公开(公告)号：WO2015149753A3

公开(公告)日：2015-11-26

申请号：PCT/DE2015100130

申请日：2015-03-27

Applicant: LEIBNIZ INST FÜR POLYMERFORSCHUNG DRESDEN E V ,  TECH UNIVERSITÄT DRESDEN

Inventor： THOMPSON MICHAEL ,  WIEDUWILD ROBERT ,  REDDAVIDE FRANCESCO ,  TSURKAN MIKHAIL ,  ANDRADE HELENA ,  WERNER CARSTEN ,  ZHANG YIXIN

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2533/50

Abstract: The invention relates to a physical self-organizing hydrogel system for biotechnological applications composed of a non-covalent network on the basis of a protein-ligand interaction, comprising a tetrameric protein as a protein and biotin or one of the derivatives thereof as a ligand, wherein biotin or one of the derivatives thereof is covalently conjugated to an end of a polymer chain of a linear or multi-arm synthetic polymer or of a single- or double-strand oligonucleotide and wherein the solid content in relation to the entire hydrogel is at least 3% and the conjugates are cross-linked by the tetrameric protein. In the hydrogel system, there is a mixture ratio as a molar equivalent ratio between the protein and the number of terminal biotinylated ends of the polymer chains in the biotin-polymer conjugate of 1:2 to 1:8. The hydrogel formation occurs with controlled kinetics on the basis of a protein-ligand interaction. The hydrogel system can be used for the cultivation and encapsulation of cells.

Abstract translation: 本发明涉及一种用于蛋白质 - 配体相互作用的基础上的非共价网络的生物技术应用物理自组织的水凝胶系统，其包括共价分别在四聚体蛋白作为蛋白和生物素或作为配体的衍生物，其中生物素或其衍生物 的直链或多臂合成聚合物或单链或双链寡核苷酸的聚合物链的一端偶联，且其中，基于总的水凝胶的固体含量为至少3％，和缀合物是由四聚体蛋白连接。 水凝胶系统由此蛋白质和在1生物素 - 聚合物缀合物的聚合物链的末端生物素化的端部的数目之间的摩尔当量比的混合比：8:2至所述第一 所述水凝胶是基于蛋白质 - 配体相互作用来控制动力学进行。 水凝胶系统可用于培养和细胞的封装。

公开(公告)号：WO2016008822A3

公开(公告)日：2016-05-19

申请号：PCT/EP2015065874

申请日：2015-07-10

Applicant: TECH UNIVERSITÄT DRESDEN

Inventor： REDDAVIDE FRANCESCO ,  DE ANDRADE HELENA ,  LIN WEILIN ,  ZHANG YIXIN ,  MELE ELISA

IPC: C40B30/04 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6811 ,  B01L3/5027 ,  B01L7/525 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/088 ,  B01L2300/1822 ,  B01L2300/1861 ,  B01L2300/1894 ,  B01L2400/0487 ,  G01N33/5308 ,  C12Q2525/205 ,  C12Q2527/101 ,  C12Q2565/629

Abstract: The present invention relates to a method for the sensitive identification of high-affinity complexes of two ligands (2, 3, 4, 5, 6, 7) and a receptor (1). To this end, a plurality of different ligands (2, 3, 4, 5, 6, 7) of a chemical library are brought into contact with at least one receptor (1) in a solution. The ligands of the library have single-stranded DNA (8, 9) or RNA with a base length of 2 to 10 bases or, alternatively, more than 10 bases. The solution is incubated for a specified period of time and complexes of two ligands (2, 3, 4, 5, 6, 7) and a receptor (1) are identified.

Abstract translation: 本发明涉及一种用于两个配体（2，3，4，5，6，7）和受体（1）的高亲和力复合物的敏感识别的处理。 这里，各种不同的配体（2，3，4，5，6，7）化学文库与至少一种受体的（1）中的溶液接触。 库的配体具有的单链DNA（8,9）或具有2至10个碱基，或可替换地大于10个碱基的碱基长度的RNA。 此外，该溶液中温育一段时间，和两个配位体的配合物（2，3，4，5，6，7）被识别和受体（1）。