公开(公告)号：WO2016043903A1

公开(公告)日：2016-03-24

申请号：PCT/US2015/045712

申请日：2015-08-18

申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

发明人： SOPER, Steven, A. ,  UBA, Franklin, I. ,  WEERAKOON-RATNAYAKE, Kumuditha, M. ,  CALIXTE, Nyote, J.

IPC分类号： C12M1/14 ,  B29C65/02 ,  B32B33/00 ,  B32B3/10 ,  B82Y40/00

CPC分类号： B29C65/02 ,  B01L3/502707 ,  B01L2300/04 ,  B01L2300/0887 ,  B29C66/028 ,  B29C66/1122 ,  B29C66/53461 ,  B29C66/71 ,  B29C66/712 ,  B29C66/73118 ,  B29C66/73921 ,  B29L2031/7562 ,  B32B3/30 ,  B32B7/02 ,  B32B27/08 ,  B32B27/16 ,  B32B27/308 ,  B32B27/325 ,  B32B37/04 ,  B32B2307/30 ,  B32B2307/50 ,  B81C99/009 ,  B82Y30/00 ,  B82Y40/00 ,  B29K2023/38 ,  B29K2033/12

摘要： A method of making a nanofluidic device is carried out by providing a thermoplastic substrate having a top surface portion, the top surface portion having at least one nanofluidic feature formed therein; providing a thermoplastic cover having a bottom surface portion, the thermoplastic cover having a glass transition temperature less than that of the substrate; optionally activating one or both of the thermoplastic substrate top surface portion and the thermoplastic cover bottom surface portion; and then thermally bonding the thermoplastic cover top surface portion to the thermoplastic cover bottom surface portion to produce the nanofluidic device.

摘要翻译： 制造纳米流体装置的方法是通过提供具有顶表面部分的热塑性基材来进行的，顶表面部分具有形成在其中的至少一个纳米流体特征; 提供具有底表面部分的热塑性盖，所述热塑性盖的玻璃化转变温度小于所述基材的玻璃化转变温度; 可选地激活热塑性基材顶表面部分和热塑性盖底面部分中的一个或两个; 然后将热塑性盖顶表面部分热粘合到热塑性盖底表面部分上，以制造纳米流体装置。

公开(公告)号：WO2016154302A1

公开(公告)日：2016-09-29

申请号：PCT/US2016/023769

申请日：2016-03-23

申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  CORNELL UNIVERSITY ,  BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE

发明人： SOPER, Steven, A. ,  BARANY, Francis ,  PARK, Sunggook ,  MURPHY, Michael ,  MCKINNEY, Collin, J. ,  EFCAVITCH, John, William ,  HUPERT, Mateusz

IPC分类号： B01L1/00 ,  B01L99/00 ,  C40B30/04 ,  C12M1/40 ,  G01N33/483 ,  G01N33/569

CPC分类号： G01N33/48721 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0896 ,  C12M1/40 ,  C12M23/16 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2565/50 ,  C12Q2565/631

摘要： The present invention is directed to a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The device also comprises one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber.

摘要翻译： 本发明涉及包含生物分子处理器和一个或多个纳米管的装置。 每个生物分子处理器包括由固体基底限定的生物反应器室，在所述生物反应器室内的多个间隔开的支撑结构并连接到固体基底，以及一个或多个固定在所述多个间隔开的支撑结构中的一些或全部的捕获分子，所述捕获分子 一种或多种适于结合样品中靶核酸分子的一部分的捕获分子。 该装置还包括由固体基质定义并流体耦合到生物反应器室的一个或多个纳米管。

公开(公告)号：WO2016154337A2

公开(公告)日：2016-09-29

申请号：PCT/US2016/023814

申请日：2016-03-23

申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL ,  CORNELL UNIVERSITY ,  BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE

发明人： BARANY, Francis ,  EFCAVITCH, John, William ,  SOPER, Steven, A. ,  PARK, Sunggook

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q2561/125 ,  C12Q2565/501 ,  C12Q2565/631 ,  C12Q2531/125 ,  C12Q2535/125 ,  C12Q2521/301 ,  C12Q2521/331 ,  C12Q2561/109

摘要： The present invention is directed methods for identifying, in a sample, one or more target nucleotide sequences differing from other nucleotide sequences in the sample by one or more nucleotides, one or more copy numbers, one or more transcript sequences, and/or one or more methylated residues, using ligation detection reactions, polymerase mediated extension reactions, and/or cleavage reactions. The present invention is also directed to methods for identifying, in a sample, one or more nucleotides in a target nucleotide sequence.

摘要翻译： 本发明是用于在样品中鉴定一个或多个与一个或多个核苷酸，一个或多个拷贝数，一个或多个转录本序列和/或一个或多个核苷酸的拷贝数， 更多的甲基化残基，使用连接检测反应，聚合酶介导的延伸反应和/或裂解反应。 本发明还涉及在样品中鉴定靶核苷酸序列中的一个或多个核苷酸的方法。