公开(公告)号：WO2003012448A1

公开(公告)日：2003-02-13

申请号：PCT/US2002/024245

申请日：2002-07-30

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： GLAZER, Alexander, N. ,  TOOLEY, Aaron, J. ,  CAI, Yuping

IPC: G01N33/566

CPC classification number: C12N9/0095 ,  C07K14/43595 ,  C07K2319/21 ,  C07K2319/60 ,  C12N9/88 ,  C12N15/52 ,  C12N15/62

Abstract: Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.

Abstract translation: 描述表达荧光全血蓝蛋白融合蛋白的重组细胞及其使用方法。 所述细胞包含bilin，重组bi​​lin还原酶，包含相应的载脂蛋白结构域的融合蛋白的载脂蛋白 - 磷脂蛋白融合蛋白前体，以及组分反应形成全血蓝蛋白融合蛋白的重组体藻胆蛋白结构域 - 胆固醇裂解酶 。 还描述了基于全藻蛋白脂蛋白的转录报道细胞和测定法，其中细胞条件地表达异源细胞，荧光，第一全息型蛋白脂蛋白结构域。