公开(公告)号：WO2020251697A2

公开(公告)日：2020-12-17

申请号：PCT/US2020/030795

申请日：2020-04-30

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： CORNISH, Virginia ,  BRISBOIS, James ,  BILLERBECK, Sonja ,  JIMENEZ, Miguel

IPC: C07K14/72 ,  C12N1/14 ,  C12N15/81 ,  C12N15/90 ,  C07K14/37 ,  C07K14/38 ,  C07K14/385 ,  C07K14/39 ,  C07K14/395 ,  C07K14/40 ,  C12N1/20 ,  C12P21/02 ,  H01L49/00 ,  H01L51/00

Abstract: The present disclosure relates to intercellular signaling between genetically-engineered cells and, more specifically, to a scalable peptide-GPCR intercellular signaling system. The present disclosure provides an intercellular signaling system that includes at least two cells that have been genetically-engineered to communicate with each other, methods of use and kits thereof.

公开(公告)号：WO2020124093A1

公开(公告)日：2020-06-18

申请号：PCT/US2019/066634

申请日：2019-12-16

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： ANZALONE, Andrew, V. ,  CORNISH, Virginia, W. ,  JIMENEZ, Miguel

IPC: C12N5/10 ,  C12N15/09 ,  C12N15/62 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/58

Abstract: The disclosed subject matter relates to nucleic acid constructs that encode one or more fluorescent proteins, which can function as intracellular fluorescent tags, specifically for flow cytometry and fluorescence microscopy. The present disclosure further provides genetically-engineered cells that include one or more nucleic acid constructs and methods for using such genetically-engineered cells and nucleic acid constructs.

公开(公告)号：WO2016081619A1

公开(公告)日：2016-05-26

申请号：PCT/US2015/061373

申请日：2015-11-18

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： CORNISH, Virginia, Wood ,  OSTROV, Nili ,  JIMENEZ, Miguel ,  BILLERBECK, Sonja

IPC: C12Q1/02 ,  C12N15/09 ,  G01N33/566

CPC classification number: G01N33/566 ,  C12Q1/005 ,  C12Q1/02 ,  C12Q1/025 ,  C12Q1/06 ,  G01N33/5023 ,  G01N33/5038 ,  G01N33/569 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/5695 ,  G01N33/56961 ,  G01N33/74 ,  G01N2333/726

Abstract: The present invention provides sensor cells comprising a receptor that binds to an analyte indicative of the presence of an agent, where binding of the analyte to the receptor triggers a detection event that is indicative of the presence of the agent. In certain embodiments, the detection event is appearance of a reporter detectable by the naked eye. The present invention also provides uses of such sensor cells for detecting the presence of an agent in a sample.

Abstract translation: 本发明提供了包含受体的传感器细胞，所述受体结合指示试剂存在的分析物，其中分析物与受体的结合触发指示试剂存在的检测事件。 在某些实施方案中，检测事件是肉眼可检测的报告人的外观。 本发明还提供了这种传感器电池用于检测样品中药剂的存在的用途。