. ,  SHEPHERD, Robert, D.

Inventor： RINKER, Kristina, D. ,  SHEPHERD, Robert, D.

IPC: G01N33/483 ,  G01N33/487 ,  C12M1/00

CPC classification number: G01N33/5023 ,  B01L3/502 ,  B01L3/5025 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0627 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M35/04 ,  C12M41/46 ,  C12Q1/6876 ,  C12Q2600/158 ,  G01N33/487 ,  G01N33/5064

Abstract: Flow chambers are provided. In some embodiments, the flow chambers include an inner panel having at least one flow channel having an inlet/outlet opening on each end thereof formed therein, wherein the inlet/outlet openings are adapted to releasably receive a septum; one or more ports adapted to releasably receive a plug and for at least liquid communication with the at least one flow channel, and an outer frame that defines an outer portion of the at least one flow channel and that defines a perimeter of the flow chamber. In some embodiments, the flow chamber has overall dimensions of a standard multiwell plate and the at least one flow channel is located in a position that corresponds to a column location of the standard multiwell plate. Also provided are methods for producing the presently disclosed flow chambers and employing the same to assay biological features of cultured cells and/or tissues.

Abstract translation: 提供流动室。 在一些实施例中，流动室包括具有至少一个流动通道的内部板，所述至少一个流动通道在其每个端部上形成有入口/出口开口，其中所述入口/出口开口适于可释放地接收隔膜; 一个或多个端口，其适于可释放地接收塞子并且用于至少与所述至少一个流动通道的液体连通;以及外框架，其限定所述至少一个流动通道的外部部分并限定所述流动室的周边。 在一些实施例中，流动室具有标准多孔板的总体尺寸，并且至少一个流动通道位于对应于标准多孔板的柱位置的位置。 还提供了用于生产当前公开的流动室的方法，并且使用该方法来测定培养的细胞和/或组织的生物学特征。