公开(公告)号：WO2021154985A1

公开(公告)日：2021-08-05

申请号：PCT/US2021/015475

申请日：2021-01-28

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： FAN, Xudong ,  WANG, Xueding ,  LI, Xuzhou ,  ZHANG, Wei ,  WANG, William Yanli ,  WU, Xiaoqin ,  TAN, Xiaotian ,  BAKER, Brendon ,  PAULUS, Yannis M.

IPC: G01N21/64 ,  G01N21/62 ,  G01N21/63

Abstract: An imaging system for cell-based therapies is provided. The imagining system includes one or more optical tags configured for insertion into a cell or biological tissue, an excitation light source configured to illuminate the one or more optical tags; a detector configured to measure optical emission of the one or more optical tags; an imaging subsystem configured to determine a three-dimensional location of each of the one or more optical tags in the cell or biological tissue; and a controller in electrical communication with the excitation light source, the detector, and the imaging subsystem. Each of the one or more optical tags has a contrasting feature and includes a fluorescent material. The contrasting feature may be defined by at least one of a refractive index, shape, color, and laser emission of each optical tag of the one or more optical tags.

公开(公告)号：WO2021142439A1

公开(公告)日：2021-07-15

申请号：PCT/US2021/012949

申请日：2021-01-11

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN ,  ARBORSENSE INC. ,  OPTOFLUIDIC BIOASSAY, LLC

Inventor： FAN, Xudong ,  XUE, Wen ,  TAN, Xiaotian ,  ILGEN, Mark, A. ,  KULKARNI, Girish ,  KHAING OO, Maung, Kyaw

IPC: G01N21/64 ,  G01N33/533 ,  G01N33/543

Abstract: An automated microfluidic bioreactor device and methods to rapidly detect drugs of abuse from human sweat are provided. The bioreactor can perform either single-plexed measurements (detecting only one target analyte at a time) or multiplexed measurement (detecting multiple analytes simultaneously). The bioreactor device has a cartridge comprising a capillary array that employs competitive enzyme-linked immunosorbent assay (ELISA) to detect the presence of various drugs or metabolite compounds. For example, four common drugs, methadone, methamphetamine, amphetamine, and tetrahydrocannabinol, were detected rapidly and quantitatively in about 16 minutes with a low sweat sample volume (about 4 μL per analyte) and a large dynamic range (methadone: 0.0016 ng/mL- 1 ng/mL; METH: 0.016 ng/mL - 25 ng/mL; amphetamine: 0.005 ng/mL - 10 ng/mL; THC: 0.02 ng/mL - 1000 ng/mL).

公开(公告)号：WO2020146637A1

公开(公告)日：2020-07-16

申请号：PCT/US2020/012939

申请日：2020-01-09

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： FAN, Xudong ,  WANG, Xueding ,  LI, Xuzhou ,  QIN, Yu ,  TAN, Xiaotian ,  CHEN, Yu-Cheng ,  CHEN, Qiushu

IPC: H01S3/213 ,  H01S3/20 ,  H01S3/10 ,  H01S3/08 ,  H01S3/094 ,  G01N21/62 ,  G01N21/63 ,  C09K11/06

Abstract: A laser whose emission is modulated by ultrasound is presented. The laser is usually micron-sized. In response to ultrasound modulation, the laser emission increases and decreases. Such a change in emission can be detected by external optical detectors. This type of laser can be used as a new type of imaging modality, in which laser emission in combination with sound waves or ultrasound waves, is used for imaging. Laser emission has a much narrower spectral linewidth and stronger intensity than fluorescence and therefore is able to achieve higher sensitivity, whereas sound waves are used to provide a better spatial resolution of the laser emission from the laser. In ultrasound modulated laser based imaging, multiple lasers can be placed inside cells or tissues.