公开(公告)号：WO2020146637A1

公开(公告)日：2020-07-16

申请号：PCT/US2020/012939

申请日：2020-01-09

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： FAN, Xudong ,  WANG, Xueding ,  LI, Xuzhou ,  QIN, Yu ,  TAN, Xiaotian ,  CHEN, Yu-Cheng ,  CHEN, Qiushu

IPC: H01S3/213 ,  H01S3/20 ,  H01S3/10 ,  H01S3/08 ,  H01S3/094 ,  G01N21/62 ,  G01N21/63 ,  C09K11/06

Abstract: A laser whose emission is modulated by ultrasound is presented. The laser is usually micron-sized. In response to ultrasound modulation, the laser emission increases and decreases. Such a change in emission can be detected by external optical detectors. This type of laser can be used as a new type of imaging modality, in which laser emission in combination with sound waves or ultrasound waves, is used for imaging. Laser emission has a much narrower spectral linewidth and stronger intensity than fluorescence and therefore is able to achieve higher sensitivity, whereas sound waves are used to provide a better spatial resolution of the laser emission from the laser. In ultrasound modulated laser based imaging, multiple lasers can be placed inside cells or tissues.

公开(公告)号：WO2021222718A1

公开(公告)日：2021-11-04

申请号：PCT/US2021/030112

申请日：2021-04-30

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： FAN, Xudong ,  CHEN, Qiushu ,  SUN, Yunlu

IPC: G01N21/64

Abstract: The present disclosure provides an imaging system. The imaging system may include a laser cavity that is configured to receive a biological sample, where the biological sample is treated with a dye; an excitation light source that is configured to direct energy at the laser cavity so as to cause an emission from the biological sample, where the emission includes a laser emission at a first spectral band and a fluorescence emission at a second spectral band; a first detector that is configured to measure the laser emission generated by the biological sample; a second detector that is configured to measure the fluorescence emission generated by the biological sample; a splitter that is configured to direct the laser emission to the first detector and the fluorescence emission to the second detector; and a controller interfaced with the excitation light source, the first detector, and the second detector.

公开(公告)号：WO2018125925A1

公开(公告)日：2018-07-05

申请号：PCT/US2017/068556

申请日：2017-12-27

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： FAN, Xudong ,  CHEN, Yu-Cheng ,  CHEN, Qiushu

IPC: G01N21/64 ,  G02B21/00

Abstract: Laser emission based microscope devices and methods of using such devices for detecting laser emissions from a tissue sample are provided. The scanning microscope has first and second reflection surfaces and a scanning cavity holding a stationary tissue sample with at least one fluorophore/lasing energy responsive species. At least a portion of the scanning cavity corresponds to a high quality factor (Q) Fabry-Pérot resonator cavity. A lasing pump source directs energy at the scanning cavity while a detector receives and detects emissions generated by the fluorophore(s) or lasing energy responsive species. The second reflection surface and/or the lasing pump source are translatable with respect to the stationary tissue sample for generating a two-dimensional scan of the tissue sample. Methods for detecting multiplexed emissions or quantifying one or more biomarkers in a histological tissue sample, for example for detection and diagnosis of cancer, or other disorders/diseases are provided.