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公开(公告)号：WO2017195130A1
公开(公告)日：2017-11-16
申请号：PCT/IB2017/052732
申请日：2017-05-10
IPC分类号： G01N33/68
CPC分类号： G01N33/6893 , G01N2500/00 , G01N2500/02 , G01N2800/24 , G01N2800/2821 , G01N2800/2835 , G01N2800/32 , G01N2800/52
摘要： A method for measuring autophagosome flux is provided. The autophagosome pool size in a single cell is quantified, where the pool size is the total number of autophagosomes in the cell. Fusion between the autophagosomes and lysosomes in the cell is then inhibited. The autophagosome pool size is quantified over one or more time points after fusion has been inhibited, and the autophagosome flux is calculated as the initial rate of change of the autophagosome pool size at the time point after fusion has been inhibited. The method can be used to determine basal autophagosome flux, whether a cell is diseased or dysfunctional, or to diagnose a subject with a disease, disorder or dysfunction. The transition time, the time required to clear an autophagosome pool, can also be derived from the autophagosome flux. A molecule can also be characterized according to its ability to modulate autophagosome flux in a cell.
摘要翻译： 提供了测量自噬体通量的方法。 量化单个细胞中的自噬体库大小，其中库大小是细胞中自噬体的总数。 然后抑制细胞中自噬体和溶酶体之间的融合。 在融合被抑制后，在一个或多个时间点上定量自噬体库大小，并且计算自噬体通量，作为在融合被抑制后的时间点自噬体库大小的初始变化率。 该方法可用于确定基底自噬体通量，细胞是否患病或功能障碍，或诊断患有疾病，病症或功能障碍的受试者。 过渡时间，清除自噬体池所需的时间也可以来自自噬体通量。 分子也可以根据其调节细胞自噬体通量的能力来表征。
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公开(公告)号：WO2021152562A1
公开(公告)日：2021-08-05
申请号：PCT/IB2021/050781
申请日：2021-02-01
摘要： A method of determining the location and quantity of mitochondrial fission, fusion and depolarisation events that occur in a cell is provided. Using a three-dimensional time lapse image sequence of a cell, the method identifies which of the mitochondria in a cell had depolarised or undergone fission or fusion in the interval between the acquisition of the earlier and later images, indicates the locations of the fission, fusion and depolarisation events, and generates a count of the number of mitochondrial fission, fusion and/or depolarisation events. The method can be used to diagnose a disease or condition associated with mitochondrial dysfunction, such as neurodegenerative disease, cancer or ischaemic heart disease. The method can further be used to screen a compound or composition for use in preventing or treating a disease or condition associated with mitochondrial dysfunction. The method can be computer-implemented, and a computer program product is provided.
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公开(公告)号：WO2020003286A1
公开(公告)日：2020-01-02
申请号：PCT/IB2019/055604
申请日：2019-07-01
IPC分类号： G01N33/543 , G01N27/00 , G01N33/68
摘要： A method, device and system for determining autophagic flux are claimed. The levels of proteins which change with increased or decreased autophagy are determined in a sample. The change in the level of each protein is quantified in order to obtain the autophagic flux. This can be compared to a sample flux range associated with autophagy dysfunction or ageing patterns. Diseases or conditions which may be diagnosed include neurodegenerative conditions such as Alzheimer's disease and dementia, cancer, heart conditions, immune conditions or aging-related conditions. The device for determining autophagic flux comprises a housing, receiving zones configured for receiving a substrate and a biological sample, and a set of electrodes for each receiving zone. The device is connectable to circuitry that determines an electrical property of each substrate and uses this to determine the autophagic flux.
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