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52 条记录 (耗时 0.016 秒)

    NOVEL FERREDOXIN REDUCTASE
    1.
    发明申请
    NOVEL FERREDOXIN REDUCTASE 审中-公开

    公开(公告)号：WO2019016382A1

    公开(公告)日：2019-01-24

    申请号：PCT/EP2018/069800

    申请日：2018-07-20

    申请人： DSM IP ASSETS B.V.

    发明人： CHEVREUX, Bastien ,  ROYER, John

    IPC分类号： C12N15/52 ,  A23L5/00 ,  C12N9/02

    CPC分类号： C12N9/0095 ,  A23L5/43 ,  A23L5/44 ,  C12N15/52

    摘要： The present invention is related to novel protein involved in transfer of electrons, in particular in an enzymatic conversion of carotenoids into particular retro-carotenoids, more particularly in a conversion of beta-carotene into rhodoxanthin via the catalytic action of rhodoxanthin-producing hydroxylases (hereinafter also referred to as BHYRs). The invention also features polynucleotides and the corresponding polypeptides comprising the full-length sequences of the novel gene/polypeptide and fragments thereof, in particular functional equivalents of said gene/polypeptide. The invention further relates to genetically engineered carotene producing fungal host cells and their use for biotechnological production of rhodoxanthin.

    METHODS AND COMPOSITIONS FOR EVOLVING MICROBIAL HYDROGEN PRODUCTION
    4.
    发明申请
    METHODS AND COMPOSITIONS FOR EVOLVING MICROBIAL HYDROGEN PRODUCTION 审中-公开
    用于演化微生物氢生产的方法和组合物

    公开(公告)号：WO2005072262A2

    公开(公告)日：2005-08-11

    申请号：PCT/US2005001983

    申请日：2005-01-21

    申请人： SOLAZYME INC

    发明人： DILLON HARRISON F

    IPC分类号： C12N1/12 ,  C12N1/21 ,  C12N1/36 ,  C12N9/02 ,  C12N15/01 ,  C12N15/74 ,  C12P3/00 ,  C12Q1/68

    CPC分类号： C12P3/00 ,  C12N1/12 ,  C12N1/36 ,  C12N9/0067 ,  C12N9/0095 ,  C12N15/01 ,  C12Q1/689 ,  C12Q2600/158

    摘要： The invention provides methods and compositions for engineering cells to generate large amounts of hydrogen. Genes that are involved in hydrogen production pathways and genes that are upregulated when cells are exposed to conditions conducive to the generation of hydrogen are mutagenized according to disclosed protocols. Microbes containing nucleic acid constructs are screened or selected for the ability to generate an increased amount of hydrogen. Methods of producing hydrogen are also disclosed.

    摘要翻译： 本发明提供了用于工程化细胞产生大量氢的方法和组合物。 参与氢生产途径的基因和当细胞暴露于有利于产生氢的条件时被上调的基因根据公开的方案被诱变。 筛选或选择含有核酸构建体的微生物以产生增加量的氢的能力。 还公开了生产氢的方法。

    ENGINEERED BINDING PROTEINS
    5.
    发明申请
    ENGINEERED BINDING PROTEINS 审中-公开
    工程结合蛋白

    公开(公告)号：WO03061570A3

    公开(公告)日：2003-09-18

    申请号：PCT/US0301362

    申请日：2003-01-16

    申请人： ZYOMYX INC

    发明人： WILSON DAVID S ,  NOCK STEFFEN

    IPC分类号： C07K14/195 ,  C12N9/02 ,  C12N15/07 ,  C12N15/10 ,  A61K38/00 ,  A61K45/00 ,  A61K47/00 ,  C07K1/14 ,  C07K1/16 ,  C07K14/00 ,  C12N15/03 ,  C12N15/64 ,  C12N15/65 ,  G01N33/53 ,  G01N33/566

    CPC分类号： C12N15/1093 ,  B82Y30/00 ,  C07K14/195 ,  C12N9/0095

    摘要： Engineered binding proteins are provided. In some cases, the parent protein corresponding to the engineered protein has a three-layer swiveling beta/beta/alpha domain. In other cases, the parent protein corresponding to the engineered protein has a rubredoxin like fold. At least one portion of the primary sequence of the engineered protein is determined by an engineering scheme. In some case, the engineered protein is characterized by an ability to bind to a compound that the parent protein does not bind. In some cases, the parent protein is derived from a domain of a chaperonin or a rubredoxin. One form of engineering scheme used is a randomization scheme. A method for making libraries of engineered proteins, all based on a single parent protein is provided. Methods to identify proteins that bind to compounds of interest in libraries of engineered libraries is provided. An array of engineered proteins immobilized on a support is provided. Each engineered protein in the array is a chaperonin domain or a rubredoxin that has been subjected to an engineering scheme.

    摘要翻译： 提供工程结合蛋白。 在某些情况下，对应于工程化蛋白质的亲本蛋白质具有三层旋转β/β/α结构域。 在其他情况下，对应于工程改造的蛋白质的亲本蛋白质具有类似于rubredoxin的折叠。 工程化蛋白质的初级序列的至少一部分由工程方案确定。 在某些情况下，工程改造的蛋白质的特征在于与母体蛋白不结合的化合物结合的能力。 在一些情况下，母体蛋白质衍生自伴侣蛋白或红蛋白的结构域。 使用的一种工程方案是一种随机化方案。 提供了基于单亲蛋白的制备工程蛋白的文库的方法。 提供了在工程化文库库中鉴定与目的化合物结合的蛋白质的方法。 提供固定在载体上的工程蛋白阵列。 阵列中的每种工程化蛋白质都是经过工程方案的伴侣蛋白结构域或红霉素。

    ENGINEERING OF LIVING CELLS FOR THE EXPRESSION OF HOLO-PHYCOBILIPROTEIN-BASED CONSTRUCTS
    6.
    发明申请
    ENGINEERING OF LIVING CELLS FOR THE EXPRESSION OF HOLO-PHYCOBILIPROTEIN-BASED CONSTRUCTS 审中-公开
    用于表达HOLO-PHYCOBILIPROTEIN的结构的生活细胞工程

    公开(公告)号：WO2003012448A1

    公开(公告)日：2003-02-13

    申请号：PCT/US2002/024245

    申请日：2002-07-30

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： GLAZER, Alexander, N. ,  TOOLEY, Aaron, J. ,  CAI, Yuping

    IPC分类号： G01N33/566

    CPC分类号： C12N9/0095 ,  C07K14/43595 ,  C07K2319/21 ,  C07K2319/60 ,  C12N9/88 ,  C12N15/52 ,  C12N15/62

    摘要： Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.

    摘要翻译： 描述表达荧光全血蓝蛋白融合蛋白的重组细胞及其使用方法。 所述细胞包含bilin，重组bi​​lin还原酶，包含相应的载脂蛋白结构域的融合蛋白的载脂蛋白 - 磷脂蛋白融合蛋白前体，以及组分反应形成全血蓝蛋白融合蛋白的重组体藻胆蛋白结构域 - 胆固醇裂解酶 。 还描述了基于全藻蛋白脂蛋白的转录报道细胞和测定法，其中细胞条件地表达异源细胞，荧光，第一全息型蛋白脂蛋白结构域。